In perfused rat hearts ischaemia promotes the reversible conversion of appreciable quantities of soluble adenine nucleotides to a stable trichloroacetic acid-precipitable form

1981 ◽  
Vol 1 (11) ◽  
pp. 857-863 ◽  
Author(s):  
W. L. Hutchinson ◽  
D. J. Bates ◽  
D. Perrett ◽  
J. Mowbray
Keyword(s):  
Trichloroacetic Acid ◽  
Adenine Nucleotides ◽  
Rat Hearts ◽  
Perfused Rat Hearts

scholarly journals Evidence for the presence of oligophosphoglyceroyl-ATP in rat offney

1986 ◽  
Vol 240 (2) ◽  
pp. 597-599 ◽  
Author(s):  
W L Hutchinson ◽  
P J Ratcliffe ◽  
J Mowbray
Keyword(s):  
Trichloroacetic Acid ◽  
Adenine Nucleotides ◽  
Purine Nucleotide ◽  
Purine Nucleotides ◽  
Nucleotide Content ◽  
Rat Hearts ◽  
Perfused Rat Hearts ◽  
Rapid Equilibrium

The inability to account for large systematic variations in total purine nucleotide content of perfused rat hearts led to the demonstration that the soluble adenine nucleotides are in rapid equilibrium with a highly phosphorylated hetero-oligomeric derivative whose structure appears to be 3-phospho[glyceroyl-gamma-triphospho-5′-adenosine-3′-3-phosp ho]4glyceroyl- gamma-triphospho-5′-adenosine [Hutchinson, Morris & Mowbray (1986) Biochem. J. 234, 623-627]. Analogous techniques to those used with hearts for specifically labelling tissue purine nucleotides followed by extration and purification of nucleotides from the trichloroacetic acid-precipitable fraction show the existence of a corresponding rapid equilibrium between ATP and an oligomeric tetraphosphoadenosine derivative in perfused kidneys.

scholarly journals The molecular structure of a rapidly formed oligomeric adenosine tetraphosphate derivative from rat heart

1986 ◽  
Vol 234 (3) ◽  
pp. 623-627 ◽  
Author(s):  
W L Hutchinson ◽  
P G Morris ◽  
J Mowbray
Keyword(s):  
Molecular Structure ◽  
Rat Heart ◽  
Chromatographic Analysis ◽  
Trichloroacetic Acid ◽  
Adenine Nucleotides ◽  
Specific Radioactivity ◽  
Rat Hearts ◽  
Perfused Rat Hearts ◽  
Rapid Equilibrium

The inability to account for large systematic variations with time in soluble adenine nucleotides in perfused rat hearts [Bates, Perrett & Mowbray (1978) Biochem. J. 176, 485-493; Mowbray, Bates & Perrett (1981) FEBS Lett. 131, 55-59; Mowbray, Perrett & Bates (1984) Int. J. Biochem. 16, 889-894] led us to show that the soluble nucleotides are in rapid equilibrium with some hitherto unrecognized trichloroacetic acid/methanol-precipitable highly phosphorylated heteropolymeric form [Mowbray, Hutchinson, Tibbs & Morris (1984) Biochem. J. 223, 627-632]. Selective digestion coupled to chromatographic analysis together with m.s. and 31P-n.m.r. spectrometry have now been used to show that the likely structure for a purified oligomer that is in specific-radioactivity equilibrium with tissue ATP is 3-phospho-[glyceroyl-gamma-triphosphoroyl-5′-adenosine-3′-3- phospho]4 glyceroyl-gamma-triphosphoroyl-5′-adenosine.

Effects of antiarrhythmic drugs on the extraneuronal accumulation of isoprenaline in perfused rat hearts

1986 ◽  
Vol 334 (2) ◽  
pp. 145-148 ◽  
Author(s):  
Kazumi Sono ◽  
Yoshinobu Akimoto ◽  
Kazuyoshi Kurahashi ◽  
Motohatsu Fujiwara
Keyword(s):  
Antiarrhythmic Drugs ◽  
Rat Hearts ◽  
Perfused Rat Hearts

Translocation of G-Protein β3 subunit from the cytosol pool to the membrane pool by β1-Adrenergic receptor stimulation in perfused rat hearts

1999 ◽  
Vol 58 (9) ◽  
pp. 1497-1500 ◽  
Author(s):  
Ken Kageyama ◽  
Takeshi Murakami ◽  
Kenji Iizuka ◽  
Kumi Sato ◽  
Kazuo Ichihara ◽  
...  
Keyword(s):  
G Protein ◽  
Adrenergic Receptor ◽  
Receptor Stimulation ◽  
Rat Hearts ◽  
Perfused Rat Hearts
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