The Role of Adenosine Triphosphate in the Regulation of Free Calcium Ion Concentrations by Mitochondria from Guinea-Pig Cerebral Cortex

1979 ◽  
Vol 7 (5) ◽  
pp. 1126-1126
Author(s):  
DAVID G. NICHOLLS ◽  
IAN D. SCOTT
Keyword(s):  
Cerebral Cortex ◽  
Guinea Pig ◽  
Adenosine Triphosphate ◽  
Calcium Ion ◽  
Free Calcium ◽  
Ion Concentrations

scholarly journals Oscillatory membrane potential changes in cells of mesenchymal origin: the role of an intracellular calcium regulating system

1979 ◽  
Vol 81 (1) ◽  
pp. 49-61
Author(s):  
P. G. Nelson ◽  
M. P. Henkart
Keyword(s):  
Endoplasmic Reticulum ◽  
Membrane Potential ◽  
Intracellular Calcium ◽  
Calcium Ion ◽  
Surface Membrane ◽  
Ion Concentration ◽  
Free Calcium ◽  
Potassium Ions ◽  
Calcium Ion Concentration

A number of mesenchymal cells (fibroblasts, macrophages and megakaryocytes) respond to a variety of stimuli with large hyperpolarizations lasting several seconds (the H.A. response). The H.A. responses can occur as repetitive trains or oscillations. These hyperpolarizations are due to an increase of the surface membrane permeability to potassium ions which is probably mediated by an increase in the cytoplasmic free calcium ion concentration. Evidence is discussed which suggests that the source of this increased calcium, is least in part, an intracellular sequestering system, probably the endoplasmic reticulum. A model capable of producing oscillatory changes in membrane potential is proposed based on such an intracellular calcium sequestering and releasing system.

scholarly journals Spontaneous Oscillations of Cytoplasmic Free Calcium Ion Concentration in Cultured Smooth Muscle Cells from Guinea Pig Ileum

1993 ◽  
Vol 63 (1) ◽  
pp. 83-91 ◽  
Author(s):  
Hisayuki Ohata ◽  
Toru Kawanishi ◽  
Megumi Kawanishi ◽  
Chikako Uneyama ◽  
Michihito Takahashi ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Smooth Muscle Cells ◽  
Calcium Ion ◽  
Muscle Cells ◽  
Ion Concentration ◽  
Free Calcium ◽  
Guinea Pig Ileum ◽  
Cultured Smooth Muscle Cells ◽  
Calcium Ion Concentration

scholarly journals Measurement of intrasynaptosomal free calcium by using the fluorescent indicator quin-2

1984 ◽  
Vol 219 (1) ◽  
pp. 149-158 ◽  
Author(s):  
R H Ashley ◽  
M J Brammer ◽  
R Marchbanks
Keyword(s):  
Cerebral Cortex ◽  
Plasma Membrane ◽  
Guinea Pig ◽  
Calcium Ionophore ◽  
Ionized Calcium ◽  
Free Calcium ◽  
Acetoxymethyl Ester ◽  
Fluorescent Indicator ◽  
Calcium Indicator ◽  
Hydrolysis Of

The recently synthesized calcium indicator quin −2 was incorporated into synaptosomes from guinea-pig cerebral cortex following uptake and internal hydrolysis of quin −2 tetra-acetoxymethyl ester. Incubation in physiological media containing 1 mM- or 2 mM-CaCl2 led to equilibrium cytosolic ionized calcium concentrations of 85 +/- 10 nM and 205 +/- 5 nM respectively (mean +/- S.E.M. from eight and eighteen preparations respectively). Cytosolic Ca2+ was elevated following increases in external Ca2+ concentration, plasma membrane depolarization, mitochondrial inhibition, calcium ionophore addition or replacement of external sodium by lithium. Preliminary experiments were performed to assess changes in cytosolic Ca2+ accompanying the release of the neurotransmitter acetylcholine.

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