scholarly journals A novel preparative method for the isolation of avidin and riboflavin-binding glycoprotein from chicken egg-white by the use of high-performance liquid chromatography

1990 ◽  
Vol 265 (1) ◽  
pp. 301-304 ◽  
Author(s):  
V E Piskarev ◽  
A M Shuster ◽  
A G Gabibov ◽  
A G Rabinkov
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Organic Solvents ◽  
High Performance ◽  
Cation Exchanger ◽  
Preparative Method ◽  
Egg White ◽  
2So4 Concentration ◽  
Low Solubility ◽  
Binding Glycoprotein

A method for the rapid preparative isolation of highly purified avidin using h.p.l.c. on a powerful cation-exchanger TSK SP-5PW column has been developed. The method is based on the following properties of avidin: solubility at a high (NH4)2SO4 concentration, stability and relatively low solubility in organic solvents, as well as the strongly cationic nature of the molecule. Riboflavin-binding glycoprotein may be isolated as by-product.

scholarly journals Estradiol Solubility in Aqueous Systems: Effect of Ionic Concentrations, pH, and Organic Solvents

2013 ◽  
Vol 2013 ◽  
pp. 1-4 ◽  
Author(s):  
John E. A. Carter ◽  
Patrick M. Sluss
Keyword(s):  
Breast Cancer ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Human Serum ◽  
Clinical Significance ◽  
Organic Solvents ◽  
Diethyl Ether ◽  
High Performance ◽  
Reverse Phase ◽  
Concentration Levels

This study examined the effects of ionic strengths of NaCl (0.1, 0.3, and 1.0 M), pH (3, 7, and 11), and organic solvents (dichloromethane, diethyl ether, and methanol) on the extraction of estradiol at concentrations of 5.0 pg/mL in human serum. Methanol extracted almost 100% of the estradiol at a 5.0 pg/mL concentration, while ether and dichloromethane extracted only 73% or 70%, respectively, of the estradiol. The methanol extracted material was subjected to reverse phase high-performance liquid chromatography (HPLC) using 60% methanol and was found to elute at the same position as estradiol standard. These results suggest that methanol extraction of estradiol may prove useful in situations where estradiol occurs at concentration levels of ≥5.0 pg/mL, concentrations of great clinical significance in the detection and treatment of breast cancer.

Angiotensin I–Converting Enzyme Inhibitory Activity of Peptides Derived from Egg White Proteins by Enzymatic Hydrolysis

2004 ◽  
Vol 67 (9) ◽  
pp. 1914-1920 ◽  
Author(s):  
M. MIGUEL ◽  
I. RECIO ◽  
J. A. GÓMEZ-RUIZ ◽  
M. RAMOS ◽  
R. LÓPEZ-FANDIÑO
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Molecular Mass ◽  
Inhibitory Activity ◽  
High Performance ◽  
Egg White ◽  
Reversed Phase ◽  
Converting Enzyme ◽  
Ace Inhibitory Activity ◽  
Ace Inhibitory

The hydrolysis of crude egg white with pepsin, trypsin, and chymotrypsin produced peptides with angiotensin-converting enzyme (ACE) inhibitory properties. These peptides were mainly derived from the proteolysis of ovalbumin. The most active hydrolysates were obtained after treatment with pepsin (50% inhibitory concentration [IC50], 55.3 μg/ml), with the fraction having a molecular mass lower than 3,000 Da giving the highest ACE inhibitory activity (IC50, 34.5 μg/ml). Nine subfractions were collected from the fraction with a molecular mass lower than 3,000 Da using semipreparative reversed-phase high-performance liquid chromatography. Considerable ACE inhibitory activity (IC50 < 40 μg/ml) was found in three of them. These subfractions were analyzed by reversed-phase high-performance liquid chromatography–tandem mass spectrometry, and 14 peptides were identified. These sequences were synthesized, and their ACE inhibitory activities were measured. Among the identified peptides, two novel sequences with potent ACE inhibitory activity were found. The amino acid sequences of these inhibitors were identified as Arg-Ala-Asp-His-Pro-Phe-Leu and Tyr-Ala-Glu-Glu-Arg-Tyr-Pro-Ile-Leu and showed IC50 values of 6.2 and 4.7 μM, respectively.

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