scholarly journals Milk composition of rats feeding restricted litters

1986 ◽  
Vol 233 (3) ◽  
pp. 917-919 ◽  
Author(s):  
M R Grigor ◽  
J Allan ◽  
A Carne ◽  
J M Carrington ◽  
A Geursen
Keyword(s):  
Fatty Acid ◽  
Dna Content ◽  
Total Protein ◽  
Milk Composition ◽  
Mammary Tissue ◽  
Lipogenic Enzymes ◽  
Milk Samples ◽  
Specific Activities ◽  
Fatty Acid Compositions

Milk samples were taken from rats feeding ten pups and from both the suckled and non-suckled glands of rats feeding two pups. The lipid, protein and lactose concentrations were similar in the milks from the secreting glands, but the fluid from the non-suckled glands contained less lactose and lipid but significantly higher total protein and transferrin concentrations. The fatty acid compositions of the milk from the three sources were very similar. The mammary tissue from the rats feeding ten pups had a higher DNA content/g wet wt. than either the suckled or non-suckled mammary tissue of the rats feeding two pups. The specific activities of several lipogenic enzymes were significantly lower in the non-suckled mammary tissue.

scholarly journals Regulation of lipogenic capacity in lactating rats

1982 ◽  
Vol 208 (3) ◽  
pp. 611-618 ◽  
Author(s):  
M R Grigor ◽  
A Geursen ◽  
M J Sneyd ◽  
S M Warren
Keyword(s):  
Mammary Gland ◽  
Fatty Acid ◽  
Malic Enzyme ◽  
Fatty Acid Synthase ◽  
Specific Activity ◽  
Mammary Glands ◽  
Lipogenic Enzymes ◽  
Pregnancy And Lactation ◽  
Specific Activities

1. The rate of mammary-gland lipogenesis measured in vivo from 3H2O was suppressed after decreasing the milk demand by decreasing the number of pups from ten to two or three, as well as by giving diets containing lipid [Grigor & Warren (1980) Biochem. J. 188, 61-65]. 2. The specific activities of the lipogenic enzymes fatty acid synthase, glucose 6-phosphate dehydrogenase and ‘malic’ enzyme increased between 6- and 10-fold in the mammary gland and between 2- and 3-fold in the livers during the first 10 days of lactation. The increases in specific activity coupled with the doubling of liver mass which occurred during pregnancy and lactation resulted in considerable differences in total liver activities when compared with virgin animals. 3. Although consumption of a diet containing 20% peanut oil suppressed the activities of the three lipogenic enzymes in the livers, only the ‘malic’ enzyme was affected in the mammary glands. 4. In contrast, decreased milk demand did not affect the specific activities of any of the liver enzymes, whereas it resulted in suppression of all three lipogenic enzymes of the mammary glands. There was no effect on either the cytoplasmic malate dehydrogenase or the lactate dehydrogenase of the mammary gland. 5. In all the experiments performed, the activity of the fatty acid synthase correlated with the amount of material precipitated by the rabbit antibody raised against rat fatty acid synthase.

Effect of mid-line or low-line milking systems on lipolysis and milk composition in dairy goats

2018 ◽  
Vol 156 (6) ◽  
pp. 848-854
Author(s):  
M. C. Beltrán ◽  
A. Manzur ◽  
M. Rodríguez ◽  
J. R. Díaz ◽  
C. Peris
Keyword(s):  
Fatty Acid ◽  
Free Fatty Acid ◽  
Milk Composition ◽  
Crossover Design ◽  
Dairy Goat ◽  
Dairy Goats ◽  
Milk Samples ◽  
Commercial Farms ◽  
Milk Storage ◽  
Enzymatic Coagulation

AbstractTwo experiments were carried out to investigate how milking in mid-line (ML) affects the lipolysis level and milk composition in goat livestock, in comparison with low-line (LL) milking. The first experiment took place, in triplicate, on an experimental farm. For each replicate, a crossover design (62 goats, two treatments, ML and LL, in two periods each lasting 4 days) was used. Milk samples were taken daily at 0 and 24 h after milking. In the first experimental replicate, some enzymatic coagulation cheeses were made, which were assessed by a panel of tasters at 50 and 100 days of maturation. In the second experiment, the lipolysis level and composition of tank milk from 55 commercial dairy goat farms (25 ML and 30 LL) were analysed, in milk samples taken in three different weeks. The results of the first experiment showed that ML milking increased free fatty acid (FFA) concentration in raw goat's milk significantly (0.71 v. 0.40 mmol/l, respectively). However, in the milk samples taken from commercial farms the FFA concentration remained unaffected by the milking pipeline height (0.59 v. 0.58 mmol/l for ML and LL, respectively). No significant differences were found in the milk composition, nor in the sensory characteristics in the cured cheeses, which suggests that factors other than the milkline height are able to influence the level of lipolysis under commercial conditions. Therefore, ML milking should not be discouraged, provided that the correct functioning and management of the milking operation and milk storage on the farm is guaranteed.

scholarly journals A Comparison of Changes in the Fatty Acid Profile of Human Milk of Spanish Lactating Women during the First Month of Lactation Using Gas Chromatography-Mass Spectrometry. A Comparison with Infant Formulas

Nutrients ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 3055 ◽  
Author(s):  
Silvia Sánchez-Hernández ◽  
Adelaida Esteban-Muñoz ◽  
Rafael Giménez-Martínez ◽  
María José Aguilar-Cordero ◽  
Beatriz Miralles-Buraglia ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Fatty Acid ◽  
Human Milk ◽  
Milk Composition ◽  
Gas Chromatography Mass Spectrometry ◽  
Infant Formulas ◽  
Mature Milk ◽  
Lactating Women ◽  
Milk Samples

Breastfeeding is the ideal way to provide infants with the nutrients they need for healthy growth and development. Milk composition changes throughout lactation, and fat is one of the most variable nutrients in human milk. The aim of this study was to determine the main differences between the fatty acid (FA) profile of human milk samples (colostrum, transitional, and mature milk group) and infant formulas. Human milk samples were provided by lactating women from Granada. Moreover, different commercial infant formulas were analyzed. FAs were determined using gas chromatography coupled with mass spectrometry. According to the results, oleic acid was the predominant monounsaturated fatty acid (41.93% in human milk and 43.53% in infant formulas), while palmitic acid was the most representative saturated fatty acid (20.88% in human milk and 23.09% in infant formulas). Significant differences were found between human milk groups and infant formulas, mainly in long-chain polyunsaturated FAs (LC-PUFAs). The content of araquidonic acid (AA) and docoxahexaenoic acid (DHA) was higher in human milk (0.51% and 0.39%, respectively) than in infant formulas (0.31% and 0.22%, respectively). Linoleic acid (LA) percentage (15.31%) in infant formulas was similar to that found in human milk (14.6%). However, α-linolenic acid (ALA) values were also much higher in infant formulas than in human milk (1.64% and 0.42%, respectively).

Fatty-acid synthesis in the echinoderms: Asterias rubens, Echinus esculentus and Holothuria forskali

1968 ◽  
Vol 48 (2) ◽  
pp. 521-533 ◽  
Author(s):  
W. V. Allen
Keyword(s):  
Fatty Acid ◽  
Acid Synthesis ◽  
Gas Liquid Chromatography ◽  
Asterias Rubens ◽  
Phospholipid Classes ◽  
Tissue Lipids ◽  
Specific Activities ◽  
Branched Chain ◽  
Layer Chromatography ◽  
Fatty Acid Compositions

The fatty-acid compositions of tissue lipids of Asterias rubens L., Echinus esculentus L. and Holothuria forskali Delia Chiaje were determined by means of gas-liquid chromatography. Considerable proportions of branched-chain, normal odd-numbered and eicosatetraenoic acids occur in the latter two animals. Tissues of all three animals were incubated with 1−14C-acetate. The relative specific activities of fatty-acid fractions isolated by thin-layer chromatography upon AgNO3-impregnated silica gel G fell in the order: saturates > monoenes — dienes > polyenes. The fatty-acid compositions of the alkoxydiglyceride, triglyceride and phospholipid classes of A. rubens hepatic caecal lipid were also determined.

scholarly journals Studies on acetyl-CoA carboxylase and fatty acid synthase from rat mammary gland and mammary tumours

1982 ◽  
Vol 208 (2) ◽  
pp. 443-452 ◽  
Author(s):  
P M Ahmad ◽  
D S Feltman ◽  
F Ahmad
Keyword(s):  
Mammary Gland ◽  
Fatty Acid ◽  
Fatty Acid Synthase ◽  
Mammary Tissue ◽  
Acetyl Coa Carboxylase ◽  
Lipogenic Enzymes ◽  
Acetyl Coa ◽  
Rat Mammary Gland ◽  
Mammary Gland Differentiation ◽  
Mammary Adenocarcinomas

The activities of two lipogenic enzymes, acetyl-CoA carboxylase and fatty acid synthase, were determined in two transplantable mammary adenocarcinomas (13762 and R3230AC) carried by non-pregnant, pregnant and lactating rats, and in mammary tissue of control animals (non-tumour-carrying) of comparable physiological states. During mammary-gland differentiation of control or tumour-carrying animals, the activities of acetyl-CoA carboxylase and fatty acid synthase in the lactating gland increased by about 40-50-fold over the values found in non-pregnant animals. On the other hand, in tumours carried by lactating dams there were only modest increases (1.5-2-fold) in acetyl-CoA carboxylase and fatty acid synthase compared with the neoplasms carried by non-pregnant animals. On the basis of the Km values for different substrates and immunodiffusion and immunotitration data, the fatty acid synthase of neoplastic tissues appeared to be indistinguishable from the control mammary-gland enzyme. However, a comparison of the immunotitration and immunodiffusion experiments indicated that the mammary-gland acetyl-CoA carboxylase might differ from the enzyme present in mammary neoplasms.

scholarly journals Correlation between Milk Bacteriology, Cytology and Mammary Tissue Histology in Cows: Cure from the Pathogen or Recovery from the Inflammation

Pathogens ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 364 ◽  
Author(s):  
Gabriel Leitner ◽  
Shlomo E. Blum ◽  
Oloeg Krifuks ◽  
Nir Edery ◽  
Uzi Merin
Keyword(s):  
Mononuclear Cells ◽  
Milk Composition ◽  
The Other ◽  
Mammary Tissue ◽  
Rt Pcr ◽  
Bacterial Isolation ◽  
Milk Samples ◽  
Fat Globules ◽  
Tissue Histology ◽  
The Individual

The aim of the current study was to verify the existence of a significant correlation between bacterial isolation (or not) and mammary gland inflammation, using traditional bacterial culturing and PCR, milk leucocytes distributions, and tissue histology. Twenty-two cows were tested at the level of the individual gland for bacteriological culture and real-time PCR (RT-PCR), milk composition, somatic cells count (SCC), and cell differentiation. Post-slaughter samples of teat-ends and mammary tissues were tested for histology and bacteriology by RT-PCR. The 88 glands were assigned to either outcome: 1. Healthy—no inflammation and no bacterial finding (NBF) (n = 33); 2. Inflammation and NBF (n = 26); 3. Inflammation and intra-mammary infection (n = 22) with different bacteria. Bacteriology of milk samples and that of the RT-PCR showed 91.4% agreement. In the lobule’s tissues of healthy glands, ~50% were milk producers and the other glands had dry areas with increased fat globules with a low number of leukocytes. In contrast, ~75% of the infected glands were identified as inflamed, but with no isolation of bacteria. Infiltration of mononuclear cells and neutrophils into the connective tissue was observed but not in the lobule’s lumen. In summary, the study confirms that not every mastitis/inflammation is also an infection.

scholarly journals Exploring Dry-Film FTIR Spectroscopy to Characterize Milk Composition and Subclinical Ketosis throughout a Cow’s Lactation

Foods ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2033
Author(s):  
Amira Rachah ◽  
Olav Reksen ◽  
Valeria Tafintseva ◽  
Felicia Judith Marie Stehr ◽  
Elling-Olav Rukke ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Partial Least Squares Regression ◽  
Principal Component ◽  
Milk Composition ◽  
Dietary Energy ◽  
Least Squares Regression ◽  
Subclinical Ketosis ◽  
Milk Samples ◽  
Fa Composition ◽  
Dry Film

The use of technologies for measurements of health parameters of individual cows may ensure early detection of diseases and maximization of individual cow and herd potential. In the present study, dry-film Fourier transform infrared spectroscopy (FTIR) was evaluated for the purpose of detecting and quantifying milk components during cows’ lactation. This was done in order to investigate if these systematic changes can be used to identify cows experiencing subclinical ketosis. The data included 2329 milk samples from 61 Norwegian Red dairy cows collected during the first 100 days in milk (DIM). The resulting FTIR spectra were used for explorative analyses of the milk composition. Principal component analysis (PCA) was used to search for systematic changes in the milk during the lactation. Partial least squares regression (PLSR) was used to predict the fatty acid (FA) composition of all milk samples and the models obtained were used to evaluate systematic changes in the predicted FA composition during the lactation. The results reveal that systematic changes related to both gross milk composition and fatty acid features can be seen throughout lactation. Differences in the predicted FA composition between cows with subclinical ketosis and normal cows, in particular C14:0 and C18:1cis9, showed that dietary energy deficits may be detected by deviations in distinct fatty acid features.

scholarly journals INCLUSION OF PROTECTED FAT IN DIETS ON THE MILK PRODUCTION AND COMPOSITION OF SAANEN GOATS

2015 ◽  
Vol 39 (2) ◽  
pp. 164-172 ◽  
Author(s):  
Bruna Susan de Labio Molina ◽  
Claudete Regina Alcalde ◽  
Bruna Hygino ◽  
Sérgio Mangano de Almeida Santos ◽  
Ludmila Couto Gomes ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Milk Production ◽  
Feed Efficiency ◽  
Dry Matter ◽  
Latin Square ◽  
Milk Composition ◽  
Dry Matter Intake ◽  
Milk Fatty Acid ◽  
Maximum Point ◽  
Milk Samples

According on the fat source included in the diets for goats, the milk production can be increased and milk fatty acid composition can be improved. This study was conducted to evaluate the inclusion of protected fat (CSFA - calcium salts of fatty acids) in the diets of lactating Saanen goats on milk production and composition. Five Saanen goats (63.23 ± 9.08 kg body weight and 105 ± 3 days of lactation) were distributed in a Latin square design (5 × 5) with five diets: control (without the addition of CSFA) and the other diets with: 6.25, 12.50, 18.75 and 25.0 g CSFA per kg dry matter (g/kg DM), respectively. Dry matter intake (DMI) and milk production (MP) were evaluated. Milk samples were collected to determine milk composition and fatty acid profile. Feed efficiency (FE) was estimated. The supplementation of CSFA up to 25 g/kg DM did not influence the DMI, MP and FE. Milk components production (g/d) was not influenced by the inclusion CSFA in the diet; however, there was a linear reduction of 0.87 g protein per kg of milk for each 1% CSFA included in the diet. The concentration of linolenic acid (18:3n-3) in milk was improved with a maximum point of 24 g of CSFA per kg DM. CSFA can be included in the diets of lactating Saanen goats up to 25 g/kg DM with no interference on DMI and MP.

scholarly journals Importance of 3-Hydroxy Fatty Acid Composition of Lipopeptides for Biosurfactant Activity

2005 ◽  
Vol 71 (12) ◽  
pp. 7690-7695 ◽  
Author(s):  
Noha H. Youssef ◽  
Kathleen E. Duncan ◽  
Michael J. McInerney
Keyword(s):  
Fatty Acids ◽  
Bacillus Subtilis ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Multiple Regression ◽  
Acid Composition ◽  
Oil Recovery ◽  
Multiple Regression Model ◽  
Specific Activities ◽  
Fatty Acid Compositions

ABSTRACT Biosurfactant production may be an economic approach to improving oil recovery. To obtain candidates most suitable for oil recovery, 207 strains, mostly belonging to the genus Bacillus, were tested for growth and biosurfactant production in medium with 5% NaCl under aerobic and anaerobic conditions. All strains grew aerobically with 5% NaCl, and 147 strains produced a biosurfactant. Thirty-five strains grew anaerobically with 5% NaCl, and two produced a biosurfactant. In order to relate structural differences to activity, eight lipopeptide biosurfactants with different specific activities produced by various Bacillus species were purified by a new protocol. The amino acid compositions of the eight lipopeptides were the same (Glu/Gln:Asp/Asn:Val:Leu, 1:1:1:4), but the fatty acid compositions differed. Multiple regression analysis showed that the specific biosurfactant activity depended on the ratios of both iso to normal even-numbered fatty acids and anteiso to iso odd-numbered fatty acids. A multiple regression model accurately predicted the specific biosurfactant activities of four newly purified biosurfactants (r 2 = 0.91). The fatty acid composition of the biosurfactant produced by Bacillus subtilis subsp. subtilis strain T89-42 was altered by the addition of branched-chain amino acids to the growth medium. The specific activities of biosurfactants produced in cultures with different amino acid additions were accurately predicted by the multiple regression model derived from the fatty acid compositions (r 2 = 0.95). Our work shows that many strains of Bacillus mojavensis and Bacillus subtilis produce biosurfactants and that the fatty acid composition is important for biosurfactant activity.

scholarly journals The variation of breast milk composition during the lactation stage for mothers from the Csík region

2010 ◽  
pp. 93-98
Author(s):  
Szidónia Salamon ◽  
Rozália Veronika Salamon ◽  
János Csapó
Keyword(s):  
Fatty Acid ◽  
Breast Milk ◽  
Acid Content ◽  
Fatty Acid Content ◽  
Zinc Content ◽  
Sampling Period ◽  
Milk Composition ◽  
Milk Samples ◽  
Lactation Stage ◽  
Essential Components

The aim of our research was the examination of breast-milk composition from mothers living in the Csík region and to follow their milk composition variations during lactation. In this article we present the results obtained from three mothers, paying particular attention to essential components. The breast milk samples were collected from healthy mothers with similar habits and age. The milk samples were collected with a hand pump at thesame time after the feeding. The sampling period was from day 5 to the 14th weeks of lactation. The nutrition of mothers was  recorded on a questionnaire, completed by the mothers themselves. Comparing our experimental results with data in the literature it was concluded that the milk protein content was very similar to the milk of mothers from other European countries, and is decreased during lactation.The total saturated fatty acid content was lower, but the palmitic acid content was slightly higher. Regarding the essential fatty acid composition the linoleic- and the arachidonic acid contents were appropriate from a nutritional point of view. The linolenic acid was present in lower amounts, but the docosahexanoic acid was almost undetectable.The iron and manganese contents were in good agreemen with published results, but the zinc content of the breast milk was lower and its copper content was higher. These differences in milk composition can be explained by the different nutritional habits of our subjects. 

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