High‐performance reversed‐phase flash chromatography purification of peptides and chemically modified insulins

ChemBioChem ◽  
2021 ◽  
Author(s):  
Kasper K Sørensen ◽  
Narendra Kumar Mishra ◽  
Maciej P Paprocki ◽  
Amit Mehrotra ◽  
Knud J. Jensen
Keyword(s):  
High Performance ◽  
Reversed Phase ◽  
Flash Chromatography ◽  
Chemically Modified ◽  
Chemically Modified Insulins

An endogenous digitalis-like compound extracted from human urine: biochemical and chemical studies

1987 ◽  
Vol 65 (8) ◽  
pp. 1522-1527 ◽  
Author(s):  
Jean-François Cloix ◽  
Maryse Crabos ◽  
Marie-Laure Grichois ◽  
Philippe Meyer
Keyword(s):  
High Performance ◽  
Anion Exchange Chromatography ◽  
Reversed Phase ◽  
Human Platelets ◽  
Exchange Chromatography ◽  
Flash Chromatography ◽  
Experimental Hypertension ◽  
Hypertensive Patients ◽  
Chemical Studies ◽  
History Of

Plasma and urine levels of an endogenous digitalis-like compound (EDLC) are increased in low renin Na+-dependent experimental hypertension, in some normotensive offspring of hypertensive patients and in some essential hypertensive patients. Urine-drived EDLC was purified from 550 L of urine from essential hypertensive patients (n = 8) and from normotensive subjects with a family history of hypertension (n = 27), using flash chromatography on C18 reversed-phase, anion exchange chromatography and various reversed-phase high performance liquid chromatographies. The mechanism of Na+–K+ ATPase inhibition and the related effects of semipurified urine-derived EDLC were studied and compared with those of ouabain. Its action was similar to that of ouabain in 8 out of 10 of the tests applied. The main effects of such a compound were the depression of Na+–K+ pump activity of human erythrocytes, the inhibition of 5-hydroxytryptamine reuptake by human platelets, and the induction of natriuresis in urethanized rats. Therefore, EDLC may be considered as one of the natriuretic hormones whose mechanism of action closely resembles that of ouabain.

Development and Validation of A Reversed Phase-High Performance Liquid Chromatography Method for the Quantitative Estimation of Ramipril Drug Content from Formulated Dosage Form

2016 ◽  
Vol 6 (3) ◽  
Author(s):  
Raju Chandra ◽  
Manisha Pant ◽  
Harchan Singh ◽  
Deepak Kumar ◽  
Ashwani Sanghi
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Active Ingredient ◽  
High Performance ◽  
Limit Of Detection ◽  
Quantitative Estimation ◽  
Reversed Phase ◽  
Uv Detector ◽  
Chromatography Method ◽  
Drug Content

A reliable and reproducible reversed-phase high performance liquid chromatography (RP-HPLC) was developed for the quantitative determination of Remipril drug content from marketed bulk tablets. The active ingredient of Remipril separation achieved with C18 column using the methanol water mobile phase in the ratio of 40:60 (v/v). The active ingredient of the drug content quantify with UV detector at 215 nm. The retention time of Remipril is 5.63 min. A good linearity relation (R2=0.999) was obtained between drug concentration and average peak areas. The limit of detection and limit of quantification of the instrument were calculated 0.03 and 0.09 µg/mL, respectively. The accuracy of the method validation was determined 102.72% by recoveries method.

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