scholarly journals Primary structure of the glycans from mouse serum and milk transferrins

1987 ◽  
Vol 247 (3) ◽  
pp. 571-578 ◽  
Author(s):  
Y Leclercq ◽  
G Sawatzki ◽  
J M Wieruszeski ◽  
J Montreuil ◽  
G Spik
Keyword(s):  
Primary Structure ◽  
Reductive Cleavage ◽  
Peptide Chain ◽  
Mouse Serum ◽  
Structural Determination ◽  
Methylation Analysis ◽  
Sugar Analysis ◽  
Alkaline Conditions ◽  
Mouse Milk ◽  
Fucose Residue

A ‘serotransferrin-like’ protein was purified from mouse milk. This serotransferrin cross-reacts immunologically with the serotransferrin isolated from mouse plasma and not with the mouse lactotransferrin (lactoferrin). Sugar analysis of the three transferrins, i.e. serotransferrin, milk ‘serotransferrin-like’ protein and lactotransferrin, revealed that the major difference between the glycan primary structure of mouse serotransferrin and those of mouse milk ‘serotransferrin-like’ protein and lactotransferrin concerns essentially the presence of one fucose residue in the last two proteins. For structural determination, the N-glycosidically linked glycans were released from the protein by a reductive cleavage of the oligosaccharide-protein linkage under strong alkaline conditions. The primary structure of the released oligosaccharide alditols was determined by methylation analysis and 400 MHz 1H-n.m.r. spectroscopy. The oligosaccharide alditols released from milk ‘serotransferrin-like’ protein and lactotransferrin were identical and were identified as disialylated biantennary glycans of the N-acetyl-lactosamine type with a fucose residue alpha-1,6-linked to the N-acetylglucosamine residue conjugated to the peptide chain and having the following primary structure: NeuAc(alpha 2-6)Gal(beta 1-4)GlcNAc(beta 1-2)Man(alpha 1-3)[NeuAc(alpha 2-6)Gal(beta 1-4)GlcNAc(beta 1-2)Man(alpha 1-6)]Man(beta 1-4)GlcNAc(beta 1-4)[Fuc(alpha 1-6)]GlcNAc(beta 1-N)Asn. The serotransferrin glycan has the same primary structure but is only partially fucosylated (10-15%).

Structural determination of the O-antigenic polysaccharide of enteropathogenicEscherichia coliO103:H2

2010 ◽  
Vol 56 (5) ◽  
pp. 367-372 ◽  
Author(s):  
Evgeny Vinogradov ◽  
Leann L. MacLean ◽  
Malcolm B. Perry
Keyword(s):  
Periodate Oxidation ◽  
Enterohemorrhagic Escherichia Coli ◽  
Structural Determination ◽  
Two Dimensional ◽  
Methylation Analysis ◽  
Polysaccharide Antigen ◽  
Antigenic Polysaccharide ◽  
Oxidation Degradation ◽  
C Nmr

The structure of the antigenic O-polysaccharide isolated from the lipopolysaccharide produced by enterohemorrhagic Escherichia coli O103:H2 was determined and shown to be composed of d-glucose (1 part), 2-acetamido-2-deoxy-d-glucose (2 parts), 2-acetamido-2-deoxy-d-galactose (1 part), and 3-deoxy-3-(R)-3-hydroxybutyramido-d-fucose (1 part). From the results of methylation analysis, Smith-type periodate oxidation degradation studies, and the use of one- and two-dimensional1H and13C NMR spectroscopy, the O-polysaccharide antigen was found to be an unbranched polymer of a repeating pentasaccharide unit having the following structure: →2)-β-d-Glcp-(1→2)-β-d-Fucp3NBu-(1→6)-α-d-GlcpNAc-(1→4)-α-d-GalpNAc-(1→3)-β-d-GlcpNAc-(1→, where Bu is (R)-3-hydroxybutyramido.

Prolonged antidiuretic action of vasopressin analogues in relation to their primary structure

1981 ◽  
Vol 46 (8) ◽  
pp. 1850-1855 ◽  
Author(s):  
Jana Škopková ◽  
Pavel Hrbas ◽  
Jiřina Slaninová ◽  
Tomislav Barth
Keyword(s):  
Amino Acids ◽  
Primary Structure ◽  
Peptide Chain ◽  
Amino Group ◽  
Prolonged Action ◽  
Disulphide Bridge ◽  
Vasopressin Analogues ◽  
Antidiuretic Action

The effectiveness of vasopressin analogues with prolonged antidiuretic action was related to their primary structure. Prolonged action was primarily due to the absence of the amino group of cysteine in position 1 of the peptide chain. The carba substitution of the disulphide bridge and introduction of basic homologous amino acids into position 8 contributed in differing degrees to the prolonged action of the analogues. [8-L-Norarginine]deamino vasopressin was the most potent of the analogues compared; its action was 10 times more prolonged than that of dDAVP.

scholarly journals Primary structure of arabinoxylans of ispaghula husk and wheat bran.

2003 ◽  
Vol 62 (1) ◽  
pp. 217-222 ◽  
Author(s):  
Sandra Edwards ◽  
Martin F. Chaplin ◽  
Anne D. Blackwood ◽  
Peter W. Dettmar
Keyword(s):  
Wheat Bran ◽  
Primary Structure ◽  
Glucuronic Acid ◽  
Structural Features ◽  
Enzymic Hydrolysis ◽  
Functional Bowel Disorders ◽  
Methylation Analysis ◽  
Beneficial Effects ◽  
Bowel Disorders ◽  
Water Holding

he primary structures of ispaghula husk and wheat bran were investigated in order to determine how and why these fibres are among the most beneficial dietary fibres. To this end, the polysaccharide preparations have been subjected to enzymic hydrolysis and methylation analysis. The results have shown ispaghula husk and wheat bran to be very-highly-branched arabinoxylans consisting of linear Β-D-→l(4)-linked xylopyranose →Xylp) backbones to which a-L-arabinofuranose →Araf) units are attached as side residues via α-→1(3) and a-→l(2) linkages. Other substituents identified as present in wheat bran include Β-D-glucuronic acid attached via the C→O)-2 position, and arabinose oligomers, consisting of two or more arabinofuranosyl residues linked via 1–2, 1–3, and 1–4 linkages. Ispaghula-husk arabinoxylan is more complex having additional side residues which include a-D-glucuronopyranose →GalAp)-→l→2(-linked-α-L-rhamnopyranose-(1→4)-β-D-Xylp, α-D-GalA/>-→l→3(-linked-a-L-Araf-→1)4(-p-D-Xylp, and <x-L-Araf-→1→3(-linked-p-D-Xylp-→1→4(-β-D-Xylp. The beneficial effects of increased faecal bulk and water-holding capacity are undoubtedly related to the structures of the arabinoxylans, with differences in their efficacy to treat various functional bowel disorders due to their specific structural features

The chemistry of Pinus radiata. VI. The water-soluble galactoglucomannan

1983 ◽  
Vol 36 (5) ◽  
pp. 947 ◽  
Author(s):  
DJ Brash
Keyword(s):  
Pinus Radiata ◽  
Small Degree ◽  
Degree Of Polymerization ◽  
Water Soluble ◽  
Structural Determination ◽  
Methylation Analysis ◽  
Degree Of Branching ◽  
Single Side ◽  
Complete Acid Hydrolysis

The two major components of the water-soluble polysaccharides extracted from the sapwood of Pinus radiata were a galactoglucomannan and an arabinogalactan. The galactoglucomannan had a number average degree of polymerization of approximately 30, and on complete acid hydrolysis it yielded D-mannose, D-galactose and D-glucose in the ratio 4.5 : 1.3 : 1.0. The structural determination of this polysaccharide by methylation analysis and 13C n.m.r. suggested that it is a linear glucomannan with α-D-galactopyranosyl residues as single side units. No evidence could be found of any branches in the polymer, although the possibility of a small degree of branching cannot be dismissed.

STUDIES ON THE PRIMARY STRUCTURE OF MOUSE SERUM AMYLOID P COMPONENT (SAP)

1982 ◽  
Vol 389 (1 C-Reactive Pr) ◽  
pp. 471-472 ◽  
Author(s):  
Jacqueline A. Taylor ◽  
C. J. Bruton ◽  
J. R. Clamp ◽  
F. C. de Beer ◽  
Marilyn L. Baltz ◽  
...  
Keyword(s):  
Primary Structure ◽  
Serum Amyloid ◽  
Mouse Serum ◽  
Amyloid P Component ◽  
Serum Amyloid P ◽  
Serum Amyloid P Component ◽  
Mouse Serum Amyloid ◽  
Amyloid P
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