Properties of Middle-Repeat Sequences in Nuclear Deoxyribonucleic Acid from Baby-Hamster Kidney Cells (BHK-21/C13)

1979 ◽  
Vol 7 (4) ◽  
pp. 663-665
Author(s):  
ALAN McLACHLAN ◽  
DAVID A. F. GILLESPIE ◽  
NORMAN HARDMAN
Keyword(s):  
Deoxyribonucleic Acid ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Repeat Sequences ◽  
Nuclear Deoxyribonucleic Acid ◽  
Baby Hamster Kidney Cells

scholarly journals Synthesis of deoxyribonucleic acid in BHK-21/C13 cells

1973 ◽  
Vol 131 (3) ◽  
pp. 499-508 ◽  
Author(s):  
Geoffrey J. Hayton ◽  
Colin K. Pearson ◽  
Jeremy R. Scaife ◽  
Hamish M. Keir
Keyword(s):  
Deoxyribonucleic Acid ◽  
Density Gradient Centrifugation ◽  
Gradient Centrifugation ◽  
Deae Cellulose ◽  
Sucrose Density Gradient ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Sucrose Density Gradient Centrifugation ◽  
Mechanism Model ◽  
Baby Hamster Kidney Cells

Newly synthesized 3H-labelled DNA was extracted from baby hamster kidney cells (BHK-21/C13 cells) and was shown to possess single-stranded properties when examined by column chromatography on benzoylated naphthoylated DEAE-cellulose, hydroxyapatite and methylated albumin on kieselguhr, and by its affinity for nitrocellulose filters. Some of the newly synthesized DNA was shown to be of lower molecular weight than the bulk of the DNA when examined by alkaline sucrose-density-gradient centrifugation. The properties observed were not affected by treatment of the DNA with ribonuclease, Pronase or amylase. The effect of the size of the DNA on its observed properties was examined and is discussed. It is concluded that DNA synthesis in BHK-21/C13 cells proceeds according to the discontinuous-mechanism model in at least one of the strands.

Turbomolecular Pumped Electron Microscopy of Rubella Virus (In BHK Celis)

1968 ◽  
Vol 26 ◽  
pp. 204-205
Author(s):  
A. B. Taylor ◽  
G. C. Cole ◽  
M. A. Holcomb ◽  
C. A. Baechler
Keyword(s):  
Electron Microscopy ◽  
Rubella Virus ◽  
Phosphate Buffer ◽  
Fetal Calf Serum ◽  
Calf Serum ◽  
Basal Medium ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Input Multiplicity ◽  
Baby Hamster Kidney Cells

An aliquot from a continuous fermenter culture of baby hamster kidney cells (BHK-21 Clone PD-4) (Wistar) maintained in Ca free Eagle's Basal Medium containing 2% Kaolin adsorbed fetal calf serum was planted in spinner flasks at 300,000 cells per ml, total volume 600 ml. After equilibration for one day at 35°C to insure that cells were in log phase, the culture was infected with the M-33-AGMK25 BHK-219 strain of rubella at an input multiplicity of about 6 TCID50 per cell. The virus was identified with specific rubella antiserum.Preliminary experiments had shown that such cultures would reach a peak or plateau HA titer of approximately 1:64, 24 hrs after inoculation and would continue to yield virus for 6 to 12 days. One hundred ml aliquot harvests were withdrawn daily and the culture was returned to volume with growth medium and incubation continued. The harvested cells were spun down rapidly at 2500 rpm per 15 mins., fixed in 3.7% gluteraldehyde in Ca free phosphate buffer saline, and post fixed in osmium tetraoxide. After dehydration, the cells were embedded in Epon 812 and cured approximately 20 hrs at 60°C.

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