Inhibition of the acute-phase response in a human hepatoma cell line

1993 ◽  
Vol 38 (S2) ◽  
pp. C66-C68 ◽  
Author(s):  
J. G. Raynes ◽  
S. Bevan
Keyword(s):  
Cell Line ◽  
Acute Phase ◽  
Acute Phase Response ◽  
Hepatoma Cell ◽  
Phase Response ◽  
Hepatoma Cell Line ◽  
Human Hepatoma ◽  
Human Hepatoma Cell ◽  
Human Hepatoma Cell Line

scholarly journals Heterogeneous modulation of acute-phase-reactant mRNA levels by interleukin-1 β and interleukin-6 in the human hepatoma cell line PLC/PRF/5

1991 ◽  
Vol 277 (2) ◽  
pp. 477-482 ◽  
Author(s):  
D M Steel ◽  
A S Whitehead
Keyword(s):  
Cell Line ◽  
Acute Phase ◽  
Acute Phase Response ◽  
Hepatoma Cell ◽  
Phase Response ◽  
Serum Amyloid ◽  
Hepatoma Cell Line ◽  
Human Hepatoma ◽  
Human Hepatoma Cell ◽  
Human Hepatoma Cell Line

The acute-phase response to tissue injury and inflammation is accompanied by a dramatic increase in the hepatic synthesis of plasma proteins known as acute-phase reactants (APRs). This response is mediated by cytokines produced in part by activated macrophages at the site of inflammation; glucocorticoids have also been implicated as playing a regulatory role. The effects of the cytokines interleukin (IL)-1 beta and -6, alone or in combination, and in the absence or presence of the synthetic glucocorticoid dexamethasone, on the levels of APR mRNAs in the human hepatoma cell line PLC/PRF/5 were analysed. The accumulation of APR mRNAs [the complement components C3, factor B and Cl inhibitor; the major APRs C-reactive protein (CRP) and serum amyloid A protein and the CRP analogue serum amyloid P protein] was determined in dose-response and time-course studies. The APRs differed from each other in their responses to IL-1 beta alone, IL-6 alone, and IL-1 beta plus IL-6. Dexamethasone enhanced the cytokine-driven induction of a subset of APR mRNAs. These studies detail the heterogeneity of the ‘in vitro’ acute-phase response to defined mediators.

scholarly journals Monokines regulate glycosylation of acute-phase proteins.

1987 ◽  
Vol 166 (1) ◽  
pp. 253-258 ◽  
Author(s):  
A Mackiewicz ◽  
M K Ganapathi ◽  
D Schultz ◽  
I Kushner
Keyword(s):  
Cell Line ◽  
Acute Phase ◽  
Acute Phase Response ◽  
Acute Phase Proteins ◽  
Hepatoma Cell ◽  
Phase Response ◽  
Hepatoma Cell Line ◽  
Human Hepatoma Cell ◽  
Inflammatory Stimuli ◽  
Necrosis Factor

The acute-phase response to inflammatory stimuli, characterized by increased synthesis of acute-phase proteins (APP), is often accompanied by changes in the glycosylation patterns of some of these proteins. While expression of APP genes in hepatocytes is regulated by monokines, mechanisms governing changes in glycosylation are not known. Exposure of human hepatoma cell line Hep 3B to conditioned medium from LPS-activated human monocytes and to medium from the keratocarcinoma cell line COLO-16 led to increased synthesis of alpha 1 proteinase-inhibitor and ceruloplasmin and to alterations of their glycosylation patterns similar to those seen in human serum in various inflammatory states. IL-1, tumor necrosis factor, and hepatocyte stimulating factor I increased synthesis of ceruloplasmin without alterations in the pattern of its glycosylation. These findings demonstrate that altered glycosylation seen in plasma in some inflammatory states can be explained by the effects of monokines on glycosylation in hepatocytes and that gene expression and glycosylation of some APP during the acute-phase response may be regulated by different mechanisms.

scholarly journals Hypoxia, a Novel Inducer of Acute Phase Gene Expression in a Human Hepatoma Cell Line

1995 ◽  
Vol 270 (46) ◽  
pp. 27865-27870 ◽  
Author(s):  
Roland H. Wenger ◽  
Andreas Rolfs ◽  
Hugo H. Marti ◽  
Christian Bauer ◽  
Max Gassmann
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Acute Phase ◽  
Hepatoma Cell ◽  
Hepatoma Cell Line ◽  
Human Hepatoma ◽  
Human Hepatoma Cell ◽  
Human Hepatoma Cell Line

Insulin modulation of acute-phase protein production in a human hepatoma cell line

Cytokine ◽  
1991 ◽  
Vol 3 (6) ◽  
pp. 619-626 ◽  
Author(s):  
Douglas Thompson ◽  
Stephen P. Harrison ◽  
Stuart W. Evans ◽  
John T. Whicher
Keyword(s):  
Cell Line ◽  
Acute Phase ◽  
Protein Production ◽  
Acute Phase Protein ◽  
Hepatoma Cell ◽  
Hepatoma Cell Line ◽  
Human Hepatoma ◽  
Human Hepatoma Cell ◽  
Human Hepatoma Cell Line ◽  
Phase Protein
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