The Metabolism of Chemically Modified Insulins

1978 ◽  
Vol 6 (3) ◽  
pp. 555-557
Author(s):  
D. PAPACHRISTODOULOU ◽  
D. BRANDENBURG ◽  
D. I. DRON ◽  
R. H. JONES ◽  
P. H. SÖNKSEN ◽  
...  
Keyword(s):  
Chemically Modified ◽  
Chemically Modified Insulins

scholarly journals Receptor-binding assay of chemically modified insulins

Diabetologia ◽  
1974 ◽  
Vol 10 (1) ◽  
pp. 1-5 ◽  
Author(s):  
P. Freychet ◽  
D. Brandenburg ◽  
A. Wollmer
Keyword(s):  
Receptor Binding ◽  
Binding Assay ◽  
Chemically Modified ◽  
Receptor Binding Assay ◽  
Chemically Modified Insulins

scholarly journals Insulin action on adipocytes. Evidence that the anti-lipolytic and lipogenic effects of insulin are mediated by the same receptor

1979 ◽  
Vol 184 (2) ◽  
pp. 355-360 ◽  
Author(s):  
S H L Thomas ◽  
M H Wisher ◽  
D Brandenburg ◽  
P H Sönksen
Keyword(s):  
Dose Response ◽  
Insulin Action ◽  
Insulin Analogues ◽  
Bovine Insulin ◽  
Maximal Effect ◽  
Maximal Concentration ◽  
Insulin Stimulation ◽  
Chemically Modified ◽  
Chemically Modified Insulins ◽  
Stimulation Of

1. The dose-response relationships of insulin stimulation of lipogenesis and inhibition of lipolysis were studied simultaneously by using rat adipocytes to determine whether these different effects of insulin are mediated through the same or different sets of receptors. 2. The sensitivity (defined as the concentration of insulin required to produce a half-maximal effect) of the stimulated lipogenic response to insulin was not significantly different from the sensitivity of the anti-lipolytic response to insulin. The addition of different adrenaline and glucose concentrations did not alter the half-maximal concentration of insulin required to inhibit lipolysis. 3. The specificities of the lipogenic and antilipolytic responses were studied by using insulin analogues. The sensitivities of the lipogenic and anti-lipolytic responses were the same for five chemically modified insulins and hagfish insulin, which have potencies compared with bovine insulin of between 3 and 90%. 4. Starving rats for 48h significantly increased the sensitivities of both the antilipolytic and lipogenic responses to insulin, but the changes in the sensitivities of both lipogenesis and anti-lipolysis returned to that of fed rats. 5. We conclude that insulin stimulates lipogenesis and inhibits lipolysis over the same concentration range. These observations provide powerful evidence that the different effects of insulin are mediated through the same set of receptors.

High‐performance reversed‐phase flash chromatography purification of peptides and chemically modified insulins

ChemBioChem ◽  
2021 ◽  
Author(s):  
Kasper K Sørensen ◽  
Narendra Kumar Mishra ◽  
Maciej P Paprocki ◽  
Amit Mehrotra ◽  
Knud J. Jensen
Keyword(s):  
High Performance ◽  
Reversed Phase ◽  
Flash Chromatography ◽  
Chemically Modified ◽  
Chemically Modified Insulins

scholarly journals Biological properties of chemically modified insulins. I. Biological activity of proinsulin and insulin modified at A1-glycine and B29-lysine

Diabetologia ◽  
1976 ◽  
Vol 12 (6) ◽  
pp. 601-608 ◽  
Author(s):  
R. H. Jones ◽  
D. I. Dron ◽  
M. J. Ellis ◽  
P. H. S�nksen ◽  
D. Brandenburg
Keyword(s):  
Biological Activity ◽  
Biological Properties ◽  
Chemically Modified ◽  
Chemically Modified Insulins

Radioimmunoassay of Chemically-Modified Insulins - Use and Misuse

1980 ◽  
pp. 619-626
Author(s):  
J.H. Thomas ◽  
D.I. Dron ◽  
R.H. Jones ◽  
P.H. Sönksen ◽  
D. Brandenburg
Keyword(s):  
Chemically Modified ◽  
Chemically Modified Insulins

Enzymatic and hyperglycemia stability of chemically modified insulins with hydrophobic acyl groups

2004 ◽  
Vol 14 (2) ◽  
pp. 481-483 ◽  
Author(s):  
Kentaro Nakashima ◽  
Makoto Miyagi ◽  
Koichi Goto ◽  
Yoko Matsumoto ◽  
Ryuichi Ueoka
Keyword(s):  
Chemically Modified ◽  
Chemically Modified Insulins

scholarly journals Radioimmunoassay of chemically modified insulins

Diabetologia ◽  
1980 ◽  
Vol 18 (1) ◽  
pp. 59-63 ◽  
Author(s):  
D. I. Dron ◽  
R. H. Jones ◽  
P. H. S�nksen ◽  
J. H. Thomas ◽  
D. Brandenburg
Keyword(s):  
Chemically Modified ◽  
Chemically Modified Insulins

Binding of Tissue Plasminogen Activator to Vascular Grafts

1989 ◽  
Vol 61 (01) ◽  
pp. 131-136 ◽  
Author(s):  
Richard A Harvey ◽  
Hugh C Kim ◽  
Jonathan Pincus ◽  
Stanley Z Trooskin ◽  
Josiah N Wilcox ◽  
...  
Keyword(s):  
Plasminogen Activator ◽  
Tissue Plasminogen Activator ◽  
Fibrinolytic Activity ◽  
Polymer Surface ◽  
Enzymic Activity ◽  
Vascular Prostheses ◽  
Chemically Modified ◽  
Insoluble Surfactant ◽  
Tissue Plasminogen

SummaryTissue plasminogen activator labeled with radioactive iodine (125I-tPA) was immobilized on vascular prostheses chemically modified with a thin coating of water-insoluble surfactant, tridodecylmethylammonium chloride (TDM AC). Surfactant- treated Dacron, polytetrafluoroethylene (PTFE), silastic, polyethylene and polyurethane bound appreciable amounts of 125I- tPA (5-30 μg 125I-tPA/cm2). Upon exposure to human plasma, the amount of 125I-tPA bound to the surface shows an initial drop during the first hour of incubation, followed by a slower, roughly exponential release with a t½ of appoximately 75 hours. Prostheses containing bound tPA show fibrinolytic activity as measured both by lysis of clots formed in vitro, and by hydrolysis of a synthetic polypeptide substrate. Prior to incubation in plasma, tPA bound to a polymer surface has an enzymic activity similar, if not identical to that of the native enzyme in buffered solution. However, exposure to plasma causes a decrease in the fibrinolytic activity of both bound tPA and enzyme released from the surface of the polymer. These data demonstrate that surfactant-treated prostheses can bind tPA, and that these chemically modified devices can act as a slow-release drug delivery system with the potential for reducing prosthesis-induced thromboembolism.

Sign in / Sign up

Export Citation Format

Share Document