A new method for identification of proteins separated in Polyacrylamide gels

1982 ◽  
Vol 10 (1) ◽  
pp. 32-33 ◽  
Author(s):  
M. H. JOHNSON ◽  
R. W. H. WALKER ◽  
G. KEIR ◽  
E. J. THOMPSON
Keyword(s):  
New Method ◽  
Polyacrylamide Gels

A new method for the detection of enolase activity on polyacrylamide gels

1979 ◽  
Vol 98 (1) ◽  
pp. 226-230 ◽  
Author(s):  
H.K. Sharma ◽  
Morton Rothstein
Keyword(s):  
New Method ◽  
Polyacrylamide Gels

Concanavalin A-horseradish peroxidase bridge staining of alpha-1 glycoproteins separated by isoelectric focusing on polyacrylamide gel.

1976 ◽  
Vol 24 (8) ◽  
pp. 908-914 ◽  
Author(s):  
R C Allen ◽  
S S Spicer ◽  
D Zehr
Keyword(s):  
Horseradish Peroxidase ◽  
Isoelectric Focusing ◽  
Concanavalin A ◽  
Periodic Acid ◽  
Polyacrylamide Gel ◽  
New Method ◽  
Polyacrylamide Gels ◽  
Periodic Acid Schiff ◽  
Coomassie Blue

The Coomassie Blue protein stain and the periodic acid-Schiff stain for glycoproteins are compared to a new method of staining glycoproteins resolved electrophoretically. The method utilizes a Concanavalin A-horseradish peroxidase sequence to visualize selectively glycoproteins with terminal or internal mannose or terminal N-acetylglucosamine. The method applied to characterization of M and Z allele products of alpha-l-antitrypsins separated by isoelectric focusing of polyacrylamide gels slabs have revealed differences in carbohydrate content of various components that were previously undetected.

Carbonic anhydrase: A new method of detection on polyacrylamide gels using low-temperature fluorescence

1971 ◽  
Vol 44 (2) ◽  
pp. 388-391 ◽  
Author(s):  
B.D. Patterson ◽  
C.A. Atkins ◽  
D. Graham ◽  
R.B.H. Wills
Keyword(s):  
Low Temperature ◽  
Carbonic Anhydrase ◽  
New Method ◽  
Polyacrylamide Gels

scholarly journals Preparation, proteolysis and reversible oxidation of highly purified Azotobacter vinelandii polynucleotide phosphorylase

1970 ◽  
Vol 120 (4) ◽  
pp. 753-761 ◽  
Author(s):  
A. T. Gajda ◽  
G. Zaror de Behrens ◽  
P. S. Fitt
Keyword(s):  
Electrophoretic Mobility ◽  
Good Yield ◽  
Azotobacter Vinelandii ◽  
New Method ◽  
Reduced Form ◽  
Polyacrylamide Gels ◽  
Polynucleotide Phosphorylase ◽  
Oligonucleotide Primers ◽  
Aerial Oxidation

1. A new method has been developed for the preparation in good yield of highly purified Azotobacter vinelandii polynucleotide phosphorylase in its reduced form. 2. Aging or digestion with trypsin causes the enzyme to develop a primer requirement that is not eliminated by β-mercaptoethanol. 3. The development of a primer requirement is accompanied by marked changes of the electrophoretic mobility of the enzyme in polyacrylamide gels. 4. The enzyme is inactivated by aerial oxidation or thiol-specific reagents. The lost activity is restored by β-mercaptoethanol, but not by oligonucleotide primers.

Selective Sampling and Orientation of Non-Homogeneous Tissues

1968 ◽  
Vol 26 ◽  
pp. 98-99
Author(s):  
C. C. Clawson ◽  
L. W. Anderson ◽  
R. A. Good
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Light Microscopy ◽  
Random Sampling ◽  
New Method ◽  
Microscope Examination ◽  
Small Areas ◽  
Selective Sampling ◽  
Large Numbers ◽  
Specific Orientation

Investigations which require electron microscope examination of a few specific areas of non-homogeneous tissues make random sampling of small blocks an inefficient and unrewarding procedure. Therefore, several investigators have devised methods which allow obtaining sample blocks for electron microscopy from region of tissue previously identified by light microscopy of present here techniques which make possible: 1) sampling tissue for electron microscopy from selected areas previously identified by light microscopy of relatively large pieces of tissue; 2) dehydration and embedding large numbers of individually identified blocks while keeping each one separate; 3) a new method of maintaining specific orientation of blocks during embedding; 4) special light microscopic staining or fluorescent procedures and electron microscopy on immediately adjacent small areas of tissue.

A new method for the determination of nitrates

1960 ◽  
Vol 23 ◽  
pp. 227-232 ◽  
Author(s):  
P WEST ◽  
G LYLES
Keyword(s):  
New Method
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