scholarly journals Purification and characterization of oil-bodies (oleosomes) and oil-body boundary proteins (oleosins) from the developing cotyledons of sunflower (Helianthus annuus L.)

1996 ◽  
Vol 314 (1) ◽  
pp. 333-337 ◽  
Author(s):  
Mark MILLICHIP ◽  
Arthur S. TATHAM ◽  
Frances JACKSON ◽  
Gareth GRIFFITHS ◽  
Peter R. SHEWRY ◽  
...  
Keyword(s):  
Helianthus Annuus ◽  
Storage Proteins ◽  
Phospholipid Content ◽  
Major Protein ◽  
Oil Body ◽  
Oil Bodies ◽  
Helianthus Annuus L ◽  
Novel Structure ◽  
Body Boundary ◽  
Helical Proteins

Oil-bodies, from the immature cotyledons of sunflower (Helianthus annuus L.), were difficult to purify to homogeneity using conventional techniques. The major protein contaminants were albumin and globulin storage proteins. A protocol has been developed, therefore, based upon the stringent washing of the oil-body fraction in 9 M urea, which effectively removed almost all the contaminating protein as judged by SDS/PAGE. The urea-washed oil-bodies were enriched in two major proteins of Mr 19000 and 20000. These proteins were oleosins as demonstrated by their amino acid compositions and the sequence analysis of peptides produced by CNBr cleavage. Far-UV CD spectra of the oleosins in trifluoroethanol, trifluoroethanol/water mixtures and as mixed micelles in SDS, were typical of α-helical proteins with α-helical contents of some 55%. The phospholipid content of the urea-washed preparations was less than 0.1% of that required to form a half-unit membrane surrounding the oil-body. The oil-body surface therefore appears to be an unusual and novel structure, covered largely by an oleosin protein coat or pellicle rather than a conventional fluid membrane, half-unit or otherwise.

scholarly journals Synthesis of the major oil-body membrane protein in developing rapeseed (Brassica napus) embryos. Integration with storage-lipid and storage-protein synthesis and implications for the mechanism of oil-body formation

1989 ◽  
Vol 258 (1) ◽  
pp. 285-293 ◽  
Author(s):  
D J Murphy ◽  
I Cummins ◽  
A S Kang
Keyword(s):  
Brassica Napus ◽  
Seed Development ◽  
Storage Proteins ◽  
Gradient Centrifugation ◽  
Major Protein ◽  
Oil Body ◽  
Oil Bodies ◽  
Electron Microscopic ◽  
Sucrose Density Gradient Centrifugation ◽  
Body Protein

The synthesis of the major protein and lipid storage reserves during embryogenesis in oilseed rape (Brassica napus L., cv. Mikado) has been examined by biochemical, immunological and immunocytochemical techniques. The mature seeds contained about 45% (w/w) storage oil and 25% (w/w) protein. There were three major seed protein components, i.e. about 40-50% total protein was cruciferin, 20% was napin and 20% was a 18 kDa hydrophobic polypeptide associated with the proteinaceous membrane surrounding the storage oil bodies. Embryogenesis was divided into four overlapping stages with regard to the synthesis of these storage components: (1) for the first 3 weeks after flowering, little, if any, synthesis of storage components was observed; (2) storage-oil synthesis began at about week 3, and maximal rates were from weeks 4 to 7; (3) synthesis of the soluble storage proteins cruciferin and napin started at week 6 and rates were maximal between weeks 8 and 11; (4) the final stage was the synthesis of the 19 kDa oil-body polypeptide, which started at weeks 8-10 and was at a maximal rate between weeks 10 and 12. The synthesis of the 19 kDa oil-body protein therefore occurred independently of the synthesis of the soluble seed storage proteins. This former synthesis did not occur until shortly before the insertion of the 19 kDa polypeptide into the oil-body membrane. No evidence was found, either from sucrose-density-gradient-centrifugation experiments or from immunogold-labelling studies, for its prior accumulation in the endoplasmic reticulum. Conventional and immunogold-electron-microscopic studies showed that oil bodies were synthesized in the early to middle stages of seed development without a strongly electron-dense membrane. Such a membrane was only found at later stages of seed development, concomitantly with the synthesis of the 19 kDa protein. It is proposed that, in rapeseed embryos, oil bodies are initially formed with no proteinaceous membrane. Such a membrane is formed later in development after insertion by ribosomes of the hydrophobic 19 kDa polypeptide directly into the oil bodies.

scholarly journals Secondary structure of oleosins in oil bodies isolated from seeds of safflower (Carthamus tinctorius L.) and sunflower (Helianthus annuus L.)

1998 ◽  
Vol 334 (2) ◽  
pp. 469-477 ◽  
Author(s):  
Dominic J. LACEY ◽  
Nikolaus WELLNER ◽  
Frederic BEAUDOIN ◽  
Johnathan A. NAPIER ◽  
Peter R. SHEWRY
Keyword(s):  
Fourier Transform ◽  
Helianthus Annuus ◽  
Carthamus Tinctorius ◽  
Oil Body ◽  
Oil Bodies ◽  
Hydrophobic Domain ◽  
Helianthus Annuus L ◽  
Molecular Masses ◽  
Mature Seeds ◽  
Protected Peptides

Oil bodies were isolated from mature seeds of sunflower (Helianthus annuus L.) and safflower (Carthamus tinctorius L.). Oil body preparations containing only oleosin proteins could be obtained from safflower seeds by salt-washing followed by centrifugation on discontinuous sucrose density gradients. However, it was necessary to treat sunflower oil bodies with urea to obtain preparations of similar purity. Incubation of the oil bodies with proteinases gave two fragments with molecular masses of 6 and 8 kDa which were protected from digestion. These fragments represented the hydrophobic domain of the oleosins, as determined by N-terminal sequencing. Intact and proteinase-treated oil bodies of both species were analysed by Fourier-transform infrared spectroscopy, as dry films and in aqueous medium, the spectra being compared with those obtained for pure oil samples in order to identify the bands resulting from the oleosin proteins and protected peptides. This investigation showed that the hydrophobic domain of the oleosins in intact oil bodies is predominantly α-helical in structure and that the conformation was not greatly affected by washing the oil bodies with urea during preparation.

scholarly journals Oil-bodies from sunflower (Helianthus annuus L.) seeds

1996 ◽  
Vol 317 (3) ◽  
pp. 955-956 ◽  
Author(s):  
Frédéric BEISSON ◽  
Nathalie FERTE ◽  
Georges NOAT
Keyword(s):  
Helianthus Annuus ◽  
Oil Bodies ◽  
Helianthus Annuus L

Characterization of Helianthus annuus L. storage proteins

1989 ◽  
Vol 37 (4) ◽  
pp. 852-855 ◽  
Author(s):  
M. Durante ◽  
R. Bernardi ◽  
M. C. Lupi ◽  
P. Sabelli
Keyword(s):  
Helianthus Annuus ◽  
Storage Proteins ◽  
Helianthus Annuus L

Impact of extraneous proteins on the gastrointestinal fate of sunflower seed (Helianthus annuus) oil bodies: a simulated gastrointestinal tract study

2015 ◽  
Vol 6 (1) ◽  
pp. 124-133 ◽  
Author(s):  
Sakunkhun Makkhun ◽  
Amit Khosla ◽  
Tim Foster ◽  
David Julian McClements ◽  
Myriam M. L. Grundy ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Helianthus Annuus ◽  
Sunflower Seed ◽  
Oil Body ◽  
Oil Bodies ◽  
Gastrointestinal Conditions

The fate of oil body and protein enriched oil body during digestion under simulated gastrointestinal conditions was studied.

THE PROBLEM OF SAFE SUNFLOWER (Helianthus annuus L.) USE FOR FOOD AND FODDER PURPOSES (review)

2018 ◽  
Vol 53 (3) ◽  
pp. 485-498
Author(s):  
G.P. Kononenko ◽  
◽  
M.I. Ustyuzhanina ◽  
A.A. Burkin ◽  
◽  
...  
Keyword(s):  
Helianthus Annuus ◽  
Helianthus Annuus L

Sunflowers (Helianthus annuus L.) on Top of a Constructed Wetland as an Engineered Ecosystem to Clean Sewage Onsite

2014 ◽  
Vol 2014 (2) ◽  
pp. 83-91
Author(s):  
Alfredo Garcia-Perez ◽  
◽  
Mark Harrison ◽  
Bill Grant ◽  
◽  
...  
Keyword(s):  
Helianthus Annuus ◽  
Constructed Wetland ◽  
Helianthus Annuus L
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