scholarly journals Oxidation of 3-amino-1-phenylprop-1-enes by monoamine oxidase and their use in a continuous assay of the enzyme

1988 ◽  
Vol 256 (3) ◽  
pp. 911-915 ◽  
Author(s):  
C H Williams ◽  
J Lawson ◽  
F R C Backwell
Keyword(s):  
Monoamine Oxidase ◽  
Absorption Coefficient ◽  
Mitochondrial Protein ◽  
Bovine Liver ◽  
Spectrophotometric Assay ◽  
Monoamine Oxidase A ◽  
Monoamine Oxidase B ◽  
High Absorption Coefficient ◽  
Continuous Assay ◽  
High Absorption

3-Amino-1-phenylprop-1-ene (cinnamylamine) and some derivatives were examined as substrates for monoamine oxidases A and B in mitochondria. All of the amines examined were readily oxidized by monoamine oxidase B but much less readily by monoamine oxidase A. E-Cinnamylamine was found to have Km 0.025 mM and Vmax. 3.9 nmol/min per mg of mitochondrial protein. Corresponding values with monoamine oxidase A were 0.026 mM and 0.85 nmol/min per mg respectively. Despite their different stereochemistry, E- and Z-N-methylcinnamylamines were almost equally effective as substrates for monoamine oxidase B. The characteristic u.v. absorbance and high absorption coefficient of cinnamaldehyde, the product produced by enzymic oxidation of cinnamylamine, is utilized in a sensitive continuous spectrophotometric assay for both enzymes in the rat and for the assay of a purified monoamine oxidase B from bovine liver.

scholarly journals Monoamine oxidase A from human placenta and monoamine oxidase B from bovine liver both have one FAD per subunit

1989 ◽  
Vol 260 (3) ◽  
pp. 725-729 ◽  
Author(s):  
W Weyler
Keyword(s):  
Amino Acid ◽  
Monoamine Oxidase ◽  
Bovine Liver ◽  
Spectrophotometric Titration ◽  
Monoamine Oxidase A ◽  
Monoamine Oxidase B ◽  
Enzyme Preparations ◽  
Function Mechanism ◽  
Upper Limit ◽  
Quantitative Amino Acid Analysis

I present the first clear evidence that the protein: FAD ratio in human monoamine oxidase A and bovine monoamine oxidase B has an upper limit of 65 kDa and 57 kDa per FAD, respectively. To now it had been assumed that the protein: FAD ratio was 100-120 kDa to 1 FAD and that there was one FAD per two subunits which were assumed to be of the same size. For the present work the purity of monoamine oxidase A and monoamine oxidase B was improved over that previously achieved. Protein was determined by quantitative amino acid analysis and FAD content was measured by spectrophotometric titration of SDS-denatured enzyme with NaS2O4 standardized against riboflavin. The cause of the previous misassignment of the protein: FAD ratio was judged as having been due to the use of impure enzyme preparations. Knowledge of the correct protein: FAD ratio is important in devising cloning strategies for this enzyme, in understanding its structure, function, mechanism, and in the studies of its biosynthesis.

scholarly journals Rational Design of Aqueous Conjugated Polymer Nanoparticles as Potential Theranostic Agents of Breast Cancer

2021 ◽  
Author(s):  
Panagiota Koralli ◽  
Spyridon Tsikalakis ◽  
Maria Goulielmaki ◽  
Stella Arelaki ◽  
Janina Müller ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Absorption Coefficient ◽  
Conjugated Polymer ◽  
Rational Design ◽  
Polymer Nanoparticles ◽  
Quantum Yields ◽  
Conjugated Polymer Nanoparticles ◽  
Theranostic Agents ◽  
High Absorption Coefficient ◽  
High Absorption

Conjugated polymer nanoparticles (CPNs) have emerged as a new promising class of cancer theranostic agents due to their desirable optical features, such as high absorption coefficient and photoluminescence quantum yields,...

Mechanical and optical behaviors: strain synergy effects in high temperature phase oxide of lead

2021 ◽  
Author(s):  
Qidong Kang ◽  
Fei Yang ◽  
Xinyu Zhang ◽  
Ziyu Hu
Keyword(s):  
High Temperature ◽  
Absorption Coefficient ◽  
Bulk Material ◽  
High Temperature Phase ◽  
Temperature Phase ◽  
Synergy Effects ◽  
High Absorption Coefficient ◽  
Very High ◽  
High Absorption

Since lead has a very high absorption coefficient μ, that the radiations from within the bulk material do not penetrate the layers. While, the oxygen and water (O2 and H2O)...

9.6%- Efficient All-Inorganic Sb2(S,Se)3 Solar Cell with MnS Hole-Transporting Laye

2022 ◽  
Author(s):  
Chen Qian ◽  
Jianjun Li ◽  
Kaiwen Sun ◽  
Chenhui Jiang ◽  
Jialiang Huang ◽  
...  
Keyword(s):  
Solar Cell ◽  
Absorption Coefficient ◽  
Light Harvesting ◽  
Low Cost ◽  
The Poor ◽  
Hole Transporting ◽  
Low Toxic ◽  
High Absorption Coefficient ◽  
Poor Stability ◽  
High Absorption

Antimony selenosulfide, Sb2(S,Se)3, has emerged as a promising light-harvesting material for its high absorption coefficient, suitable bandgap, low-toxic and low-cost constituents. However, the poor stability and high cost of widely...

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