Isolation and Characterization of Respiratory-Deficient Mutants of Kluyveromyces lactis, a perite-Negative Yeast

1977 ◽  
Vol 5 (5) ◽  
pp. 1498-1500 ◽  
Author(s):  
BARBARA M. ALLMARK ◽  
SUSAN M. DANKS ◽  
PETER A. WHITTAKER
Keyword(s):  
Kluyveromyces Lactis ◽  
Isolation And Characterization ◽  
Respiratory Deficient

Isolation and characterization of mutants of Kluyveromyces lactis defective in lactose transport

1987 ◽  
Vol 208 (1-2) ◽  
pp. 145-151 ◽  
Author(s):  
Michael I. Riley ◽  
K. Sreekrishna ◽  
Srirama Bhairi ◽  
Robert C. Dickson
Keyword(s):  
Kluyveromyces Lactis ◽  
Isolation And Characterization ◽  
Lactose Transport

Isolation and Characterization of Mutants of Saccharomyces cerevisiae Auxotrophic and Conditionally Auxotrophic for 5′ -dTMP

1974 ◽  
Vol 29 (11-12) ◽  
pp. 733-738 ◽  
Author(s):  
Martin Brendel ◽  
Wolfgang W. Fäth
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Complementation Group ◽  
Improved Method ◽  
Genetical Analysis ◽  
Type Locus ◽  
Isolation And Characterization ◽  
Folic Acid Antagonists ◽  
I Gene ◽  
Respiratory Deficient

Abstract An improved method for isolation of yeast m utants auxotrophic for 5′-dTM P is presented. The procedure employs the two folic acid antagonists am inopterin and sulfanilam ide (SAA). Selectiveness of the procedure depends on concentration of SAA and time of incubation. 44 mutants auxotrophic and 3 conditionally auxotrophic for 5′-dTMP were isolated. All belong to one complementation group. The corresponding gene was designated TMP1. Tetrad dissection revealed its chromosomal nature. TMP1 is not closely linked to the genes ADE2,, LEU1, ARG 4, ILV2, HIS5, LYS1 and the mating type locus. With the centromere-linked genes ARG4 and LEU1 I gene TMP1 exhibited second division segregation frequencies of 0.42 and 0.53 respectively, indicative of centromere-linkage. Strains auxotrophic and conditionally auxotrophic for 5′-dTM P were all respiratory deficient (petite). Genetical analysis indicates that the petite phenotype is due to loss of the rho factor in cells harbouring either tmp1 or tmp1ts alleles.

Isolation and characterization of the RAD59 homologue of Kluyveromyces lactis

2001 ◽  
Vol 39 (5-6) ◽  
pp. 305-310 ◽  
Author(s):  
Michael van den Bosch ◽  
Jos� B. Zonneveld ◽  
Paul H. Lohman ◽  
Albert Pastink
Keyword(s):  
Kluyveromyces Lactis ◽  
Isolation And Characterization

Isolation and characterization of the gene encoding xylose reductase from Kluyveromyces lactis

Gene ◽  
1995 ◽  
Vol 162 (1) ◽  
pp. 93-97 ◽  
Author(s):  
Patrick Billard ◽  
Sandrine Ménart ◽  
Reinhard Fleer ◽  
Monique Bolotin-Fukuhara
Keyword(s):  
Kluyveromyces Lactis ◽  
Xylose Reductase ◽  
Gene Encoding ◽  
Isolation And Characterization

Isolation and characterization of mutants of Saccharomyces cerevisiae Resistant to Killer Toxin of Kluyveromyces lactis

1990 ◽  
Vol 70 (4) ◽  
pp. 222-227 ◽  
Author(s):  
Shinichi Kawamoto ◽  
Naomi Arai ◽  
Masaaki Kobayashi ◽  
Kazumitsu Kawahara ◽  
Hitoshi Iwahashi ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Kluyveromyces Lactis ◽  
Killer Toxin ◽  
Isolation And Characterization
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