scholarly journals UDP-glucosamine as a substrate for dolichyl monophosphate glucosamine synthesis

1986 ◽  
Vol 233 (3) ◽  
pp. 749-754 ◽  
Author(s):  
W McDowell ◽  
G Weckbecker ◽  
D O R Keppler ◽  
R T Schwarz
Keyword(s):  
Chick Embryo ◽  
Rat Liver ◽  
Deae Cellulose ◽  
Water Soluble ◽  
Soluble Product ◽  
Mild Acid Hydrolysis ◽  
Nucleotide Analogue ◽  
Embryo Cells ◽  
Glucose Synthesis ◽  
Mild Acid

The sugar nucleotide analogue UDP-glucosamine was found to function as a sugar donor in microsomal preparations of both chick-embryo cells and rat liver, yielding dolichyl monophosphate glucosamine (Dol-P-GlcN). This was characterized by t.l.c. and retention by DEAE-cellulose. Glucosamine was the only water-soluble product released on mild acid hydrolysis. Dol-P-GlcN did not serve as substrate by transferring its glucosamine moiety to dolichol-linked oligosaccharide. Competition experiments between UDP-[3H]glucose and UDP-glucosamine showed Dol-P-[3H]glucose synthesis to be depressed by 56 or 73% in microsomes from chick-embryo cells and rat liver respectively. The concentrations of the UDP-sugars in this experiment were comparable with those occurring in galactosamine-metabolizing liver. These findings suggest that Dol-P-GlcN, formed as a metabolite of D-galactosamine, may interfere with Dol-P-dependent reactions.

Pigments of marine animals. XI. Angular naphthopyrones from the crinoid Comanthus parvicirrus timorensis

1971 ◽  
Vol 24 (7) ◽  
pp. 1487 ◽  
Author(s):  
IR Smith ◽  
MD Sutherland
Keyword(s):  
Methyl Ether ◽  
Dry Weight ◽  
Water Soluble ◽  
Spectral Studies ◽  
Mild Acid Hydrolysis ◽  
Marine Animals ◽  
Yellow Colour ◽  
Methoxy Derivative ◽  
Colour Variant ◽  
Mild Acid

Green specimens of the comatulid crinoid, Comanthus parvicirrus timorensis J. Muller, yield to acetone three yellow water-soluble colouring matters, comaparvin sulphate, 6-methoxycomaparvin sulphate, and 6-methoxycomaparvin 5-methyl ether sulphate in approximately 0.1 %, 0.7 %, and 0.7 % yield respectively of the dry weight of the animal, Mild acid hydrolysis yields the corresponding phenols, the structures of which have been deduced largely by spectral studies as very probably 5,8-dihydroxy-10-methoxy-2-n-propyl-4H-naphtho[1,2-b]pyran-4-one (1), the 6-methoxy derivative of (1), and the 6-methoxy methyl ether of (1) respectively. A yellow colour variant of the same species yielded the same colouring matters in slightly different proportions. The calcareous skeleton contains what are probably polyhydroxynaphthoquinones in combined form.

scholarly journals A mannosyl-carrier lipid of bovine adrenal meddulla and rat parotid

1975 ◽  
Vol 146 (3) ◽  
pp. 645-651 ◽  
Author(s):  
D A White ◽  
C J Waechter
Keyword(s):  
Acid Hydrolysis ◽  
Alkaline Hydrolysis ◽  
Deae Cellulose ◽  
Rapid Loss ◽  
Mild Acid Hydrolysis ◽  
Pig Liver ◽  
High Concentrations ◽  
Rat Parotid ◽  
Chloroform Methanol ◽  
Mild Acid

1. The transfer of mannose from GDP-(U-14-C)mannose into endogenous acceptors of bovine adrenal medullla and rat parotid was studied. The rapidly labelled product, a glycolipid, was partially purified and characterized. 2. It was stable to mild alkaline hydrolysis but yielded (14-C)mannose on mild acid hydrolysis. It co-chromatographed with mannosyl phosphoryl dolichol in four t.l.c. systems and on DEAE-cellulose acetate. Addition of dolichol phosphate or a dolichol phosphate-enriched fraction prepared from pig liver stimulated mannolipid synthesis. 3. The formation of mammolipid appeared reversible, since addition of GDP to a system synthesizing the mannolipid caused a rapid loss of label from the mannolipid. UDP-N-acetylglucosamine did not inhibit mannolipid synthesis except at high concentrations (2 mM), even though in the absence of GDP-mannose, N-acetylglucosamine was incorporated into a lipid having the properties of a glycosylated polyprenyl phosphate. 4. Mannose from GDP-mannose was also incorporated into two other acceptors, (2y being insoluble in chloroform-methanol (2:1, v/v) but soluble in choloroform-methanol-water (10:10:3, by vol.) and (ii) protein. These are formed much more slowly than the mannolipid. 5. Exogenous mannolipid served as a mannose donor for acceptors (i) and (ii), and it is suggested that transfer of mannose from GDP-mannose to mannosylated protein occurs via two intermediates, the mannolipid and acceptor (i).

scholarly journals Mild-acid hydrolysis of a native polysulfated fraction from Acanthophora muscoides generates sulfated oligosaccharides displaying in vitro thrombin generation inhibition

2016 ◽  
Vol 38 (1) ◽  
pp. 7 ◽  
Author(s):  
José Ariévilo Gurgel Rodrigues ◽  
Ismael Nilo Lino de Queiroz ◽  
Ana Luíza Gomes Quinderé ◽  
Norma Maria Barros Benevides ◽  
Ana Maria Freire Tovar ◽  
...  
Keyword(s):  
Acid Hydrolysis ◽  
Thrombin Generation ◽  
Deae Cellulose ◽  
Critical Time ◽  
Molecular Size ◽  
Resonance Structure ◽  
Nuclear Magnetic Resonance Structure ◽  
Mild Acid Hydrolysis ◽  
Mild Acid

A. muscoides (Rhodophyta) has three polysulfated fractions (-1, -2 and Am-3). Am-2 displayed anti-inflammation and serpin-independent anticoagulation effects; however, no effect of oligomers on thrombin-generation (TG) has been demonstrated. This study employed mild-acid hydrolysis to obtain low-molecular-size derivatives from Am-2 and compared in vitro inhibitory effects between intact Am-2 and its hydrolysates on a TG assay. The polysaccharidic extract was fractionated by DEAE-cellulose that revealed Am-2 eluted with 0.75-M NaCl containing sulfate (23%), hexoses (51%) and absence of proteins, and indicating, by one-dimension nuclear magnetic resonance, structure of galactan similar to that of the extract. The depolymerization with HCl (0.02 or 0.04-M, 60°C) for different times progressively reduced the charge density and the molecular-size of Am-2 based on electrophoresis in agarose and polyacrylamide gels, respectively, where at higher acid concentration and critical time up to 5h yielded fragment of  ̴ 14-kDa similar to that of unfractionated heparin (UHEP). Regarding the TG assay, intact Am-2 inhibited concentration-dependent intrinsic pathway, whereas its hydrolysates abolished it like UHEP, except the analog fragment (92.87% inhibition), when in 60-fold diluted human plasma using chromogenic method in a continuous system. The results reveal an alternative approach for the production of oligosaccharides from A. muscoides with TG inhibition. 

THE WATER-SOLUBLE POLYSACCHARIDES OF DERMATOPHYTES: III. A GALACTOMANNAN FROM TRICHOPHYTON INTERDIGITALE

1964 ◽  
Vol 42 (12) ◽  
pp. 2862-2871 ◽  
Author(s):  
F. Blank ◽  
M. B. Perry
Keyword(s):  
Formic Acid ◽  
Acid Hydrolysis ◽  
Copper Complex ◽  
Periodate Oxidation ◽  
Water Soluble ◽  
Mild Acid Hydrolysis ◽  
Trichophyton Interdigitale ◽  
Soluble Polysaccharide ◽  
Hydrolysis Of ◽  
Mild Acid

The water-soluble polysaccharide preparation from Trichophytoninterdigitale was fractionated to give two distinct galactomannans and a glucan. A galactomannan isolated via its insoluble copper complex had [α]D +75° (water) and was composed of D-galactose (12%) and D-mannose (88%). On periodate oxidation, the galactomannan consumed 1.73 mole periodate and released 0.67 mole formic acid and 0.12 mole formaldehyde per anhydrohexose unit. Hydrolysis of the methylated galactomannan gave 2,3,5,6-tetra-O-methyl-D-galactose (1 part), 2,3,4,6-tetra-O-methyl-D-mannose (1 part), 2,3,4-tri-O-methyl-D-mannose (4 parts), and3,4-di-O-methyl-D-mannose (2 parts). Mild acid hydrolysis of the galactomannan removed all the galactose residues, leaving a mannan having [α]D +84° (water) whose structure was analyzed by periodate oxidation and methylation techniques.

Morphological Examination of a Herpes-type Virus Indigenous for Tree Shrews

1970 ◽  
Vol 28 ◽  
pp. 160-161
Author(s):  
J. P. Brunschwig ◽  
R. M. McCombs ◽  
R. Mirkovic ◽  
M. Benyesh-Melnick
Keyword(s):  
Electron Microscopy ◽  
Soft Tissue ◽  
Chick Embryo ◽  
Soft Tissue Sarcoma ◽  
Cell Cultures ◽  
Tree Shrew ◽  
Type Virus ◽  
Morphological Examination ◽  
Tree Shrews ◽  
Embryo Cells

A new virus, established as a member of the herpesvirus group by electron microscopy, was isolated from spontaneously degenerating cell cultures derived from the kidneys and lungs of two normal tree shrews. The virus was found to replicate best in cells derived from the homologous species. The cells used were a tree shrew cell line, T-23, which was derived from a spontaneous soft tissue sarcoma. The virus did not multiply or did so poorly for a limited number of passages in human, monkey, rodent, rabbit or chick embryo cells. In the T-23 cells, the virus behaved as members of the subgroup B of herpesvirus, in that the virus remained primarily cell associated.

Amino acid sequence of cyanogen bromide fragment CB5 of human hemopexin

1989 ◽  
Vol 54 (3) ◽  
pp. 803-810 ◽  
Author(s):  
Ivan Kluh ◽  
Ladislav Morávek ◽  
Manfred Pavlík
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Acid Hydrolysis ◽  
Cyanogen Bromide ◽  
Polypeptide Chain ◽  
Amino Acid Residues ◽  
Mild Acid Hydrolysis ◽  
Methionine Residue ◽  
Cyanogen Bromide Fragment ◽  
Mild Acid

Cyanogen bromide fragment CB5 represents the region of the polypeptide chain of hemopexin between the fourth and fifth methionine residue (residues 232-352). It contains 120 amino acid residues in the following sequence: Arg-Cys-Ser-Pro-His-Leu-Val-Leu-Ser-Ala-Leu-Thr-Ser-Asp-Asn-His-Gly-Ala-Thr-Tyr-Ala-Phe-Ser-Gly-Thr-His-Tyr-Trp-Arg-Leu-Asp-Thr-Ser-Arg-Asp-Gly-Trp-His-Ser-Trp-Pro-Ile-Ala-His-Gln-Trp-Pro-Gln-Gly-Pro-Ser-Ala-Val-Asp-Ala-Ala-Phe-Ser-Trp-Glu-Glu-Lys-Leu-Tyr-Leu-Val-Gln-Gly-Thr-Gln-Val-Tyr-Val-Phe-Leu-Thr-Lys-Gly-Gly-Tyr-Thr-Leu-Val-Ser-Gly-Tyr-Pro-Lys-Arg-Leu-Glu-Lys-Glu-Val-Gly-Thr-Pro-His-Gly-Ile-Ile-Leu-Asp-Ser-Val-Asp-Ala-Ala-Phe-Ile-Cys-Pro-Gly-Ser-Ser-Arg-Leu-His-Ile-Met. The sequence was derived from the data on peptides prepared by cleavage of fragment CB5 by mild acid hydrolysis, by trypsin and chymotrypsin.

scholarly journals Incorporation of Leucine-C14 into a Virus-specific Protein in Homogenates of Chick Embryo Cells

1960 ◽  
Vol 235 (3) ◽  
pp. 660-664
Author(s):  
Gerald C. Mueller ◽  
Sigrid Von Zahn-Ullmann ◽  
Werner Schäfer
Keyword(s):  
Chick Embryo ◽  
Specific Protein ◽  
Embryo Cells
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