scholarly journals Modulation of phospholipase D-mediated phosphatidylglycerol formation by differentiating agents in primary mouse epidermal keratinocytes

2003 ◽  
Author(s):  
X Zheng
Keyword(s):  
Phospholipase D ◽  
Epidermal Keratinocytes ◽  
Primary Mouse ◽  
Differentiating Agents ◽  
Mouse Epidermal Keratinocytes

scholarly journals 1,25-Dihydroxyvitamin D3Induces Phospholipase D-1 Expression in Primary Mouse Epidermal Keratinocytes

1999 ◽  
Vol 274 (8) ◽  
pp. 4663-4670 ◽  
Author(s):  
Richard D. Griner ◽  
Feng Qin ◽  
EunMi Jung ◽  
Christopher K. Sue-Ling ◽  
Kimberly B. Crawford ◽  
...  
Keyword(s):  
Phospholipase D ◽  
Epidermal Keratinocytes ◽  
Primary Mouse ◽  
Mouse Epidermal Keratinocytes

scholarly journals Regulation of fos-lacZ fusion gene expression in primary mouse epidermal keratinocytes isolated from transgenic mice

1994 ◽  
Vol 300 (1) ◽  
pp. 263-270 ◽  
Author(s):  
W B Bollag ◽  
Y Xiong ◽  
J Ducote ◽  
C S Harmon
Keyword(s):  
Transgenic Mice ◽  
Gene Product ◽  
Fusion Gene ◽  
Lacz Fusion ◽  
Northern Analysis ◽  
Epidermal Keratinocytes ◽  
Lacz Expression ◽  
High Calcium ◽  
Primary Mouse ◽  
Mouse Epidermal Keratinocytes

The expression of a fos-lacZ fusion gene was studied in primary mouse epidermal keratinocytes obtained from transgenic mice. This gene construct contains the entire upstream regulatory sequence of c-fos, and expression of the endogenous and fusion gene was shown by Northern analysis to correlate upon induction with the phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA). Using a chromogenic substrate of beta-galactosidase, we also demonstrated that expression of the fusion gene product, like that of Fos, was localized to the cell nucleus. In addition, we showed that epidermal keratinocytes responded to dialysed fetal bovine serum (FBS), TPA and high-calcium medium with enhanced Fos-lacZ expression and an inhibition of proliferation. The time course of induction of Fos-lacZ expression was similar for dialysed FBS and TPA, with a peak approximately 2 h after exposure. Exposure for approximately 24 h to an elevated extracellular calcium concentration was required to elicit an increase in Fos-lacZ expression. The lack of an immediate effect of raising medium calcium levels on Fos-lacZ expression contrasted with the rapidity of its effect on DNA synthesis, which was significantly inhibited within 6-8 h. In addition, we found that the protein kinase C inhibitor Ro 31-7549 blocked Fos-lacZ expression induced by TPA but had little or no effect on that elicited by high calcium levels. Thus, although our results indicate that the fos gene product may be involved in mediating epidermal keratinocyte growth arrest in response to differentiative agents such as FBS, TPA and high medium calcium levels, the exact role of this gene product remains unclear.

scholarly journals 8-Cl-Adenosine Induces Growth Arrest without Differentiation of Primary Mouse Epidermal Keratinocytes

2001 ◽  
Vol 117 (6) ◽  
pp. 1588-1593 ◽  
Author(s):  
Daniel T. Dransfield ◽  
Richard D. Griner ◽  
Sagarika Ray ◽  
Meral Keskintepe ◽  
Wendy B. Bollag
Keyword(s):  
Growth Arrest ◽  
Epidermal Keratinocytes ◽  
Primary Mouse ◽  
Mouse Epidermal Keratinocytes
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