Bovine thymus satellite I DNA sequences, which are highly methylated, are preferentially located in H1-rich chromatin

1988 ◽  
Vol 66 (4) ◽  
pp. 256-261 ◽  
Author(s):  
James R. Davie ◽  
Genevieve P. Delcuve
Keyword(s):  
Dna Methylation ◽  
Dna Sequence ◽  
Dna Sequences ◽  
Single Copy ◽  
Nucleosomal Dna ◽  
Copy Dna ◽  
Single Copy Dna

The distribution of 5-methylcytosine among H1-rich and -poor bovine thymus chromatin regions was determined. 5-Methylcytosine was enriched in H1-rich chromatin regions, with linker and nucleosomal DNA containing similar amounts of this modified base. Satellite I DNA sequences, which constitute 5–7% of the genome and are highly methylated, were preferentially localized among H1-rich chromatin regions, in accordance with the distribution of 5-methylcytosine. In contrast to the satellite I DNA sequences, prothrombin (a single copy DNA sequence) was localized among both H1-rich and -poor chromatin regions. The results of this study are consistent with the hypothesis that DNA methylation has a role in modulating the structure of chromatin.

Electron microscopy of Achlya deoxyribonucleic acid sequence organization

1981 ◽  
Vol 1 (2) ◽  
pp. 136-143
Author(s):  
M Pellegrini ◽  
W E Timberlake ◽  
R B Goldberg
Keyword(s):  
Dna Sequences ◽  
Deoxyribonucleic Acid ◽  
Microscopic Analysis ◽  
Single Copy ◽  
Electron Microscopic ◽  
Sequence Organization ◽  
Double Stranded Dna ◽  
Copy Dna ◽  
Gene 32 ◽  
Single Copy Dna

Electron microscopic analysis of reassociated deoxyribonucleic acid (DNA) from the aquatic fungus Achlya bisexualis revealed details of the sequence arrangement of the inverted repeats and both the highly and moderately repetitive sequence clusters. We used the gene 32 protein-ethidium bromide technique for visualizing the DNA molecules, a procedure which provides excellent contrast between single- and double-stranded DNA regions. Long (greater than 6-kilobase) DNA fragments were isolated after reannealing to two different repetitive C0t values, and the renatured structures were then visualized in an electron microscope. Our results showed that the inverted repeat sequences were short (0.5 kilobase, number-average) and separated by nonhomologous DNA of various lengths. These pairs of sequences were not clustered within the genome. Both highly repetitive and moderately repetitive DNA sequences were organized as tandem arrays of precisely paired, regularly repeating units. No permuted clusters of repeating sequences were observed, nor was there evidence of interspersion of repetitive with single-copy DNA sequences in the Achlya genome.

scholarly journals Genomic distribution of repeated and single copy DNA sequences inPenicillium funiculosum

1987 ◽  
Vol 40 (2-3) ◽  
pp. 315-319 ◽  
Author(s):  
N.A. Sahasrabudhe ◽  
M.N. Sainani ◽  
V.S. Gupta ◽  
P.K. Ranjekar
Keyword(s):  
Dna Sequences ◽  
Single Copy ◽  
Genomic Distribution ◽  
Copy Dna ◽  
Single Copy Dna

scholarly journals Molecular and chromosomal characterization of repeated and single-copy DNA sequences in the genome of Dasypyrum villosum

Hereditas ◽  
2008 ◽  
Vol 116 ◽  
pp. 55-65 ◽  
Author(s):  
C. DE PACE ◽  
V. DELRE ◽  
G. T. SCARASCIA MUGNOZZA ◽  
C. O. QUALSET ◽  
R. CREMONINI ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Single Copy ◽  
Dasypyrum Villosum ◽  
Copy Dna ◽  
Single Copy Dna

Physical localization of single-copy sequences on pachytene chromosomes in maize (Zea mays L.) by chromosome in situ suppression hybridization

Genome ◽  
2000 ◽  
Vol 43 (6) ◽  
pp. 1081-1083 ◽  
Author(s):  
Monther T Sadder ◽  
Norbert Ponelies ◽  
Ute Born ◽  
Gerd Weber
Keyword(s):  
Zea Mays ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Dna Sequence ◽  
Zea Mays L ◽  
Single Copy ◽  
Chromosome 9 ◽  
Copy Dna ◽  
Single Copy Dna

A new approach for locating single-copy DNA sequences on pachytene chromosomes of maize (Zea mays L.) was developed. A cosmid clone with homologous sequences to a molecular marker (umc105a) linked to a quantitative trait locus (QTL) for resistance against sugarcane borer (SCB) was physically mapped by fluorescence in situ hybridization (FISH) to the short arm of chromosome 9. The marker umc105a was genetically placed in the centromeric region. To suppress signals generated by maize repetitive DNA, competitive in situ suppression (CISS) hybridization was necessary to obtain specific signals from umc105a. A centromere specific DNA probe (CentC) was used in a double-labeling technique as a reference marker. Fluorescence signals generated by umc105a cosmid and CentC were specific and highly reproducible. Thus the single-copy DNA sequence of umc105a was physically localized on the short arm of chromosome 9 near the telomere. This is the first report of physical localization of single-copy DNA sequence by CISS hybridization to a maize pachytene chromosome.Key words: fluorescence in situ hybridization, maize, pachytene chromosome, single-copy sequence, CISS hybridization.

scholarly journals Electron microscopy of Achlya deoxyribonucleic acid sequence organization.

1981 ◽  
Vol 1 (2) ◽  
pp. 136-143 ◽  
Author(s):  
M Pellegrini ◽  
W E Timberlake ◽  
R B Goldberg
Keyword(s):  
Dna Sequences ◽  
Deoxyribonucleic Acid ◽  
Microscopic Analysis ◽  
Single Copy ◽  
Electron Microscopic ◽  
Sequence Organization ◽  
Double Stranded Dna ◽  
Copy Dna ◽  
Gene 32 ◽  
Single Copy Dna

Electron microscopic analysis of reassociated deoxyribonucleic acid (DNA) from the aquatic fungus Achlya bisexualis revealed details of the sequence arrangement of the inverted repeats and both the highly and moderately repetitive sequence clusters. We used the gene 32 protein-ethidium bromide technique for visualizing the DNA molecules, a procedure which provides excellent contrast between single- and double-stranded DNA regions. Long (greater than 6-kilobase) DNA fragments were isolated after reannealing to two different repetitive C0t values, and the renatured structures were then visualized in an electron microscope. Our results showed that the inverted repeat sequences were short (0.5 kilobase, number-average) and separated by nonhomologous DNA of various lengths. These pairs of sequences were not clustered within the genome. Both highly repetitive and moderately repetitive DNA sequences were organized as tandem arrays of precisely paired, regularly repeating units. No permuted clusters of repeating sequences were observed, nor was there evidence of interspersion of repetitive with single-copy DNA sequences in the Achlya genome.

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