scholarly journals Gas vesicle proteins

1989 ◽  
Vol 264 (2) ◽  
pp. 313-322 ◽  
Author(s):  
A E Walsby ◽  
P K Hayes
Keyword(s):  
Gas Vesicle Proteins ◽  
Vesicle Proteins

Heterologous expression of cyanobacterial gas vesicle proteins in Saccharomyces cerevisiae

2021 ◽  
pp. 2100059
Author(s):  
Harin Jung ◽  
Hua Ling ◽  
Yong Quan Tan ◽  
Nam‐Hai Chua ◽  
Wen Shan Yew ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Heterologous Expression ◽  
Gas Vesicle Proteins ◽  
Vesicle Proteins

scholarly journals Complexity of Gas Vesicle Biogenesis in Halobacterium sp. Strain NRC-1: Identification of Five New Proteins

2004 ◽  
Vol 186 (10) ◽  
pp. 3182-3186 ◽  
Author(s):  
Hem Dutt Shukla ◽  
Shiladitya DasSarma
Keyword(s):  
Self Assembly ◽  
Protein A ◽  
Sodium Dodecyl ◽  
Coiled Coil ◽  
Gas Vesicles ◽  
Immunoblotting Analysis ◽  
Large Gene ◽  
Vesicle Biogenesis ◽  
Gas Vesicle Proteins ◽  
Vesicle Proteins

ABSTRACT The genome of Halobacterium sp. strain NRC-1 contains a large gene cluster, gvpMLKJIHGFEDACNO, that is both necessary and sufficient for the production of buoyant gas-filled vesicles. Due to the resistance of gas vesicles to solubilization, only the major gas vesicle protein GvpA and a single minor protein, GvpC, were previously detected. Here, we used immunoblotting analysis to probe for the presence of gas vesicle proteins corresponding to five additional gvp gene products. Polyclonal antisera were raised in rabbits against LacZ-GvpF, -GvpJ, and -GvpM fusion proteins and against synthetic 15-amino-acid peptides from GvpG and -L. Immunoblotting analysis was performed on cell lysates of wild-type Halobacterium sp. strain NRC-1, gas vesicle-deficient mutants, and purified gas vesicles, after purification of LacZ fusion antibodies on protein A and β-galactosidase affinity columns. Our results show the presence of five new gas vesicle proteins (GvpF, GvpG, GvpJ, GvpL, and GvpM), bringing the total number of proteins identified in the organelles to seven. Two of the new gas vesicle proteins are similar to GvpA (GvpJ and GvpM), and two proteins contain predicted coiled-coil domains (GvpF and GvpL). GvpL exhibited a multiplet ladder on sodium dodecyl sulfate-polyacrylamide gels indicative of oligomerization and self-assembly. We discuss the possible functions of the newly discovered gas vesicle proteins in biogenesis of these unique prokaryotic flotation organelles.

scholarly journals Homology of Gas Vesicle Proteins in Cyanobacteria and Halobacteria

Microbiology ◽  
1984 ◽  
Vol 130 (10) ◽  
pp. 2709-2715 ◽  
Author(s):  
J. E. Walker ◽  
P. K. Hayes ◽  
J. Gjerde ◽  
A. E. Walsby
Keyword(s):  
Gas Vesicle Proteins ◽  
Vesicle Proteins

scholarly journals The homologies of gas vesicle proteins

1992 ◽  
Vol 138 (6) ◽  
pp. 1243-1250 ◽  
Author(s):  
A. E. Griffiths ◽  
A. E. Walsby ◽  
P. K. Hayes
Keyword(s):  
Gas Vesicle Proteins ◽  
Vesicle Proteins

scholarly journals Evidence for two different gas vesicle proteins and genes in Halobacterium halobium.

1988 ◽  
Vol 170 (4) ◽  
pp. 1746-1751 ◽  
Author(s):  
B Surek ◽  
B Pillay ◽  
U Rdest ◽  
K Beyreuther ◽  
W Goebel
Keyword(s):  
Halobacterium Halobium ◽  
Gas Vesicle Proteins ◽  
Vesicle Proteins

scholarly journals Interaction of Haloarchaeal Gas Vesicle Proteins Determined by Split-GFP

2018 ◽  
Vol 9 ◽  
Author(s):  
Kerstin Winter ◽  
Johannes Born ◽  
Felicitas Pfeifer
Keyword(s):  
Gas Vesicle Proteins ◽  
Vesicle Proteins

Regulation of gvp genes encoding gas vesicle proteins in halophilic Archaea

2008 ◽  
Vol 190 (3) ◽  
pp. 333-339 ◽  
Author(s):  
Sandra Scheuch ◽  
Larissa Marschaus ◽  
Simone Sartorius-Neef ◽  
Felicitas Pfeifer
Keyword(s):  
Halophilic Archaea ◽  
Genes Encoding ◽  
Gas Vesicle Proteins ◽  
Vesicle Proteins

Amyloid Precursor Protein Knockout Diminishes Synaptic Vesicle Proteins at the Presynaptic Active Zone in Mouse Brain

2014 ◽  
Vol 11 (10) ◽  
pp. 971-980 ◽  
Author(s):  
Melanie Laßek ◽  
Jens Weingarten ◽  
Amparo Acker-Palmer ◽  
Sandra Bajjalieh ◽  
Ulrike Muller ◽  
...  
Keyword(s):  
Amyloid Precursor Protein ◽  
Synaptic Vesicle ◽  
Mouse Brain ◽  
Active Zone ◽  
Precursor Protein ◽  
Presynaptic Active Zone ◽  
Vesicle Proteins
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