Carbachol- and KCl-induced changes in phosphoinositide metabolism and free calcium in guinea pig cerebral cortex synaptosomes

1993 ◽  
Vol 18 (5) ◽  
pp. 579-583
Author(s):  
Maija-Riitta Hirvonen ◽  
Hannu Komulainen ◽  
Kai Savolainen
Keyword(s):  
Cerebral Cortex ◽  
Guinea Pig ◽  
Free Calcium ◽  
Phosphoinositide Metabolism ◽  
Induced Changes

scholarly journals Measurement of intrasynaptosomal free calcium by using the fluorescent indicator quin-2

1984 ◽  
Vol 219 (1) ◽  
pp. 149-158 ◽  
Author(s):  
R H Ashley ◽  
M J Brammer ◽  
R Marchbanks
Keyword(s):  
Cerebral Cortex ◽  
Plasma Membrane ◽  
Guinea Pig ◽  
Calcium Ionophore ◽  
Ionized Calcium ◽  
Free Calcium ◽  
Acetoxymethyl Ester ◽  
Fluorescent Indicator ◽  
Calcium Indicator ◽  
Hydrolysis Of

The recently synthesized calcium indicator quin −2 was incorporated into synaptosomes from guinea-pig cerebral cortex following uptake and internal hydrolysis of quin −2 tetra-acetoxymethyl ester. Incubation in physiological media containing 1 mM- or 2 mM-CaCl2 led to equilibrium cytosolic ionized calcium concentrations of 85 +/- 10 nM and 205 +/- 5 nM respectively (mean +/- S.E.M. from eight and eighteen preparations respectively). Cytosolic Ca2+ was elevated following increases in external Ca2+ concentration, plasma membrane depolarization, mitochondrial inhibition, calcium ionophore addition or replacement of external sodium by lithium. Preliminary experiments were performed to assess changes in cytosolic Ca2+ accompanying the release of the neurotransmitter acetylcholine.

scholarly journals Turnover of protein-bound serine phosphate in respiring slices of guinea-pig cerebral cortex. Effects of putative transmitters, tetrodotoxin and other agents

1973 ◽  
Vol 132 (3) ◽  
pp. 475-482 ◽  
Author(s):  
Martin Reddington ◽  
Richard Rodnight ◽  
Michael Williams
Keyword(s):  
Cerebral Cortex ◽  
Cyclic Amp ◽  
Guinea Pig ◽  
Electrical Pulses ◽  
Phosphorus Turnover ◽  
Serine Phosphate ◽  
Stimulation Of

1. The effect of various agents on the turnover of protein-bound phosphorus in respiring slices of cerebral cortex was studied. 2. Confirming previous work turnover was increased by the application of electrical pulses for 10s to the tissue. 3. Turnover was also increased by exposure of the slices for 10min to noradrenaline (0.5mm), 5-hydroxytryptamine (1μm) and histamine (0.1mm). 4. When slices were stimulated by electrical pulses in the presence of histamine the increase in turnover was the sum of the responses given by each agent above, suggesting that different phosphorylating systems were involved. 5. Tetrodotoxin (0.5μm) blocked the increased turnover due to electrical pulses, but not that due to histamine. Tetrodotoxin also prevented the increase in tissue cyclic AMP content caused by the application of electrical pulses. 6. Phosphoprotein turnover was not affected by adenosine, despite the increase in tissue cyclic AMP content given by this agent. 7. Adenosine blocked the phosphoprotein response to histamine, but did not affect the response to electrical pulses. 8. The results are discussed in relation to the hypothesis that the stimulation of protein phosphorus turnover by electrical pulses is secondary to the release of cyclic AMP in the tissue.

Olprinone for the Treatment, but Not Prevention, of Fatigue-Induced Changes in Guinea-Pig Diaphragmatic Contractility

2003 ◽  
pp. 1679-1782 ◽  
Author(s):  
Aki Uemura ◽  
Yoshitaka Fujii ◽  
Hidenori Toyooka ◽  
Setsuko Suzuki ◽  
Kohei Sawada ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Induced Changes

Drug induced changes in free, labile and stable acetylcholine of guinea-pig brain

1969 ◽  
Vol 18 (6) ◽  
pp. 1315-1324 ◽  
Author(s):  
L. Beani ◽  
C. Bianchi ◽  
P. Megazzini ◽  
L. Ballotti ◽  
G. Bernardi
Keyword(s):  
Guinea Pig ◽  
Drug Induced ◽  
Pig Brain ◽  
Induced Changes ◽  
Guinea Pig Brain

Effect of (-)nicotine on GABA efflux from guinea pig cerebral cortex slices

1989 ◽  
Vol 21 ◽  
pp. 133-134 ◽  
Author(s):  
P. Spalluto ◽  
M. Morari ◽  
L. Ferraro ◽  
A. Nordberg ◽  
T. Antonelli ◽  
...  
Keyword(s):  
Cerebral Cortex ◽  
Guinea Pig ◽  
Cortex Slices

Changes in the Cortex Neurons in Single and Fractional Gamma Radiation

2021 ◽  
Vol 66 (4) ◽  
pp. 18-24
Author(s):  
I. Ushakov ◽  
Vladimir Fyodorov
Keyword(s):  
Nervous System ◽  
Cerebral Cortex ◽  
Radiation Exposure ◽  
Comparative Assessment ◽  
Male Rats ◽  
Fractionated Irradiation ◽  
Radiation Induced ◽  
Post Radiation ◽  
Induced Changes ◽  
The Brain

Purpose: Comparative assessment of radiation-induced changes in neurons of the cerebral cortex after a single and fractionated exposure to ionizing radiation in doses of 0.1 – 1.0 Gy. Material and methods. The study was carried out in compliance with the rules of bioethics on 180 white outbred male rats at the age of 4 months. by the beginning of the experiment, exposed to a single or fractionated exposure to γ-quanta of 60Co in total doses of 0.1; 0.2; 0.5 and 1.0 Gy. Neuromorphological and histochemical methods were used to assess morphometric and tinctorial parameters of nerve cells, as well as changes in the content of protein and nucleic acids in neurons in the early and late periods of the post-radiation period. Using one-way analysis of variance, a comparative assessment of neuromorphological indicators under various modes of radiation exposure is given. Results: In the control and irradiated animals throughout their life, undulating changes in the indicators of the state of the neurons of the brain occur with a gradual decrease by the end of the experiment. Despite a number of features of the dynamics of neuromorphological parameters, these irradiation regimes do not cause functionally significant changes in the neurons of the cortex. However, in some periods of the post-radiation period, the changes under the studied irradiation regimes were multidirectional and did not always correspond to age control. Significant differences in the response of neurons to these modes of radiation exposure in the sensory and motor areas of the cerebral cortex have not been established. Conclusion: No functionally significant radiation-induced changes in neurons were found either with single or fractionated irradiation. At the same time, different modes of irradiation in general caused the same type of changes in neurons. However, in some periods of observation, changes in neuromorphological parameters under the studied irradiation regimes were not unidirectional and differed from age control, which indicates a possible risk of disturbances in the functioning of the nervous system against the background of other harmful and dangerous factors.

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