Exploration of the binding interaction of a potential nervous system stimulant with calf-thymus DNA and dissociation of the drug–DNA complex by detergent sequestration

2015 ◽  
Vol 17 (27) ◽  
pp. 17699-17709 ◽  
Author(s):  
Pronab Kundu ◽  
Saptarshi Ghosh ◽  
Nitin Chattopadhyay
Keyword(s):  
Nervous System ◽  
Calf Thymus Dna ◽  
Binding Interaction ◽  
Calf Thymus ◽  
Dna Complex

The binding interaction of a potential nervous system stimulant with calf-thymus DNA has been divulged and dissociation of the drug–DNA complex has been achieved by the detergent sequestration method.

Spectroscopic investigation of interaction of 6-methoxyflavanone and its β-cyclodextrin inclusion complex with calf thymus DNA

2012 ◽  
Vol 66 (8) ◽  
Author(s):  
Sameena Yousuf ◽  
Israel Enoch
Keyword(s):  
Fluorescence Intensity ◽  
Molecular Modelling ◽  
Calf Thymus Dna ◽  
Binding Interaction ◽  
Calf Thymus ◽  
Hyperchromic Effect ◽  
Voltammetric Studies ◽  
Presence And Absence ◽  
Different Characteristics ◽  
Cyclic Voltammetric Studies

AbstractThe interaction of 6-methoxyflavanone (6MF, 6-methoxy-2-phenyl-4H-1-benzopyran-4-one) with calf thymus DNA (ctDNA) was investigated by absorption spectroscopy, fluorescence spectroscopy, and cyclic voltammetry in the presence and absence of β-cyclodextrin (β-CD) acting as capping agent. Molecular modelling was used to optimise the study of 6MF-β-CD and 6MF-DNA interactions. Enhancement in the fluorescence intensity of 6MF was observed due to the formation of 1 : 1 complex with β-CD. In the presence and absence of DNA, 6MF showed different characteristics such as hyperchromic effect, red shift of absorption spectra and fluorescence quenching of 6MF due to binding between 6MF and ctDNA. The nature of the binding group was found to be different for the 6MF-ctDNA and 6MF-ctDNA-β-CD systems. An increase in fluorescence intensity was observed for the 6MF-ctDNA system while varying the concentration of β-CD due to encapsulation of a part of 6MF in cyclodextrin. The results are compatible with the possibility of the interaction of dihydrobenzopyran-4-one moiety of 6MF with ctDNA as well as with β-CD. Cyclic voltammetric studies confirmed the binding interaction between 6MF and ctDNA in the absence and presence of β-CD and molecular modelling explains the site of the interaction of 6MF with cyclodextrin and ctDNA.

Binding Interaction of Cationic Phenazinium Dyes with Calf Thymus DNA: A Comparative Study

2008 ◽  
Vol 112 (30) ◽  
pp. 9243-9249 ◽  
Author(s):  
Deboleena Sarkar ◽  
Paramita Das ◽  
Soumen Basak ◽  
Nitin Chattopadhyay
Keyword(s):  
Comparative Study ◽  
Calf Thymus Dna ◽  
Binding Interaction ◽  
Calf Thymus

Spectroscopic and viscometric elucidation of the interaction between a potential chloride channel blocker and calf-thymus DNA: the effect of medium ionic strength on the binding mode

2015 ◽  
Vol 17 (1) ◽  
pp. 483-492 ◽  
Author(s):  
Aniruddha Ganguly ◽  
Soumen Ghosh ◽  
Nikhil Guchhait
Keyword(s):  
Ionic Strength ◽  
Chloride Channel ◽  
Channel Blocker ◽  
Binding Mode ◽  
Calf Thymus Dna ◽  
Binding Interaction ◽  
Detailed Characterization ◽  
Calf Thymus ◽  
Chloride Channel Blocker

The present study demonstrates a detailed characterization of the binding interaction of a potential chloride channel blocker 9-methyl anthroate (9-MA) with calf-thymus DNA.

scholarly journals Effects of variation in the structure of spermine on the association with DNA and the induction of DNA conformational changes

1990 ◽  
Vol 269 (2) ◽  
pp. 329-334 ◽  
Author(s):  
H S Basu ◽  
H C A Schwietert ◽  
B G Feuerstein ◽  
L J Marton
Keyword(s):  
Melting Temperature ◽  
Conformational Changes ◽  
Carbon Chain ◽  
Calf Thymus Dna ◽  
Carbon Chains ◽  
Calf Thymus ◽  
Central Carbon ◽  
Induced Aggregation ◽  
Dna Complex ◽  
Spermine Analogues

The effects of spermine and spermine analogues on the B-Z transition of poly(dG-me5dC) and on the aggregation and ‘melting’ temperature of calf thymus DNA were studied by spectroscopic methods. The association constants of these polyamines with double- and single-stranded calf thymus DNA were calculated from their effects on the melting temperature. The effect of these compounds on the release of ethidium bromide (EB) from an EB-DNA complex were measured by a spectrofluorimetric method. This efficiency of the polyamine-induced B-Z transition strongly depended on the length of the central carbon chains of the compounds and on the functional groups attached to the carbon chains. Both the terminal primary amino groups and the length of the central carbon chain affected the aggregation of DNA. The affinity of the analogues for DNA increased as the number of n-butyl groups increased, but decreased with either an increase or a decrease in the length of the central carbon chain. The effect of spermine and spermine analogues on the release of EB from an EB-DNA complex did not always correlate with the affinities of analogues for calf thymus DNA. In particular, tetra-amines with more than one n-butyl group bound better to DNA than did spermine, but released bound EB and induced aggregation of DNA less well than did spermine. We postulate that either a bend and/or other localized conformational changes of DNA are responsible for the spermine-induced aggregation of DNA and the release of EB from the EB-DNA complex.

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