(Sodium plus Potassium Ion)-Dependent Adenosine Triphosphatase Activity in Reconstituted Systems and in Cultured Cells

1976 ◽  
Vol 4 (4) ◽  
pp. 755-757 ◽  
Author(s):  
HAROLD K. KIMELBERG
Keyword(s):  
Adenosine Triphosphatase ◽  
Cultured Cells ◽  
Potassium Ion ◽  
Adenosine Triphosphatase Activity

scholarly journals LOCALIZATION OF MITOCHONDRIAL ADENOSINE TRIPHOSPHATASE ACTIVITY IN CULTURED HUMAN CELLS

1965 ◽  
Vol 13 (8) ◽  
pp. 647-656 ◽  
Author(s):  
EDWARD ESSNER ◽  
JØRGEN FOGH ◽  
PATRICIA FABRIZIO
Keyword(s):  
Electron Microscopy ◽  
Mitochondrial Membrane ◽  
Adenosine Triphosphatase ◽  
Cultured Cells ◽  
Enzyme Reaction ◽  
Outer Mitochondrial Membrane ◽  
Adenosine Triphosphatase Activity ◽  
Different Types ◽  
Cultured Human Cells ◽  
Membrane Reaction

Adenosine triphosphatase activity has been localized by light microscopy in the mitochondria of four different types of cultured cells using the Wachstein-Meisel lead method after brief fixation in formol-calcium. The resolutions of the method permits the study of mitochondrial size, form, number and distributions in these cultured cells. Electron microscopy shows enzyme reaction product within the mitochondrion but not on the outer mitochondrial membrane. Reaction product is also localized to the plasma membrane and its infoldings.

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