scholarly journals Regulation of fos–lacZ fusion gene expression in primary mouse epidermal keratinocytes isolated from transgenic mice

1995 ◽  
Vol 306 (3) ◽  
pp. 879-879
Keyword(s):  
Gene Expression ◽  
Transgenic Mice ◽  
Fusion Gene ◽  
Lacz Fusion ◽  
Epidermal Keratinocytes ◽  
Primary Mouse ◽  
Mouse Epidermal Keratinocytes

scholarly journals Regulation of fos-lacZ fusion gene expression in primary mouse epidermal keratinocytes isolated from transgenic mice

1994 ◽  
Vol 300 (1) ◽  
pp. 263-270 ◽  
Author(s):  
W B Bollag ◽  
Y Xiong ◽  
J Ducote ◽  
C S Harmon
Keyword(s):  
Transgenic Mice ◽  
Gene Product ◽  
Fusion Gene ◽  
Lacz Fusion ◽  
Northern Analysis ◽  
Epidermal Keratinocytes ◽  
Lacz Expression ◽  
High Calcium ◽  
Primary Mouse ◽  
Mouse Epidermal Keratinocytes

The expression of a fos-lacZ fusion gene was studied in primary mouse epidermal keratinocytes obtained from transgenic mice. This gene construct contains the entire upstream regulatory sequence of c-fos, and expression of the endogenous and fusion gene was shown by Northern analysis to correlate upon induction with the phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA). Using a chromogenic substrate of beta-galactosidase, we also demonstrated that expression of the fusion gene product, like that of Fos, was localized to the cell nucleus. In addition, we showed that epidermal keratinocytes responded to dialysed fetal bovine serum (FBS), TPA and high-calcium medium with enhanced Fos-lacZ expression and an inhibition of proliferation. The time course of induction of Fos-lacZ expression was similar for dialysed FBS and TPA, with a peak approximately 2 h after exposure. Exposure for approximately 24 h to an elevated extracellular calcium concentration was required to elicit an increase in Fos-lacZ expression. The lack of an immediate effect of raising medium calcium levels on Fos-lacZ expression contrasted with the rapidity of its effect on DNA synthesis, which was significantly inhibited within 6-8 h. In addition, we found that the protein kinase C inhibitor Ro 31-7549 blocked Fos-lacZ expression induced by TPA but had little or no effect on that elicited by high calcium levels. Thus, although our results indicate that the fos gene product may be involved in mediating epidermal keratinocyte growth arrest in response to differentiative agents such as FBS, TPA and high medium calcium levels, the exact role of this gene product remains unclear.

Analysis of genomic regions involved in regulation of the rabbit surfactant protein A gene in transgenic mice

1999 ◽  
Vol 277 (2) ◽  
pp. L349-L361 ◽  
Author(s):  
Joseph L. Alcorn ◽  
Robert E. Hammer ◽  
Katherine R. Graves ◽  
Margaret E. Smith ◽  
Shanna D. Maika ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transgenic Mice ◽  
Transgene Expression ◽  
Fusion Gene ◽  
Surfactant Protein ◽  
Type Ii Cells ◽  
Type Ii ◽  
Regulation Of Expression ◽  
Camp Induction ◽  
Genomic Regions

The gene encoding surfactant protein (SP) A, a developmentally regulated pulmonary surfactant-associated protein, is expressed in a lung-specific manner, primarily in pulmonary type II cells. SP-A gene transcription in the rabbit fetal lung is increased by cAMP. To delineate the genomic regions involved in regulation of SP-A gene expression, lines of transgenic mice carrying fusion genes composed of various amounts of 5′-flanking DNA from the rabbit SP-A gene linked to the human growth hormone structural gene as a reporter were established. We found that as little as 378 bp of 5′-flanking DNA was sufficient to direct appropriate lung cell-selective and developmental regulation of transgene expression. The same region was also sufficient to mediate cAMP induction of transgene expression. Mutagenesis or deletion of either of two DNA elements, proximal binding element and a cAMP response element-like sequence, previously found to be crucial for cAMP induction of SP-A promoter activity in transfected type II cells, did not affect lung-selective or temporal regulation of expression of the transgene; however, overall levels of fusion gene expression were reduced compared with those of wild-type transgenes.

scholarly journals 1,25-Dihydroxyvitamin D3Induces Phospholipase D-1 Expression in Primary Mouse Epidermal Keratinocytes

1999 ◽  
Vol 274 (8) ◽  
pp. 4663-4670 ◽  
Author(s):  
Richard D. Griner ◽  
Feng Qin ◽  
EunMi Jung ◽  
Christopher K. Sue-Ling ◽  
Kimberly B. Crawford ◽  
...  
Keyword(s):  
Phospholipase D ◽  
Epidermal Keratinocytes ◽  
Primary Mouse ◽  
Mouse Epidermal Keratinocytes

scholarly journals Metallothionein-vasopressin fusion gene expression in transgenic mice

1989 ◽  
Vol 264 (31) ◽  
pp. 18844-18852
Author(s):  
J F Habener ◽  
B J Cwikel ◽  
H Hermann ◽  
R E Hammer ◽  
R D Palmiter ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transgenic Mice ◽  
Fusion Gene

Tissue specific expression of an α-skeletal actin-lacZ fusion gene during development in transgenic mice

1994 ◽  
Vol 3 (1) ◽  
pp. 59-66 ◽  
Author(s):  
Emmanuel A. Asante ◽  
Jacqueline M. Boswell ◽  
David W. Burt ◽  
Grahame Bulfield
Keyword(s):  
Transgenic Mice ◽  
Fusion Gene ◽  
Lacz Fusion ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression
Sign in / Sign up

Export Citation Format

Share Document