Effect of aloe lectin on deoxyribonucleic acid synthesis in baby hamster kidney cells

1985 ◽  
Vol 41 (5) ◽  
pp. 669-671 ◽  
Author(s):  
A. Yagi ◽  
K. Machii ◽  
H. Nishimura ◽  
T. Shida ◽  
I. Nishioka
Keyword(s):  
Deoxyribonucleic Acid ◽  
Acid Synthesis ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Deoxyribonucleic Acid Synthesis ◽  
Baby Hamster Kidney Cells

scholarly journals Synthesis of deoxyribonucleic acid in BHK-21/C13 cells

1973 ◽  
Vol 131 (3) ◽  
pp. 499-508 ◽  
Author(s):  
Geoffrey J. Hayton ◽  
Colin K. Pearson ◽  
Jeremy R. Scaife ◽  
Hamish M. Keir
Keyword(s):  
Deoxyribonucleic Acid ◽  
Density Gradient Centrifugation ◽  
Gradient Centrifugation ◽  
Deae Cellulose ◽  
Sucrose Density Gradient ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Sucrose Density Gradient Centrifugation ◽  
Mechanism Model ◽  
Baby Hamster Kidney Cells

Newly synthesized 3H-labelled DNA was extracted from baby hamster kidney cells (BHK-21/C13 cells) and was shown to possess single-stranded properties when examined by column chromatography on benzoylated naphthoylated DEAE-cellulose, hydroxyapatite and methylated albumin on kieselguhr, and by its affinity for nitrocellulose filters. Some of the newly synthesized DNA was shown to be of lower molecular weight than the bulk of the DNA when examined by alkaline sucrose-density-gradient centrifugation. The properties observed were not affected by treatment of the DNA with ribonuclease, Pronase or amylase. The effect of the size of the DNA on its observed properties was examined and is discussed. It is concluded that DNA synthesis in BHK-21/C13 cells proceeds according to the discontinuous-mechanism model in at least one of the strands.

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