scholarly journals Leucine and tryptophan metabolism in rats

1985 ◽  
Vol 225 (2) ◽  
pp. 277-281 ◽  
Author(s):  
M Salter ◽  
D A Bender ◽  
C I Pogson
Keyword(s):  
Amino Acid ◽  
Membrane Transport ◽  
Liver Cells ◽  
Tryptophan Metabolism ◽  
Extreme Conditions ◽  
Isolated Liver Cells ◽  
Tryptophan Oxidation ◽  
Isolated Liver ◽  
Tryptophan Uptake

The rate of tryptophan metabolism in isolated liver cells from animals fed on a high-leucine diet was greater than for cells from control animals. Leucine inhibited tryptophan metabolism and tryptophan uptake in isolated liver cells, probably by competing for membrane transport. Leucine had no effect on tryptophan 2,3-dioxygenase in vitro. 4-Methyl-2-oxovalerate increased tryptophan oxidation in incubations containing albumin, by displacing bound tryptophan and increasing the availability of the amino acid to the cell. The results suggest that, under extreme conditions, when the availability of tryptophan is low, leucine may be pellagragenic.

scholarly journals The role of haem in the regulation of rat liver tryptophan metabolism

1986 ◽  
Vol 240 (1) ◽  
pp. 259-263 ◽  
Author(s):  
M Salter ◽  
C I Pogson
Keyword(s):  
Rat Liver ◽  
Intraperitoneal Injection ◽  
Liver Cells ◽  
Tryptophan Metabolism ◽  
Isolated Liver Cells ◽  
Tryptophan Oxidation ◽  
Isolated Liver

At saturating concentrations of tryptophan, the activity of tryptophan 2,3-dioxygenase was the same in isolated liver cells and in extracts with added haematin. Intraperitoneal injection of haematin did not increase tryptophan oxidation in livers subsequently perfused in situ. Preincubation of liver cells with physiological concentrations of tryptophan caused maximal saturation of tryptophan 2,3-dioxygenase with haem in liver cells. In cell-free extracts tryptophan 2,3-dioxygenase exhibited complex kinetics with haem. The results have important implications for the understanding of the role of haem in tryptophan metabolism.

Pathobiology and experimental therapeutics in hepatocellular cholestasis: lessons from the hepatocyte couplet model

2002 ◽  
Vol 102 (6) ◽  
pp. 603-614 ◽  
Author(s):  
Piotr MILKIEWICZ ◽  
Marcelo G. ROMA ◽  
Elwyn ELIAS ◽  
Roger COLEMAN
Keyword(s):  
Experimental Data ◽  
Intrahepatic Cholestasis ◽  
Liver Cells ◽  
Experimental Models ◽  
Experimental Therapeutics ◽  
Bile Formation ◽  
Isolated Liver Cells ◽  
Hepatocellular Cholestasis ◽  
Isolated Liver

Preparations of isolated liver cells, either freshly prepared or in culture, have been available for many years; however, because they lack the polarization of the cell in the tissue, their application to the study of processes involved in bile formation has been very limited. The hepatocyte couplet model offers a unique opportunity to study in vitro the intracellular processes involved: not only the physiology and pathophysiology of bile formation, but also the corresponding structural and molecular disturbances underlying different experimental models of cholestasis. In this review we describe the experimental data generated from this model in our laboratory over a period of years, including application of hepatocyte couplets to the study of cytoskeletal changes within the hepatocyte and mechanisms of hepatoprotection in experimentally induced, clinically relevant models of intrahepatic cholestasis.

In vitro metabolism of vitamin D3 by isolated liver cells

1977 ◽  
Vol 15 (3) ◽  
pp. 213-217 ◽  
Author(s):  
Joseph F. Reitano ◽  
Martha A. Reed ◽  
Patricia L. Rostron ◽  
Charles M. Intenzo ◽  
David M. Capuzzi
Keyword(s):  
Vitamin D3 ◽  
Liver Cells ◽  
In Vitro Metabolism ◽  
Isolated Liver Cells ◽  
Isolated Liver

scholarly journals Acceleration of gluconeogenesis from lactate by lysine (Short Communication)

1973 ◽  
Vol 134 (2) ◽  
pp. 671-672 ◽  
Author(s):  
Neal W. Cornell ◽  
Patricia Lund ◽  
Reginald Hems ◽  
Hans A. Krebs
Keyword(s):  
Amino Acid ◽  
Cyclic Amp ◽  
Short Communication ◽  
Liver Cells ◽  
Dibutyryl Cyclic Amp ◽  
Isolated Liver Cells ◽  
Isolated Liver

l-Lysine (2mm) causes an increase (mean 60%) in the rate of gluconeogenesis from lactate in isolated liver cells. The effect is of a catalytic nature. No other amino acid has the same effect, though ornithine is slightly active. The effect is additional to the stimulatory effects of oleate and of dibutyryl cyclic AMP.

scholarly journals Arachidonic acid synthesis studied in isolated liver cells

FEBS Letters ◽  
1981 ◽  
Vol 133 (2) ◽  
pp. 201-204 ◽  
Author(s):  
B.O. Christophersen ◽  
Jon Norseth
Keyword(s):  
Arachidonic Acid ◽  
Acid Synthesis ◽  
Liver Cells ◽  
Isolated Liver Cells ◽  
Isolated Liver
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