scholarly journals Heterogeneous modulation of acute-phase-reactant mRNA levels by interleukin-1β and interleukin-6 in the human hepatoma cell line PLC/PRF/5

1992 ◽  
Vol 281 (3) ◽  
pp. 879-879
Keyword(s):  
Cell Line ◽  
Acute Phase ◽  
Interleukin 6 ◽  
Hepatoma Cell ◽  
Acute Phase Reactant ◽  
Hepatoma Cell Line ◽  
Mrna Levels ◽  
Human Hepatoma ◽  
Human Hepatoma Cell ◽  
Phase Reactant

scholarly journals Hypoxia, a Novel Inducer of Acute Phase Gene Expression in a Human Hepatoma Cell Line

1995 ◽  
Vol 270 (46) ◽  
pp. 27865-27870 ◽  
Author(s):  
Roland H. Wenger ◽  
Andreas Rolfs ◽  
Hugo H. Marti ◽  
Christian Bauer ◽  
Max Gassmann
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Acute Phase ◽  
Hepatoma Cell ◽  
Hepatoma Cell Line ◽  
Human Hepatoma ◽  
Human Hepatoma Cell ◽  
Human Hepatoma Cell Line

scholarly journals Heterogeneous modulation of acute-phase-reactant mRNA levels by interleukin-1 β and interleukin-6 in the human hepatoma cell line PLC/PRF/5

1991 ◽  
Vol 277 (2) ◽  
pp. 477-482 ◽  
Author(s):  
D M Steel ◽  
A S Whitehead
Keyword(s):  
Cell Line ◽  
Acute Phase ◽  
Acute Phase Response ◽  
Hepatoma Cell ◽  
Phase Response ◽  
Serum Amyloid ◽  
Hepatoma Cell Line ◽  
Human Hepatoma ◽  
Human Hepatoma Cell ◽  
Human Hepatoma Cell Line

The acute-phase response to tissue injury and inflammation is accompanied by a dramatic increase in the hepatic synthesis of plasma proteins known as acute-phase reactants (APRs). This response is mediated by cytokines produced in part by activated macrophages at the site of inflammation; glucocorticoids have also been implicated as playing a regulatory role. The effects of the cytokines interleukin (IL)-1 beta and -6, alone or in combination, and in the absence or presence of the synthetic glucocorticoid dexamethasone, on the levels of APR mRNAs in the human hepatoma cell line PLC/PRF/5 were analysed. The accumulation of APR mRNAs [the complement components C3, factor B and Cl inhibitor; the major APRs C-reactive protein (CRP) and serum amyloid A protein and the CRP analogue serum amyloid P protein] was determined in dose-response and time-course studies. The APRs differed from each other in their responses to IL-1 beta alone, IL-6 alone, and IL-1 beta plus IL-6. Dexamethasone enhanced the cytokine-driven induction of a subset of APR mRNAs. These studies detail the heterogeneity of the ‘in vitro’ acute-phase response to defined mediators.

scholarly journals Regulation of human GH receptor gene transcription by 20 and 22 kDa GH in a human hepatoma cell line

2000 ◽  
Vol 165 (2) ◽  
pp. 313-320 ◽  
Author(s):  
JM Nuoffer ◽  
C Fluck ◽  
J Deladoey ◽  
A Eble ◽  
MT Dattani ◽  
...  
Keyword(s):  
Cell Line ◽  
Gene Transcription ◽  
Hepatoma Cell ◽  
Receptor Gene ◽  
Hepatoma Cell Line ◽  
Mrna Levels ◽  
Human Hepatoma ◽  
Human Hepatoma Cell ◽  
Human Hepatoma Cell Line ◽  
Gh Receptor

The human GH gene is 1.7 kilobase pairs (kb) in length and is composed of five exons and four introns. This gene is expressed in the pituitary gland and encodes a 22 kDa protein. In addition to this predominant (75%) form, 5-10% of pituitary GH is present as a 20 kDa protein that has an amino acid (aa) sequence identical to the 22 kDa form except for a 15 aa internal deletion of residues 32-46 as a result of an alternative splicing event. Because it has been reported that non-22-kDa GH isoforms might be partly responsible for short stature and growth retardation in children, the aim of this study was to compare the impact of both 22 kDa and 20 kDa GH on GH receptor gene (GH receptor/GH binding protein (GHR/GHBP)) expression. Various concentrations of 20 kDa and 22 kDa GH (0, 2, 5, 12.5, 25, 50 and 150 ng/ml) were added to human hepatoma (HuH7) cells cultured in serum-free hormonally defined medium for 0, 1 and 2 h. Thereafter GHR/GHBP mRNA expression was measured by quantitative PCR. Addition of either 20 kDa or 22 kDa GH, at low or normal physiological concentrations (0, 2, 5, 12.5, 25 or 50 ng/ml) induced a dose-dependent increase in GHR/GHBP expression. However, a supraphysiological concentration of 20 kDa GH (150 ng/ml) resulted in a significantly lower (P<0.05) downregulation of GHR/GHBP gene transcription compared with the downregulation achieved by this concentration of 22 kDa GH. This difference might be explained by a decreased ability to form a 1 : 1 complex with GHR and/or GHBP, which normally occurs at high concentrations of GH. Nuclear run-on experiments and GHBP determinations confirmed the changes in GHR/GHBP mRNA levels. In conclusion, we report that both 20 kDa and 22 kDa GH, in low and normal physiological concentrations, have the same effect on regulation of GHR/GHBP gene transcription in a human hepatoma cell line. At a supraphysiological concentration of 150 ng/ml, however, 20 kDa GH has a less self-inhibitory effect than the 22 kDa form.

Insulin modulation of acute-phase protein production in a human hepatoma cell line

Cytokine ◽  
1991 ◽  
Vol 3 (6) ◽  
pp. 619-626 ◽  
Author(s):  
Douglas Thompson ◽  
Stephen P. Harrison ◽  
Stuart W. Evans ◽  
John T. Whicher
Keyword(s):  
Cell Line ◽  
Acute Phase ◽  
Protein Production ◽  
Acute Phase Protein ◽  
Hepatoma Cell ◽  
Hepatoma Cell Line ◽  
Human Hepatoma ◽  
Human Hepatoma Cell ◽  
Human Hepatoma Cell Line ◽  
Phase Protein
Sign in / Sign up

Export Citation Format

Share Document