Differences in tissue-specific and embryonic expression of mouse Ceacam1 and Ceacam2 genes

2001 ◽  
Vol 355 (2) ◽  
pp. 417-423 ◽  
Author(s):  
Eric HAN ◽  
Dillon PHAN ◽  
Piao LO ◽  
Matthew N. POY ◽  
Richard BEHRINGER ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Adhesion Molecule ◽  
Reverse Transcription ◽  
Expression Patterns ◽  
Tissue Expression ◽  
Intercellular Adhesion Molecule ◽  
Mouse Development ◽  
Nucleotide Substitutions ◽  
Reverse Transcription Pcr ◽  
Mouse Tissues

The intercellular adhesion molecule CEACAM1, also known as C-CAM1 (where CAM is cell-adhesion molecule), can function as a tumour suppressor in several carcinomas, including those of the prostate, breast, bladder and colon. This suggests that CEACAM1 may play an important role in the regulation of normal cell growth and differentiation. However, there is no direct evidence to support this putative function of CEACAM1. To elucidate its physiological function by targeted gene deletion, we isolated the Ceacam genes from a mouse 129 Sv/Ev library. Although there is only one Ceacam1 gene in humans and one in rats, two homologous genes (Ceacam1 and Ceacam2) have been identified in the mouse. Our sequence analysis revealed that the genes encoded nine exons and spanned approx. 16-17 kb (Ceacam1) and 25kb (Ceacam2). The genes were highly similar (79.6%). The major differences in the protein-coding regions were located in exons 2, 5 and 6 (76.9%, 87.0% and 78.5% similarity respectively). In addition, introns 2, 5 and 7 were also significantly different, being 29.7%, 59.8% and 64.5% similar respectively. While most of these differences were due to nucleotide substitutions, two insertions of 418 and 5849bp occurred in intron 2 of Ceacam2, and another two insertions of 1384 and 197bp occurred in introns 5 and 7 respectively. To determine whether functional redundancy exists between Ceacam1 and Ceacam2, we examined their expression in 16 mouse tissues by using semi-quantitative reverse transcription-PCR. As in human and rat, in the mouse Ceacam1 mRNA was highly abundant in the liver, small intestine, prostate and spleen. In contrast, Ceacam2 mRNA was only detected in kidney, testis and, to a lesser extent, spleen. Reverse transcription-PCR using testis RNA indicated that Ceacam2 in the testis is an alternatively spliced form containing only exons 1, 2, 5, 6, 8 and 9. In the mouse embryo, Ceacam1 mRNA was detected at day 8.5, disappeared between days 9.5 and 12.5, and re-appeared at day 19. On the other hand, no Ceacam2 mRNA was detected throughout embryonic development. The different tissue expression patterns and regulation during embryonic development suggest that the CEACAM1 and CEACAM2 proteins, although highly similar, may have different functions both during mouse development and in adulthood. The Ceacam1 and Ceacam2 sequences have been deposited in the GenBank®/EMBL/DDBJ/GSDB Nucleotide Sequence Databases with accession numbers AF287911 and AF287912 respectively.

scholarly journals Intercellular Adhesion Molecule-1 and Hair Follicle Regression

2000 ◽  
Vol 48 (4) ◽  
pp. 557-568 ◽  
Author(s):  
Sven Müller-Röver ◽  
Silvia Bulfone-Paus ◽  
Bori Handjiski ◽  
Pia Welker ◽  
John P. Sundberg ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Adhesion Molecule ◽  
Expression Patterns ◽  
Intercellular Adhesion ◽  
Hair Follicles ◽  
Intercellular Adhesion Molecule ◽  
Intercellular Adhesion Molecule 1 ◽  
Outer Root Sheath ◽  
Cycle Dependent ◽  
Connective Tissue Sheath

Although the intercellular adhesion molecule-1 (ICAM-1) is recognized for its pivotal role in inflammation and immune responses, its role in developmental systems, such as the cyclic growth (anagen) and regression (catagen) of the hair follicle, remains to be explored. Here we demonstrate that ICAM-1 expression in murine skin is even more widespread and more developmentally regulated than was previously believed. In addition to endothelial cells, selected epidermal and follicular keratinocyte subpopulations, as well as interfollicular fibroblasts, express ICAM-1. Murine hair follicles express ICAM-1 only late during morphogenesis. Thereafter, morphologically identical follicles markedly differ in their ICAM-1 expression patterns, which become strikingly hair cycle-dependent in both intra- and extrafollicular skin compartments. Minimal ICAM-1 and leukocyte function-associated (LFA-1) protein and mRNA expression is observed during early anagen and maximal expression during late anagen and catagen. Keratinocytes of the distal outer root sheath, fibroblasts of the perifollicular connective tissue sheath, and perifollicular blood vessels exhibit maximal ICAM-1 immunoreactivity during catagen, which corresponds to changes of LFA-1 expression on perifollicular macrophages. Finally, ICAM-1-deficient mice display significant catagen acceleration compared to wild-type controls. Therefore, ICAM-1 upregulation is not limited to pathological situations but is also important for skin and hair follicle remodeling. Collectively, this suggests a new and apparently nonimmunological function for ICAM-1-related signaling in cutaneous biology.

scholarly journals Oestrogen receptor knockout mice: roles for oestrogen receptors alpha and beta in reproductive tissues

Reproduction ◽  
2003 ◽  
pp. 143-149 ◽  
Author(s):  
SC Hewitt ◽  
KS Korach
Keyword(s):  
Oestrogen Receptor ◽  
Expression Patterns ◽  
Tissue Expression ◽  
Genetically Engineered ◽  
Oestrogen Receptors ◽  
Female Reproduction ◽  
Receptor Alpha ◽  
Reproductive Tissues ◽  
Oestrogen Receptor Alpha ◽  
Mouse Tissues

Oestrogen is an essential component of female reproduction, with well-characterized functions in the uterus, ovaries, mammary gland and hypothalamic-pituitary axis. The mechanism of oestrogen action involves mediation of the rate of transcription by nuclear-localized oestrogen receptor molecules. Two oestrogen receptors are present in mouse tissues, oestrogen receptors alpha and beta. Each receptor exhibits differential tissue expression patterns. Mouse models with genetically engineered disruption or 'knockout' of the oestrogen receptors have been developed. Characterization of the resulting defects in reproductive tissues as well as alterations in physiological and genomic responses has given insight into the receptor-mediated effects of oestrogen in reproduction. Oestrogen receptor alpha knockout females are infertile because they are anovulatory, have disruption in LH regulation and have uteri that are insensitive to oestrogen. In contrast, oestrogen receptor beta knockout females are sub-fertile and primarily lack efficient ovulatory function. Mice with deletion of both oestrogen receptors alpha and beta are similar to those lacking oestrogen receptor alpha only, but exhibit a unique ovarian pathology. These observed phenotypes elucidate the relative roles of the oestrogen receptors in reproductive functions of female rodents.

Expression patterns of Nlrp9a, Nlrp9b and Nlrp9c during mouse development

Biologia ◽  
2014 ◽  
Vol 69 (1) ◽  
Author(s):  
Hui Peng ◽  
Wenchang Zhang ◽  
Tianfang Xiao ◽  
Yong Zhang
Keyword(s):  
Preimplantation Embryo ◽  
Expression Patterns ◽  
Critical Role ◽  
Maternal Origin ◽  
Mouse Development ◽  
Cell Stage ◽  
Preimplantation Embryo Development ◽  
Mouse Tissues ◽  
Oocyte Expression ◽  
Oocyte Aging

AbstractThe Nlrp gene family contains 20 members in the mouse. Recent studies have demonstrated that these genes play key roles in reproduction. In this study, we investigated the expression patterns of Nlrp9a, Nlrp9b and Nlrp9c in the mouse. In 2-week-old mouse tissues, Nlrp9a, Nlrp9b and Nlrp9c were all strikingly expressed in the ovary, while the transcripts of Nlrp9b and Nlrp9c were also detected in other tissues. The transcripts of Nlrp9a, Nlrp9b and Nlrp9c were restricted to the oocytes and declined with oocyte aging within the ovary. Furthermore, Nlrp9a, Nlrp9b and Nlrp9c transcripts presented evidence for the exclusive maternal origin, which were presented in oocytes and zygotes, immediately downregulated and not detected after the 2-cell stage during preimplantation development. In addition, Nlrp9a and Nlrp9c transcripts were not detected in other cells except for oocytes. Nevertheless, Nlrp9b expression was detected in oocytes, as well as in D3 ES and F9 ES. These results indicate that Nlrp9a, Nlrp9b and Nlrp9c display specific or preferential oocyte expression patterns and may play critical role in oogenesis and/or preimplantation embryo development in the mouse.

scholarly journals Identification of Salt-Stress-Induced Genes from the RNA-Seq Data ofReaumuria trigynaUsing Differential-Display Reverse Transcription PCR

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Zhen-hua Dang ◽  
Qi Qi ◽  
Hui-rong Zhang ◽  
Hao-yu Li ◽  
Shu-Biao Wu ◽  
...  
Keyword(s):  
Salt Stress ◽  
Differential Display ◽  
Reverse Transcription ◽  
Expression Patterns ◽  
Digital Gene Expression ◽  
Rna Seq ◽  
Sequencing Data ◽  
Reverse Transcription Pcr ◽  
Wide Range ◽  
Differential Display Reverse Transcription

Next generation sequencing (NGS) technologies have been used to generate huge amounts of sequencing data from many organisms. However, the correct choice of candidate genes and prevention of false-positive results computed from digital gene expression (DGE) of RNA-seq data are vital when using these genetic resources. We indirectly identified 18 salt-stress-inducedReaumuria trigynatranscripts from the transcriptome sequencing data using differential-display reverse transcription PCR (DDRT-PCR) combined with local BLAST searches. Highly consistent with the DGE results, the quantitative real-time PCR expression patterns of these transcripts showed strong upregulation by salt stress, suggesting that these genes may play important roles inR. trigyna’s survival under high-salt environments. The method presented here successfully identified responsive genes from the massive amount of RNA-seq data. Thus, we suggest that DDRT-PCR could be employed to mine NGS data in a wide range of applications in transcriptomic studies. In addition, the genes identified in the present study are promising candidates for further elucidation of the salt tolerance mechanisms inR. trigyna.

scholarly journals Cables2 Is a Novel Smad2-Regulatory Factor Essential for Early Embryonic Development in Mice

2019 ◽  
Author(s):  
Tra Thi Huong Dinh ◽  
Hiroyoshi Iseki ◽  
Seiya Mizuno ◽  
Saori Iijima-Mizuno ◽  
Yoko Tanimoto ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Expression Patterns ◽  
Physiological Role ◽  
Primitive Streak ◽  
Early Embryonic Development ◽  
Visceral Endoderm ◽  
Tetraploid Complementation ◽  
Mouse Tissues

ABSTRACTCDK5 and Abl enzyme substrate 2 (Cables2), a member of the Cables family that has a C-terminal cyclin box-like domain, is widely expressed in adult mouse tissues. However, the physiological role of Cables2 in vivo is unknown. We show here that Cables2-deficiency causes post-gastrulation embryonic lethality in mice. The mutant embryos progress to gastrulation, but then arrest, and fail to grow. Analysis of gene expression patterns reveals that formation of the anterior visceral endoderm and the primitive streak is impaired in Cables2-deficient embryos. Tetraploid complementation analyses support the critical requirement of Cables2 in both the epiblast and visceral endoderm for progression of embryogenesis. In addition, we show that Cables2 physically interacts with a key mediator of the canonical Nodal pathway, Smad2, and augments its transcriptional activity. These findings provide novel insights into the essential role of Cables2 in the early embryonic development in mice.

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