scholarly journals Effects of variation in the structure of spermine on the association with DNA and the induction of DNA conformational changes

1990 ◽  
Vol 269 (2) ◽  
pp. 329-334 ◽  
Author(s):  
H S Basu ◽  
H C A Schwietert ◽  
B G Feuerstein ◽  
L J Marton
Keyword(s):  
Melting Temperature ◽  
Conformational Changes ◽  
Carbon Chain ◽  
Calf Thymus Dna ◽  
Carbon Chains ◽  
Calf Thymus ◽  
Central Carbon ◽  
Induced Aggregation ◽  
Dna Complex ◽  
Spermine Analogues

The effects of spermine and spermine analogues on the B-Z transition of poly(dG-me5dC) and on the aggregation and ‘melting’ temperature of calf thymus DNA were studied by spectroscopic methods. The association constants of these polyamines with double- and single-stranded calf thymus DNA were calculated from their effects on the melting temperature. The effect of these compounds on the release of ethidium bromide (EB) from an EB-DNA complex were measured by a spectrofluorimetric method. This efficiency of the polyamine-induced B-Z transition strongly depended on the length of the central carbon chains of the compounds and on the functional groups attached to the carbon chains. Both the terminal primary amino groups and the length of the central carbon chain affected the aggregation of DNA. The affinity of the analogues for DNA increased as the number of n-butyl groups increased, but decreased with either an increase or a decrease in the length of the central carbon chain. The effect of spermine and spermine analogues on the release of EB from an EB-DNA complex did not always correlate with the affinities of analogues for calf thymus DNA. In particular, tetra-amines with more than one n-butyl group bound better to DNA than did spermine, but released bound EB and induced aggregation of DNA less well than did spermine. We postulate that either a bend and/or other localized conformational changes of DNA are responsible for the spermine-induced aggregation of DNA and the release of EB from the EB-DNA complex.

scholarly journals The interaction of spermine and pentamines with DNA

1987 ◽  
Vol 244 (1) ◽  
pp. 243-246 ◽  
Author(s):  
H S Basu ◽  
L J Marton
Keyword(s):  
High Temperature ◽  
Melting Temperature ◽  
Conformational Changes ◽  
Thermus Thermophilus ◽  
Total Charge ◽  
Amino Groups ◽  
Carbon Chains ◽  
Naturally Occurring ◽  
Calf Thymus

We studied the effects of spermine, two naturally-occurring pentamines isolated from the thermophile Thermus thermophilus and one synthetic pentamine on the aggregation and ‘melting’ temperature of calf-thymus DNA and on the B-to-Z transition of poly(dG-me5dC). All pentamines caused aggregation of DNA at much lower concentrations than that of spermine. Concentrations that increased the melting temperature of DNA and induced the B-to-Z transition in poly(dG-me5dC) were different for each pentamine, but were comparable with the concentration of spermine needed to cause these effects. Our results suggest that both the total charge and the distance separating the charge, which is a function of the length of the carbon chains between amino groups, are important for the induction of conformational changes in DNA. The biological role of pentamines in T. thermophilus appears to be related to their ability to promote DNA condensation at high temperature.

Exploration of the binding interaction of a potential nervous system stimulant with calf-thymus DNA and dissociation of the drug–DNA complex by detergent sequestration

2015 ◽  
Vol 17 (27) ◽  
pp. 17699-17709 ◽  
Author(s):  
Pronab Kundu ◽  
Saptarshi Ghosh ◽  
Nitin Chattopadhyay
Keyword(s):  
Nervous System ◽  
Calf Thymus Dna ◽  
Binding Interaction ◽  
Calf Thymus ◽  
Dna Complex

The binding interaction of a potential nervous system stimulant with calf-thymus DNA has been divulged and dissociation of the drug–DNA complex has been achieved by the detergent sequestration method.

Conformational Changes of Aliphatic Ionenes in Water-Salt Solutions as a Factor Controlling Stability of Their Complexes with Calf Thymus DNA

2003 ◽  
Vol 36 (6) ◽  
pp. 2066-2071 ◽  
Author(s):  
A. N. Zelikin ◽  
A. A. Litmanovich ◽  
V. V. Paraschuk ◽  
A. V. Sybatchin ◽  
V. A. Izumrudov
Keyword(s):  
Conformational Changes ◽  
Calf Thymus Dna ◽  
Salt Solutions ◽  
Calf Thymus ◽  
Water Salt

scholarly journals Deciphering the interaction of flavones with calf thymus DNA and octamer DNA sequence (CCAATTGG)2

RSC Advances ◽  
2021 ◽  
Vol 11 (47) ◽  
pp. 29354-29371
Author(s):  
Shailendra Kumar ◽  
Maya S. Nair
Keyword(s):  
Dna Sequence ◽  
Conformational Changes ◽  
Calf Thymus Dna ◽  
Double Stranded Dna ◽  
Calf Thymus

Binding of flavones induce conformational changes in double stranded DNA.

Interaction of copper(II) ions with the daunomycin-calf thymus DNA complex

Biochemistry ◽  
1982 ◽  
Vol 21 (23) ◽  
pp. 5862-5870 ◽  
Author(s):  
M. Spinelli ◽  
James C. Dabrowiak
Keyword(s):  
Calf Thymus Dna ◽  
Calf Thymus ◽  
Dna Complex

scholarly journals Identification of Binding Mode of a Platinum (II) Complex, (DIP), and Calf Thymus DNA

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Nahid Shahabadi ◽  
Soheila Kashanian ◽  
Azadeh Fatahi
Keyword(s):  
Melting Temperature ◽  
Double Helix ◽  
Spectral Characteristics ◽  
Binding Mode ◽  
Calf Thymus Dna ◽  
Specific Viscosity ◽  
Pt Complex ◽  
Calf Thymus ◽  
Double Helix Structure ◽  
Dna Double Helix

The Pt(II) complex, PtCl2(DIP) (DIP = chelating dinitrogen ligand: 4,7-diphenyl-1,10-phenanthroline), was synthesized and characterized by elemental analysis (CHN) and NMR and UV-vis techniques. The binding of this complex to calf thymus DNA was investigated using various physicochemical methods such as spectrophotometric, circular dichroism, spectrofluorometric, melting temperature, and viscosimetric techniques. Upon addition of the complex, important changes were observed in the characteristic UV-Vis bands (hyperchromism) of calf thymus DNA (CT-DNA): increase in melting temperature, sharp increase in specific viscosity of DNA, and induced CD spectral changes. Also the fluorescence spectral characteristics and interaction of Pt complex with DNA have been studied. Pt bound to DNA showed a marked decrease in the fluorescence intensity. The results show that both the complex and the NR molecules can intercalate competitively into the DNA double-helix structure. The experimental results show that the mode of binding of the this complex to DNA is classical intercalation.

Carbon Chain Growth by Sequential Reactions of CO and CO2 with a Transition Metal Carbonyl Complex

2018 ◽  
Author(s):  
Richard Kong ◽  
Mark Crimmin
Keyword(s):  
Transition Metal ◽  
Metal Carbonyl ◽  
Carbon Chain ◽  
Chain Growth ◽  
Carbonyl Complex ◽  
Fischer Tropsch ◽  
Carbon Chains ◽  
Sequential Coupling ◽  
Energy Economy ◽  
Sequential Reactions

<i>The formation of carbon chains by the coupling of COx (X = 1 or 2) units on transition metals is a fundamental step relevant to Fischer-Tropsch catalysis. Fischer-Tropsch catalysis produces energy dense liquid hydrocarbons from synthesis gas (CO and H2) and has been a mainstay of the energy economy since its discovery nearly a century ago. Despite detailed studies aimed at elucidating the steps of catalysis, experimental evidence for chain growth (Cn to Cn+1 ; n > 2) from the reaction of CO with metal complexes is unprecedented. In this paper, we show that carbon chains can be grown from sequential reactions of CO or CO2 with a transition metal carbonyl complex. By exploiting the cooperative effect of transition and main group metals, we document the first example of chain propagation from sequential coupling of CO units (C1 to C3 to C4), along with the first example of incorporation of CO2 into the growing carbon chain.</i><br>

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