Turkey Liver Xanthine Dehydrogenase: Effects of Methanol on the Enzyme-Catalysed Oxidation of Reduced Nicotinamide-Adenine Dinucleotide

1976 ◽  
Vol 4 (5) ◽  
pp. 901-903 ◽  
Author(s):  
ÍDE NÍ FHAOLÁIN ◽  
MICHAEL J. HYNES ◽  
MICHAEL P. COUGHLAN
Keyword(s):  
Nicotinamide Adenine Dinucleotide ◽  
Xanthine Dehydrogenase ◽  
Nicotinamide Adenine ◽  
Catalysed Oxidation ◽  
Reduced Nicotinamide Adenine Dinucleotide

scholarly journals Turkey liver xanthine dehydrogenase. Relation between nicotinamide-adenine dinucleotide oxidoreductase activity and the content of functional enzyme

1976 ◽  
Vol 157 (1) ◽  
pp. 283-285 ◽  
Author(s):  
I N Fhaoláin ◽  
M P Coughlan
Keyword(s):  
Methylene Blue ◽  
Nicotinamide Adenine Dinucleotide ◽  
Xanthine Dehydrogenase ◽  
Nicotinamide Adenine ◽  
Active Centre ◽  
Oxidoreductase Activity ◽  
Catalysed Oxidation

The turkey liver xanthine dehydrogenase-catalysed oxidation of NADH by Methylene Blue, by ferricyanide or by O2 is not dependent on the integrity of the active-centre persulphide groups. By contrast, the NADH-dichlorophenol-indophenol oxidoreductase and NADH-trinitrobenzenesulphonate oxidoreductase activities are directly proportional to the content of functional enzyme. These findings help to identify the sites of egress of electrons to oxidizing substrates.

scholarly journals The regulation of xanthine oxidase. Inhibition by reduced nicotinamide–adenine dinucleotide of rat liver xanthine oxidase type D and of chick liver xanthine dehydrogenase

1970 ◽  
Vol 117 (1) ◽  
pp. 97-100 ◽  
Author(s):  
E. Della Corte ◽  
F. Stirpe
Keyword(s):  
Xanthine Oxidase ◽  
Rat Liver ◽  
Nicotinamide Adenine Dinucleotide ◽  
Enzyme Activities ◽  
Xanthine Dehydrogenase ◽  
Nicotinamide Adenine ◽  
Type D ◽  
Oxidase Inhibition ◽  
Xanthine Oxidase Inhibition ◽  
Reduced Nicotinamide Adenine Dinucleotide

1. Rat liver xanthine oxidase type D (NAD+-dependent) and chick liver xanthine oxidase are inhibited by NADH, which competes with NAD+. 2. The addition of a NADH-reoxidizing system in the assay of these enzyme activities is proposed. 3. Rat liver xanthine oxidase type O (oxygen-dependent) is not affected by NADH.

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