scholarly journals Microstructure and mechanical properties of subchondral bone are negatively regulated by tramadol in osteoarthritis in mice

2020 ◽  
Vol 40 (9) ◽  
Author(s):  
Chen-Chen Ji ◽  
Bo Liu ◽  
Yi-Jie Shao ◽  
Ting Liang ◽  
Hua-Ye Jiang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Subchondral Bone ◽  
Volume Fraction ◽  
Ct Scanning ◽  
Knee Joints ◽  
Micro Computed Tomography ◽  
Bone Volume Fraction ◽  
Treatment Groups ◽  
Microstructure And Mechanical Properties ◽  
Histological Staining

Abstract Objectives: In the treatment of osteoarthritis (OA), tramadol, a common weak opioid, has become popular due to its effectiveness in inhibition of pain. In the present study, we aimed to explore the effect of tramadol on subchondral bone, especially changes in the microstructure and mechanical properties. Methods: A mouse model of OA was established in the present study by destabilization of the medial meniscus (DMM). A vehicle or drug was administered for 4 weeks. Specimens were harvested and analyzed radiologically and histologically using micro-computed tomography (micro-CT), scanning electron microscopy (SEM), atomic force microscopy (AFM) and histological staining to evaluate the knee joints of mice undergoing different forms of intervention. Results: In the early stages of OA induced by DMM, the subchondral bone volume fraction in the OA group was significantly higher than in the sham+vehicle (sham+veh) group, while the volume in the treatment groups was lower than in the DMM+vehicle (DMM+veh) and sham+veh groups. In addition, the elastic moduli in the treatment groups clearly decreased compared with the DMM+veh and sham+veh groups. Observations of the subchondral bone surface by SEM indicated serious destruction, principally manifesting as a decrease in lacunae and more numerous and scattered cracks. Histological staining demonstrated that there was no difference in the degeneration of either the articular cartilage or synovial cells whether tramadol was used or not. Conclusion: Although tramadol is effective in inhibiting pain in early OA, it negatively regulates the microstructure and mechanical properties of subchondral bone in joints.

scholarly journals Structure-Function Relationships of the Vertebral Endplate

2021 ◽  
Author(s):  
Yuanqiao Wu ◽  
Elise Feng-i Morgan ◽  
Johnfredy Loaiza ◽  
Rohin Banerji ◽  
Olivia Rose Blouin
Keyword(s):  
Mechanical Properties ◽  
Mechanical Behavior ◽  
Yield Stress ◽  
Volume Fraction ◽  
Level Density ◽  
Micro Computed Tomography ◽  
Vertebral Endplate ◽  
Mineral Density ◽  
Bone Volume Fraction ◽  
Apparent Stiffness

Background: Although deformation and fracture of the vertebral endplate have been implicated in spinal conditions such as vertebral fracture and disc degeneration, few biomechanical studies of this structure are available. The goal of this study was to quantify the mechanical behavior of the vertebral endplate. Methods: Eight-five rectangular specimens were dissected from the superior and/or inferior central endplates of human lumbar spine segments L1-L4. Micro-computed tomography (μCT) imaging, four-point-bend testing, and ashing were performed to quantify the apparent elastic modulus and yield stress (modulus and yield stress, respectively, of the porous vertebral endplate), tissue yield stress (yield stress of the tissue of the vertebral endplate, excluding pores), ultimate strain, fracture strain, bone volume fraction (BV/TV), bone mineral density (BMD), and various measures of tissue density and composition (tissue mineral density, ash fraction, and ash density). Regression was used to assess the dependence of mechanical properties on density and composition. Results: Wide variations in elastic and failure properties, and in density and tissue composition, were observed. BMD and BV/TV were good predictors of many of the apparent-level mechanical properties, including modulus, yield stress, and in the case of the inferior vertebral endplate, failure strains. Similar values of the mechanical properties were noted between superior and inferior vertebral endplates. In contrast to the dependence of apparent stiffness and strength on BMD and BV/TV, none of the mechanical properties depended on any of the tissue-level density measurements. Conclusion: The dependence of many of the mechanical properties of the vertebral endplate on BV/TV and BMD suggests possibilities for non-invasive assessment of how this region of the spine behaves during habitual and injurious loading. Further study of the non-mineral components of the endplate tissue is required to understand how the composition of this tissue may influence the overall mechanical behavior of the vertebral endplate.

Prediction of Mechanical Properties of the Cancellous Bone of the Mandibular Condyle

2003 ◽  
Vol 82 (10) ◽  
pp. 819-823 ◽  
Author(s):  
L.J. van Ruijven ◽  
E.B.W. Giesen ◽  
M. Farella ◽  
T.M.G.J. van Eijden
Keyword(s):  
Mechanical Properties ◽  
Cancellous Bone ◽  
Volume Fraction ◽  
Bone Structure ◽  
Structural Parameters ◽  
Mandibular Condyle ◽  
Element Model ◽  
Micro Computed Tomography ◽  
Bone Volume Fraction ◽  
Apparent Stiffness

The mechanical properties of cancellous bone depend on the bone structure. The present study examined the extent to which the apparent stiffness of the cancellous bone of the human mandibular condyle can be predicted from its structure. Two models were compared. The first, a structure model, used structural parameters such as bone volume fraction and anisotropy to estimate the apparent stiffness. The second was a finite element model (FEM) of the cancellous bone. The bone structure was characterized by micro-computed tomography. The calculated stiffnesses of 24 bone samples were compared with measured stiffnesses. Both models could predict 89% of the variation in the measured stiffnesses. From the stiffness approximated by FEM in combination with the measured stiffness, the stiffness of the bone tissue was estimated to be 11.1 ± 3.2 GPa. It was concluded that both models could predict the stiffness of cancellous bone with adequate accuracy.

Contributions of Trabecular Rods of Various Orientations in Determining the Elastic Properties of Human Vertebral Trabecular Bone

2007 ◽  
Author(s):  
Xiaowei S. Liu ◽  
X. Henry Zhang ◽  
Paul Sajda ◽  
Punam K. Saha ◽  
Felix W. Wehrli ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Trabecular Bone ◽  
Elastic Properties ◽  
Volume Fraction ◽  
Three Dimensional ◽  
Micro Computed Tomography ◽  
Bone Volume Fraction ◽  
Human Trabecular Bone ◽  
Vertebral Trabecular Bone ◽  
Age Related

Osteoporosis is an age-related disease characterized by low bone mass and architectural deterioration. Other than bone volume fraction (BV/TV), microarchitecture of trabecular bone, such as trabecular type (rods or plates), connectivity, and orientation of the trabecular network is also believed to be important in governing the mechanical properties of trabecular bone. A recent study [1] showed that the microarchitecture alone affects elastic moduli of trabecular bone and, further, that trabecular plates make a far greater contribution than rods. In human vertebral trabecular bone, the roles of transverse vs. vertical rods in conferring mechanical properties of trabecular bone have been debated [2, 3]. It has been suggested that the role of transverse trabecular rod is critical in determining elastic modulus of vertebral trabecular bone. However, without explicit classifications of trabecular type, or orientation assessment at an individual trabecula level, it is not possible yet to test this hypothesis in human trabecular bone samples despite the development of three-dimensional (3D) micro computed tomography (μCT) and μCT based finite element (FE) models of human trabecular bone. With the newly developed technique of complete volumetric decomposition and individual trabecula based orientation analyses [4], now it is possible to quantitatively examine the contributions of trabecular rods of various orientations in the elastic properties of vertebral trabecular bone.

Changed Morphology and Mechanical Properties of Cancellous Bone in the Mandibular Condyles of Edentate People

2004 ◽  
Vol 83 (3) ◽  
pp. 255-259 ◽  
Author(s):  
E.B.W. Giesen ◽  
M. Ding ◽  
M. Dalstra ◽  
T.M.G.J. van Eijden
Keyword(s):  
Mechanical Properties ◽  
Cancellous Bone ◽  
Regression Models ◽  
Volume Fraction ◽  
Mechanical Load ◽  
Micro Computed Tomography ◽  
Bone Morphology ◽  
Bone Volume Fraction ◽  
Fundamental Relationship ◽  
Morphology And Mechanical Properties

Since edentate subjects have a reduced masticatory function, it can be expected that the morphology of the cancellous bone of their mandibular condyles has changed according to the altered mechanical environment. In the present study, the morphology of cylindrical cancellous bone specimens of the mandibular condyles of edentate subjects (n = 25) was compared with that of dentate subjects (n = 24) by means of micro-computed tomography and by the application of Archimedes’ principle. Stiffness and strength were determined by destructive mechanical testing. Compared with dentate subjects, it appeared that, in edentate subjects, the bone was less dense and the trabecular structure was less plate-like. The regression models of stiffness and strength built from bone volume fraction and the trabecular orientation relative to the axis of the specimen were similar for both dentate and edentate subjects. This indicates that, under reduced mechanical load, the fundamental relationship between bone morphology and mechanical properties does not change.

scholarly journals Etoricoxib decreases mouse subchondral bone mass and biomechanical properties in early osteoarthritis

2020 ◽  
Author(s):  
Bo Liu ◽  
Chen-Chen Ji ◽  
Yi-Jie Shao ◽  
Ting Liang ◽  
Jia-Heng He ◽  
...  
Keyword(s):  
Elastic Modulus ◽  
Subchondral Bone ◽  
Structural Changes ◽  
Sham Group ◽  
Volume Fraction ◽  
Control Group ◽  
Micro Computed Tomography ◽  
Bone Volume Fraction ◽  
Trabecular Thickness ◽  
Early Oa

Abstract BackgroundEtoricoxib, a selective Cyclooxygenase-2 (COX-2) inhibitor, is commonly used in osteoarthritis (OA) for pain relief. The purpose of our study was to investigate the effects of Etoricoxib on mouse subchondral bone in early OA.MethodsOA was induced via destabilization of the medial meniscus (DMM) in C57BL/6J mice. After surgery, the mice were randomly and equally divided into five groups: a sham-operated control group (Sham group), an osteoarthritis (OA) group (DMM group), an OA treated with Etoricoxib 5mg/kg (DMM+E5) group, an OA treated with Etoricoxib 10mg/kg (DMM+E10) group, and an OA treated with Etoricoxib 20mg/kg (DMM+E20) group. Mice in the Sham group and DMM group were injected with a similar dose of vehicle (40% ethyl alcohol–saline solution). Four weeks after treatment, mice were euthanized. Micro computed tomography (Mirco-CT) analysis, Safranin O-Fast Green staining, hematoxylin and eosin (HE) staining were performed to evaluate morphological and structural changes. In addition, atomic force microscopy (AFM) analysis was performed to evaluate changes in the elastic modulus. Furthermore, changes in microstructure were detected by scanning electron microscopy (SEM).ResultsEtoricoxib inhibited osteophyte formation in the subchondral bone. However, it also reduced the bone volume fraction (BV/TV), lowered trabecular thickness (Tb.Th), and more microfractures and pores were observed in the subchondral bone. Moreover, Etoricoxib reduced the elastic modulus of subchondral bone. Furthermore, exposure to Etoricoxib further increased the empty/total osteocyte ratio of the subchondral bone. In cartilage and synovium, Etoricoxib did not significantly change the Osteoarthritis Research Society International (OARSI) score, the modified Mankin score, and the synovialitis-score versus the DMM group. ConclusionOur results demonstrate that although Etoricoxib can relieve the pain induced by OA, it also has adverse effects on subchondral bone in early OA.

scholarly journals The Effect of Quenching and Partitioning (Q&P) Heat Treatment on the Microstructure and Mechanical Properties of High Boron Steel

Materials ◽  
2021 ◽  
Vol 14 (6) ◽  
pp. 1556
Author(s):  
Zhao Li ◽  
Run Wu ◽  
Mingwei Li ◽  
Song-Sheng Zeng ◽  
Yu Wang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Heat Treatment ◽  
Volume Fraction ◽  
Martensitic Steel ◽  
Boron Steel ◽  
Quenching And Partitioning ◽  
Effect Mechanism ◽  
Microstructure And Mechanical Properties ◽  
High Boron ◽  
Cast Condition

High boron steel is prone to brittle failure due to the boride distributed in it with net-like or fishbone morphology, which limit its applications. The Quenching and Partitioning (Q&P) heat treatment is a promising process to produce martensitic steel with excellent mechanical properties, especially high toughness by increasing the volume fraction of retained austensite (RA) in the martensitic matrix. In this work, the Q&P heat treatment is used to improve the inherent defect of insufficient toughness of high boron steel, and the effect mechanism of this process on microstructure transformation and the change of mechanical properties of the steel has also been investigated. The high boron steel as-casted is composed of martensite, retained austensite (RA) and eutectic borides. A proper quenching and partitioning heat treatment leads to a significant change of the microstructure and mechanical properties of the steel. The net-like and fishbone-like boride is partially broken and spheroidized. The volume fraction of RA increases from 10% in the as-cast condition to 19%, and its morphology also changes from blocky to film-like. Although the macro-hardness has slightly reduced, the toughness is significantly increased up to 7.5 J·cm−2, and the wear resistance is also improved.

scholarly journals Effect of Graphene Nanosheets Content on Microstructure and Mechanical Properties of Titanium Matrix Composite Produced by Cold Pressing and Sintering

Nanomaterials ◽  
2018 ◽  
Vol 8 (12) ◽  
pp. 1024 ◽  
Author(s):  
Milad Haghighi ◽  
Mohammad Shaeri ◽  
Arman Sedghi ◽  
Faramarz Djavanroodi
Keyword(s):  
Mechanical Properties ◽  
Matrix Composite ◽  
Volume Fraction ◽  
Graphene Nanosheets ◽  
Microstructural Analysis ◽  
Titanium Matrix ◽  
Titanium Matrix Composite ◽  
Cold Pressing ◽  
Sintering Time ◽  
Microstructure And Mechanical Properties

The effect of graphene nanosheet (GNS) reinforcement on the microstructure and mechanical properties of the titanium matrix composite has been discussed. For this purpose, composites with various GNS contents were prepared by cold pressing and sintering at various time periods. Density calculation by Archimedes’ principle revealed that Ti/GNSs composites with reasonable high density (more than 99.5% of theoretical density) were produced after sintering for 5 h. Microstructural analysis by X-ray diffraction (XRD) and a field emission scanning electron microscope (FESEM) showed that TiC particles were formed in the matrix during the sintering process as a result of a titanium reaction with carbon. Higher GNS content as well as sintering time resulted in an increase in TiC particle size and volume fraction. Microhardness and shear punch tests demonstrated considerable improvement of the specimens’ mechanical properties with the increment of sintering time and GNS content up to 1 wt. %. The microhardness and shear strength of 1 wt. % GNS composites were enhanced from 316 HV and 610 MPa to 613 HV and 754 MPa, respectively, when composites sintered for 5 h. It is worth mentioning that the formation of the agglomerates of unreacted GNSs in 1.5 wt. % GNS composites resulted in a dramatic decrease in mechanical properties.

scholarly journals Trabecular bone patterning in the hominoid distal femur

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5156 ◽  
Author(s):  
Leoni Georgiou ◽  
Tracy L. Kivell ◽  
Dieter H. Pahr ◽  
Matthew M. Skinner
Keyword(s):  
Trabecular Bone ◽  
Distal Femur ◽  
Volume Fraction ◽  
Reaction Force ◽  
Intraspecific Variability ◽  
Trabecular Structure ◽  
Trabecular Architecture ◽  
Micro Computed Tomography ◽  
Bone Volume Fraction ◽  
Distal Epiphysis

BackgroundIn addition to external bone shape and cortical bone thickness and distribution, the distribution and orientation of internal trabecular bone across individuals and species has yielded important functional information on how bone adapts in response to load. In particular, trabecular bone analysis has played a key role in studies of human and nonhuman primate locomotion and has shown that species with different locomotor repertoires display distinct trabecular architecture in various regions of the skeleton. In this study, we analyse trabecular structure throughout the distal femur of extant hominoids and test for differences due to locomotor loading regime.MethodsMicro-computed tomography scans ofHomo sapiens(n= 11),Pan troglodytes(n= 18),Gorilla gorilla(n= 14) andPongosp. (n= 7) were used to investigate trabecular structure throughout the distal epiphysis of the femur. We predicted that bone volume fraction (BV/TV) in the medial and lateral condyles inHomowould be distally concentrated and more anisotropic due to a habitual extended knee posture at the point of peak ground reaction force during bipedal locomotion, whereas great apes would show more posteriorly concentrated BV/TV and greater isotropy due to a flexed knee posture and more variable hindlimb use during locomotion.ResultsResults indicate some significant differences between taxa, with the most prominent being higher BV/TV in the posterosuperior region of the condyles inPanand higher BV/TV and anisotropy in the posteroinferior region inHomo. Furthermore, trabecular number, spacing and thickness differ significantly, mainly separatingGorillafrom the other apes.DiscussionThe trabecular architecture of the distal femur holds a functional signal linked to habitual behaviour; however, there was more similarity across taxa and greater intraspecific variability than expected. Specifically, there was a large degree of overlap in trabecular structure across the sample, andHomowas not as distinct as predicted. Nonetheless, this study offers a comparative sample of trabecular structure in the hominoid distal femur and can contribute to future studies of locomotion in extinct taxa.

Effect of rolling deformation on microstructure and mechanical properties of 2205 duplex stainless steel with micro-nano structure

2020 ◽  
Vol 34 (25) ◽  
pp. 2050269
Author(s):  
Yuqi Mao ◽  
Yuehong Zheng ◽  
Yu Shi ◽  
Min Zhu ◽  
Saitejin ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Stainless Steel ◽  
Duplex Stainless Steel ◽  
Volume Fraction ◽  
Two Phase ◽  
Corrosion Properties ◽  
Nano Structure ◽  
2205 Duplex Stainless Steel ◽  
Microstructure And Mechanical Properties ◽  
Rolling Deformation

In order to further expand the application scope of 2205 duplex stainless steel (DSS), its microstructure and mechanical properties require as much attention as its corrosion properties. In this study, 2205DSSs were prepared by aluminothermic reaction and the microstructures and mechanical behavior of the rolled alloys were analyzed. The micro-nanocrystals composite structure appears in the alloys after rough rolling with deformation of 40% at [Formula: see text]C followed by finishing rolling with deformation of 30%, 50% and 70% at [Formula: see text]C. With the increase of rolling deformation, the two-phase structure is gradually elongated, the average size of the two-phase grains is gradually increased, and some [Formula: see text] phase will change to [Formula: see text] phase, the volume fraction of [Formula: see text] phase is gradually increased, and the distribution of nanocrystals is gradually uniform. Meanwhile, the fracture mode of alloy is gradually changed from ductile fracture to brittle fracture. The strength and hardness of the alloy increase gradually.

Isothermal Transformation, Microstructure and Mechanical Properties of Ausformed Low-Carbon Carbide-Free Bainitic Steel

2018 ◽  
Vol 941 ◽  
pp. 329-333 ◽  
Author(s):  
Jiang Ying Meng ◽  
Lei Jie Zhao ◽  
Fan Huang ◽  
Fu Cheng Zhang ◽  
Li He Qian
Keyword(s):  
Mechanical Properties ◽  
Retained Austenite ◽  
Volume Fraction ◽  
Bainitic Ferrite ◽  
Isothermal Transformation ◽  
Bainitic Transformation ◽  
Isothermal Treatment ◽  
Bainitic Steel ◽  
Low Carbon ◽  
Microstructure And Mechanical Properties

In the present study, the effects of ausforming on the bainitic transformation, microstructure and mechanical properties of a low-carbon rich-silicon carbide-free bainitic steel have been investigated. Results show that prior ausforming shortens both the incubation period and finishing time of bainitic transformation during isothermal treatment at a temperature slightly above the Mspoint. The thicknesses of bainitic ferrite laths are reduced appreciably by ausforming; however, ausforming increases the amount of large blocks of retained austenite/martenisite and decreases the volume fraction of retained austenite. And accordingly, ausforming gives rise to significant increases in both yield and tensile strengths, but causes noticeable decreases in ductility and impact toughness.

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