scholarly journals Oestrogen receptor knockout mice: roles for oestrogen receptors alpha and beta in reproductive tissues

Reproduction ◽  
2003 ◽  
pp. 143-149 ◽  
Author(s):  
SC Hewitt ◽  
KS Korach
Keyword(s):  
Oestrogen Receptor ◽  
Expression Patterns ◽  
Tissue Expression ◽  
Genetically Engineered ◽  
Oestrogen Receptors ◽  
Female Reproduction ◽  
Receptor Alpha ◽  
Reproductive Tissues ◽  
Oestrogen Receptor Alpha ◽  
Mouse Tissues

Oestrogen is an essential component of female reproduction, with well-characterized functions in the uterus, ovaries, mammary gland and hypothalamic-pituitary axis. The mechanism of oestrogen action involves mediation of the rate of transcription by nuclear-localized oestrogen receptor molecules. Two oestrogen receptors are present in mouse tissues, oestrogen receptors alpha and beta. Each receptor exhibits differential tissue expression patterns. Mouse models with genetically engineered disruption or 'knockout' of the oestrogen receptors have been developed. Characterization of the resulting defects in reproductive tissues as well as alterations in physiological and genomic responses has given insight into the receptor-mediated effects of oestrogen in reproduction. Oestrogen receptor alpha knockout females are infertile because they are anovulatory, have disruption in LH regulation and have uteri that are insensitive to oestrogen. In contrast, oestrogen receptor beta knockout females are sub-fertile and primarily lack efficient ovulatory function. Mice with deletion of both oestrogen receptors alpha and beta are similar to those lacking oestrogen receptor alpha only, but exhibit a unique ovarian pathology. These observed phenotypes elucidate the relative roles of the oestrogen receptors in reproductive functions of female rodents.

scholarly journals Expression of oxytocin, oestrogen and progesterone receptors in uterine biopsy samples throughout the oestrous cycle and early pregnancy in cows

Reproduction ◽  
2001 ◽  
pp. 965-979 ◽  
Author(s):  
RS Robinson ◽  
GE Mann ◽  
GE Lamming ◽  
DC Wathes
Keyword(s):  
Progesterone Receptor ◽  
Early Pregnancy ◽  
Oestrogen Receptor ◽  
Expression Patterns ◽  
Oestrous Cycle ◽  
Receptor Expression ◽  
Luminal Epithelium ◽  
Receptor Alpha ◽  
Oestrogen Receptor Alpha ◽  
Oxytocin Receptors

This study examined the expression patterns of oxytocin and steroid receptors in the bovine endometrium during the oestrous cycle and early pregnancy to elucidate their respective roles in the regulation of luteolysis and the maternal recognition of pregnancy. In Expt 1, uterine biopsies were collected from four cows throughout three oestrous cycles each, to provide daily samples. In Expt 2, uterine tissue was collected on days 12, 14, 16 and 18 of the oestrous cycle (n = 20) or early pregnancy (n = 16). Oxytocin receptor, oestrogen receptor alpha and progesterone receptor mRNAs were localized by in situ hybridization, and localization of oestrogen receptor and progesterone receptor was confirmed by immunocytochemistry. All three receptors showed time- and cell-specific expression patterns. Oestrogen receptor alpha increased in all regions at oestrus but high concentrations were also found in the luminal epithelium during the mid-luteal phase and in the deep glands throughout the oestrous cycle. Progesterone receptor expression was higher in the stroma than it was in the types of epithelial cell, and increased expression was observed at oestrus and during the early luteal phase. The cyclical upregulation of oxytocin receptors in the luminal epithelium on about day 16 was not related to preceding changes in the endometrial expression of either oestradiol alpha or progesterone receptors. During early pregnancy, oxytocin receptor expression was suppressed. Oestrogen receptor a concentrations increased in the non-pregnant cows and decreased in the pregnant cows between days 16 and 18, but these changes followed rather than preceded the upregulation of oxytocin receptors in the non-pregnant cows. It is concluded that the initial upregulation of oxytocin receptors in the luminal epithelium, which triggers luteolysis, is not associated directly with changes in expression of oestrogen receptor alpha.

A possible divergent role for the oestrogen receptor alpha and beta subtypes in clinical breast cancer

2000 ◽  
Vol 89 (2) ◽  
pp. 209-212 ◽  
Author(s):  
Janice M. Knowlden ◽  
Julia M.W. Gee ◽  
John F.R. Robertson ◽  
Ian O. Ellis ◽  
Robert I. Nicholson
Keyword(s):  
Breast Cancer ◽  
Oestrogen Receptor ◽  
Receptor Alpha ◽  
Oestrogen Receptor Alpha ◽  
Clinical Breast

Oestrogen receptor alpha expression in neovaginal tissue of women following modified Abbé-McIndoe technique and in premenopausal women

2015 ◽  
Vol 31 (4) ◽  
pp. 327-331
Author(s):  
Marcio Masashi Kajikawa ◽  
Zsuzsanna Ilona Katalin Jármy-Di Bella ◽  
Juliane Dornelas ◽  
Luciana Campanatti Crema ◽  
Cláudia Cristina Takano ◽  
...  
Keyword(s):  
Oestrogen Receptor ◽  
Premenopausal Women ◽  
Receptor Alpha ◽  
Oestrogen Receptor Alpha

scholarly journals Oestrogen's masculine side: mediation of mating in male mice

Reproduction ◽  
2002 ◽  
pp. 331-338 ◽  
Author(s):  
EM Scordalakes ◽  
DB Imwalle ◽  
EF Rissman
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Dopamine Agonist ◽  
Sexual Behaviour ◽  
Oestrogen Receptor ◽  
Neuronal Nitric Oxide Synthase ◽  
Receptor Alpha ◽  
Male Sexual Behaviour ◽  
Oestrogen Receptor Alpha ◽  
Oxide Synthase

This review focuses on the role of oestrogen in male sexual behaviour using oestrogen receptor alpha and beta knockout (ERalphaKO and ERbetaKO) mouse models. ERbetaKO mice are capable of mating and producing offspring, whereas ERalphaKO mice are unable to do either. When ERalphaKO males are treated with testosterone or dihydrotestosterone (DHT), < 50% display mounting behaviour, few intromit and none ejaculate. However, concurrent treatment with testosterone and a dopamine agonist instates masculine sexual behaviour in both male and female ERalphaKO mice. Dopamine content in the preoptic area and associated regions is not affected by oestrogen receptor alpha gene disruption. However, expression of neuronal nitric oxide synthase immunoreactivity is severely reduced in ERalphaKO males compared with wild-type males. These findings, together with studies conducted in aromatase knockout mice, are at odds with the dogma that oestrogen is required during development for expression of male sexual behaviour in adults. However, they do support a role for oestrogens, mediated by oestrogen receptor alpha, in regulation and production of neuronal nitric oxide synthase, which in turn may control dopamine agonist release. As has been shown in male rats, in mice dopamine agonist release is likely to be an essential component of the neural pathway that mediates male sexual behaviour.

scholarly journals Hypothalamic oestrogen receptor alpha establishes a sexually dimorphic regulatory node of energy expenditure

2020 ◽  
Vol 2 (4) ◽  
pp. 351-363 ◽  
Author(s):  
J. Edward van Veen ◽  
Laura G. Kammel ◽  
Patricia C. Bunda ◽  
Michael Shum ◽  
Michelle S. Reid ◽  
...  
Keyword(s):  
Energy Expenditure ◽  
Oestrogen Receptor ◽  
Sexually Dimorphic ◽  
Receptor Alpha ◽  
Oestrogen Receptor Alpha
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