scholarly journals Evidence for the presence of oligophosphoglyceroyl-ATP in rat offney

1986 ◽  
Vol 240 (2) ◽  
pp. 597-599 ◽  
Author(s):  
W L Hutchinson ◽  
P J Ratcliffe ◽  
J Mowbray
Keyword(s):  
Trichloroacetic Acid ◽  
Adenine Nucleotides ◽  
Purine Nucleotide ◽  
Purine Nucleotides ◽  
Nucleotide Content ◽  
Rat Hearts ◽  
Perfused Rat Hearts ◽  
Rapid Equilibrium

The inability to account for large systematic variations in total purine nucleotide content of perfused rat hearts led to the demonstration that the soluble adenine nucleotides are in rapid equilibrium with a highly phosphorylated hetero-oligomeric derivative whose structure appears to be 3-phospho[glyceroyl-gamma-triphospho-5′-adenosine-3′-3-phosp ho]4glyceroyl- gamma-triphospho-5′-adenosine [Hutchinson, Morris & Mowbray (1986) Biochem. J. 234, 623-627]. Analogous techniques to those used with hearts for specifically labelling tissue purine nucleotides followed by extration and purification of nucleotides from the trichloroacetic acid-precipitable fraction show the existence of a corresponding rapid equilibrium between ATP and an oligomeric tetraphosphoadenosine derivative in perfused kidneys.

scholarly journals The molecular structure of a rapidly formed oligomeric adenosine tetraphosphate derivative from rat heart

1986 ◽  
Vol 234 (3) ◽  
pp. 623-627 ◽  
Author(s):  
W L Hutchinson ◽  
P G Morris ◽  
J Mowbray
Keyword(s):  
Molecular Structure ◽  
Rat Heart ◽  
Chromatographic Analysis ◽  
Trichloroacetic Acid ◽  
Adenine Nucleotides ◽  
Specific Radioactivity ◽  
Rat Hearts ◽  
Perfused Rat Hearts ◽  
Rapid Equilibrium

The inability to account for large systematic variations with time in soluble adenine nucleotides in perfused rat hearts [Bates, Perrett & Mowbray (1978) Biochem. J. 176, 485-493; Mowbray, Bates & Perrett (1981) FEBS Lett. 131, 55-59; Mowbray, Perrett & Bates (1984) Int. J. Biochem. 16, 889-894] led us to show that the soluble nucleotides are in rapid equilibrium with some hitherto unrecognized trichloroacetic acid/methanol-precipitable highly phosphorylated heteropolymeric form [Mowbray, Hutchinson, Tibbs & Morris (1984) Biochem. J. 223, 627-632]. Selective digestion coupled to chromatographic analysis together with m.s. and 31P-n.m.r. spectrometry have now been used to show that the likely structure for a purified oligomer that is in specific-radioactivity equilibrium with tissue ATP is 3-phospho-[glyceroyl-gamma-triphosphoroyl-5′-adenosine-3′-3- phospho]4 glyceroyl-gamma-triphosphoroyl-5′-adenosine.

scholarly journals Systematic variations in the content of the purine nucleotides in the steady-state perfused rat heart. Evidence for the existence of controlled storage and release of adenine nucleotides

1978 ◽  
Vol 176 (2) ◽  
pp. 485-493 ◽  
Author(s):  
David J. Bates ◽  
David Perrett ◽  
John Mowbray
Keyword(s):  
Cyclic Amp ◽  
Rat Heart ◽  
Energy Demand ◽  
De Novo ◽  
Adenine Nucleotide ◽  
Adenine Nucleotides ◽  
Purine Nucleotide ◽  
Purine Nucleotides ◽  
Perfused Rat Heart ◽  
Adenine Nucleotide Pool

1. The contents of the major purine nucleotides in the isolated non-working perfused rat heart varied systematically during 80min of perfusion. In particular the amounts of ATP, ADP, GTP, cyclic AMP and cyclic GMP in the well-oxygenated myocardium showed changes ranging from 25 to 60% of the mean concentrations. The apparent periodicity was about 30min for some and about 60min for other nucleotides. 2. These data are in contrast with measurements of parameters reflecting heart performance, which remained constant over this period of perfusion. 3. The ATP/ADP ratio, the cyclic AMP content, the GTP content and the GTP/GDP ratio in the tissue bore a constant relationship to one another, and all showed the same temporal variation. 4. Increasing the energy demand on the heart by administration of bovine somatotropin (1μg/ml) tended to damp the variations, and generally lower the content of all the nucleotides. 5. The total extractable adenine nucleotide pool also showed systematic temporal variations of as much as 1.3μmol/g wet wt. of tissue within 10min. 6. These variations could not be accounted for as inter-conversion with adenosine, other purine nucleotides, nucleosides or purine-degradation products either in the tissue or in the perfusion medium. No evidence was found in this preparation of the purine nucleotide oscillations described by Lowenstein and his co-workers [see Tornheim & Lowenstein (1975) J. Biol. Chem.250, 6304–6314]. 7. Further, the pool size increases cannot be satisfactorily explained by either synthesis de novo or the breakdown of any purine macromolecular species in the cell. Thus it is suggested that an unsuspected substantial storage form of purine nucleotide may exist in heart.

Effects of antiarrhythmic drugs on the extraneuronal accumulation of isoprenaline in perfused rat hearts

1986 ◽  
Vol 334 (2) ◽  
pp. 145-148 ◽  
Author(s):  
Kazumi Sono ◽  
Yoshinobu Akimoto ◽  
Kazuyoshi Kurahashi ◽  
Motohatsu Fujiwara
Keyword(s):  
Antiarrhythmic Drugs ◽  
Rat Hearts ◽  
Perfused Rat Hearts
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