scholarly journals Inositol lipids, phosphatidate and diacylglycerol share stearoylarachidonoylglycerol as a common backbone in thrombin-stimulated human platelets

1984 ◽  
Vol 224 (3) ◽  
pp. 933-940 ◽  
Author(s):  
G Mauco ◽  
C A Dangelmaier ◽  
J B Smith
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Phosphatidic Acid ◽  
Human Platelets ◽  
Inositol Phospholipids ◽  
Inositol Lipids ◽  
Total Fatty Acids ◽  
Arachidonic Acids ◽  
Phosphatidylinositol 4

Gel-filtered human platelets were stimulated with 5i.u. of thrombin/ml for times up to 1 min. The fatty acid composition of inositol-containing phospholipids, phosphatidic acid and diacylglycerol was determined by g.l.c. in control and thrombin-stimulated platelet suspensions. Inositol phospholipids were found to have similar proportions of stearic and arachidonic acids, the sum of these representing 86.6% of the total fatty acids in phosphatidylinositol (PtdIns), 76.9% in phosphatidylinositol 4-phosphate (PtdIns4P) and 85.4% in phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2]. However, arachidonic and stearic acids were less abundant in phosphatidic acid (PtdA) and diacylglycerols in non-stimulated platelets. A transient decrease in the mass of PtdIns(4,5)P2 was observed after 5-10s of thrombin stimulation, followed by an increase after 30s. The amounts of PtdIns4P and PtdIns decreased throughout the experiment. A transient accumulation of stearoylarachidonoylglycerol was observed at 5s, whereas stearoylarachidonoylglycerol 3-phosphate (PtdA) was produced in increasing amounts throughout the experiment. The decrease in inositol-containing phospholipids was not fully compensated for by the production of diacylglycerol or PtdA [or PtdIns(4,5)P2] at 1 min. All the changes in inositol phospholipids, as well as those observed in diacylglycerols and PtdA, were due to a parallel reduction or increase in the contents of stearic and arachidonic acids, with a stoichiometry equal to 1. Taken together, this suggests an interconversion of all these lipids with the utilization of a common backbone, stearoylarachidonoylglycerol. The deacylation of this diacylglycerol could account for up to 4-5nmol of arachidonate/10(9) platelets after 1 min stimulation by thrombin.

Fatty Acid Composition of Nephtys incisa and Yoldia limatula

1973 ◽  
Vol 30 (2) ◽  
pp. 181-185 ◽  
Author(s):  
John W. Farrington ◽  
James G. Quinn ◽  
Wayne R. Davis
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Rhode Island ◽  
Acid Composition ◽  
Fatty Acid Distribution ◽  
Narragansett Bay ◽  
Total Fatty Acids ◽  
Polluted Station

Samples of the infaunal invertebrates Nephtys incisa and Yoldia limatula from Narragansett Bay, Rhode Island, have been analyzed for their fatty acid distribution. Based on total fatty acids, Yoldia contains 9–16% of an acid tentatively identified as 22:2. The ratios of 18:1/18:0 and 18:1/20:1 fatty acids of Nephtys from a polluted station in the bay are lower than the corresponding ratios for animals from relatively clean areas.

PSVI-21 Fatty acid composition of blood lipids in lambs of different genotypes in ontogeny

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 232-233
Author(s):  
Ekaterina Karpova ◽  
Ludmila Chizhova ◽  
Eugenia Surzhikova
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Growth And Development ◽  
Blood Lipids ◽  
Early Period ◽  
Gas Liquid Chromatography ◽  
Special Role ◽  
Arachidonic Acids

Abstract The early period of postnatal ontogeny of sheep is characterized by a variety of metabolic processes that result in the formation and consolidation of a certain type of metabolism. A special role is played by lipids, which together with proteins and carbohydrates constitute the bulk of organic compounds, cells and the organism as a whole. The purpose of this research was to study the polymorphism of CAST and GH genes as well as its association with the fatty acid composition of blood plasma lipids in lambs. The content of fatty acids was determined by gas-liquid chromatography in the form of methyl esters. From the data obtained, it follows that in all studied ontogeny periods the peripheral blood of sheep with CASTNN and CASTMM genotypes had a higher concentration of fatty acids such as palmitic, stearic, linoleic, and arachidonic acids. The content is as follows: 27.58, 45.40, 3.16, 0.28% vs. 26.21, 45.31, 3.62, 0.18% at the age of 2 months; 25.07, 43.25, 6.05, 1.74% vs. 23.24, 38.76, 6.49, 1.32% at the age of 4 months; 20.64, 22.26, 14.17, 3.38% vs. 17.64, 19.91, 15.07, 2.46% at the age of 8 months (P ˂ 0.05), (P ˂ 0.01). Studies of blood lipid composition of GHBBand GHAA genotype carriers showed that palmitic, stearic, linoleic, and arachidonic acids were dominant: 27.95, 27.96, 19.04% vs. 26.08, 23.88, 17.56% at the age of 2 months, 46.87, 39.87, 22.37% vs. 3.24, 6.87, 15.56% at the age of 4 months; 0.45, 1.68, 3.81% vs. 0.29, 1.29, 3.19% at the age of 8 months (P ˂ 0.05), (P ˂ 0.01). Thus, this study emphasizes that there is a close relationship between the ratio of fatty acids in total blood lipids in lambs and the intensity of growth and development, ensuring and controlling the direction of biochemical processes in the growth and development of young sheep.

scholarly journals Effects of feeding tuna oil on the lipid composition of pig spermatozoa and in vitro characteristics of semen

Reproduction ◽  
2001 ◽  
pp. 315-322 ◽  
Author(s):  
JA Rooke ◽  
CC Shao ◽  
BK Speake
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Basal Diet ◽  
Tuna Oil ◽  
Total Fatty Acids ◽  
Boar Semen ◽  
Boar Spermatozoa

The aim of the present study was to characterize the effects of feeding tuna oil on the lipid and fatty acid composition of boar spermatozoa and to relate changes in composition to boar semen characteristics. Ten boars were paired by age and allocated to one of two diets (five boars per diet). The diets, which were offered for 6 weeks, consisted of a basal diet that was either unsupplemented or supplemented with 30 g tuna oil kg(-1) diet. Adding tuna oil to the diet increased the ether extract concentration of the diets fed from 65 to 92 g kg(-1) dry matter and supplied 10.5 g long chain polyunsaturated (n-3) fatty acids per 100 g total fatty acids. There were no changes in semen fatty acid composition after 3 weeks of feeding tuna oil. However, after 5 and 6 weeks, the proportions (g per 100 g total fatty acids) of 22:6(n-3) in sperm phospholipid fatty acids were increased from 34.5 to 42.9 g by feeding tuna oil and 22:5(n-6) decreased from 29.8 to 17.9 g. No changes were observed in other sperm lipids or seminal plasma phospholipids as a result of the diets fed. Feeding tuna oil increased the proportion of spermatozoa with progressive motility and with a normal acrosome score and reduced the proportion of spermatozoa with abnormal morphologies.

Chloroplast Fatty Acid Composition in Mediterranean Populations of the Marine Chlorophyte, Anadyomene stellata

2000 ◽  
Vol 55 (7-8) ◽  
pp. 569-575 ◽  
Author(s):  
Debra L. Bemis ◽  
Vassilios Roussis ◽  
Constantinios Vagias ◽  
Robert S. Jacobs
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
Florida Keys ◽  
Mediterranean Populations ◽  
Total Fatty Acids ◽  
Of Greece

Abstract Chloroplasts isolated from three populations of the tropical marine Chlorophyte Anadyomene stellata collected off the coast of Greece were analyzed for their fatty acid composition. Following the preparation of fatty acid methyl esters, GC-MS (El) was utilized to identify the fatty acids present in each population. Including isomers, the fatty acid profile of all three algal populations was comprised of 19 fatty acids (4 saturated, 6 monounsaturated, 9 polyunsaturated) with palmitic acid present in the highest amounts (25-27% of total fatty acids). Analysis of variance revealed significant differences amongst the three populations in the percent of total fatty acids for twelve of the fatty acids. High levels of C20 PUFAs, an atypical observation in Chlorophytes, were observed in all three populations comprising approximately 17% of total fatty acids. Furthermore a 14:2 PUFA , apparently rare in marine macrophytic Chlorophytes, was identified in significant quantities. Surprisingly, we did not find any of the conjugated tetraene containing fatty acids that we previously identified in the A. stellata populations studied from the Florida Keys.

The fatty acid composition of the conidia and mycelia of the fungus Aspergillus nidulans

1986 ◽  
Vol 32 (2) ◽  
pp. 179-181 ◽  
Author(s):  
Joseph L. Evans ◽  
Michael A. Moclock ◽  
Michael A. Gealt
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Stationary Phase ◽  
Aspergillus Nidulans ◽  
Acid Composition ◽  
Fold Increase ◽  
Exponential Phase ◽  
Total Fatty Acids ◽  
Total Disappearance

The total fatty acids were characterized from conidia, exponential phase, and stationary phase Aspergillus nidulans. Several quantitative and qualitative variations were observed. Most notable was the approximately 15-fold increase in linolenate observed during the 1st day of incubation and its subsequent total disappearance by day 4.

Fatty Acids of Channel Catfish (Ictalurus punctatus)

1972 ◽  
Vol 29 (1) ◽  
pp. 113-115 ◽  
Author(s):  
R. E. Worthington ◽  
T. S. Boggess Jr. ◽  
E. K. Heaton
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Ictalurus Punctatus ◽  
Channel Catfish ◽  
Saturated Fatty Acids ◽  
Processing Plant ◽  
Gas Liquid Chromatography ◽  
Total Fatty Acids

Representative samples of channel catfish (Ictalurus punctatus Rafinesque) were obtained from five processing plants and analyzed by gas–liquid chromatography for fatty acid composition of muscle tissues. Twenty-three fatty acids were measured. The saturated fatty acids comprised 26.5%, monoenes 48.1%, dienes 13.0%, trienes 3.1%, tetraenes 3.6%, pentaenes 1.3%, and hexaenes 1.9% of total fatty acids. Fatty acid composition varied significantly (P <.01) among fish obtained from the same plant, and in addition, significant variation associated with processing plant was observed for some fatty acids.

scholarly journals Protein, Amino Acid, Fatty Acid Composition, and in Vitro Digestibility of Bread Fortified with Oncorhynchus tschawytscha Powder

Nutrients ◽  
2018 ◽  
Vol 10 (12) ◽  
pp. 1923 ◽  
Author(s):  
Ajay Desai ◽  
Tang Beibeia ◽  
Margaret Brennan ◽  
Xinbo Guo ◽  
Xin-An Zeng ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Amino Acid ◽  
Acid Composition ◽  
Protein Digestibility ◽  
Wheat Bread ◽  
Protein Amino Acid ◽  
Total Fatty Acids

This study investigated protein, amino acid, fatty acid composition, in vitro starch and protein digestibility, and phenolic and antioxidant composition of bread fortified with salmon fish (Oncorhynchus tschawytscha) powder (SFP). The proximate composition in control and SFP breads ranged between (34.00 ± 0.55–31.42 ± 0.73%) moisture, (13.91 ± 0.19–20.04 ± 0.10%) protein, (3.86 ± 0.02–9.13 ± 0.02%) fat, (2.13 ± 0.02–2.42 ± 0.09%) ash, (80.10 ± 0.018–68.42 ± 0.11%) carbohydrate, and (410.8 ± 0.18–435.96 ± 0.36 kcal) energy. The essential amino acids of the control and SFP breads ranged between 261.75 ± 9.23 and 306.96 ± 6.76 mg/g protein, which satisfies the score recommended by FAO/WHO/UNU (2007). Protein digestibility of the products was assessed using an in vitro assay. The protein digestibility, comma, amino acid score, essential amino acid index, biological value, and nutritional index ranged between 79.96 ± 0.65–80.80 ± 0.99%, 0.15 ± 0.06–0.42 ± 0.06%, 62.51 ± 1.15–76.68 ± 1.40%, 56.44 ± 1.05–71.68 ± 1.10%, 8.69 ± 0.10–15.36 ± 0.21%, respectively. Control and SFP breads contained 60.31 ± 0.21–43.60 ± 0.35 g/100 g total fatty acids (saturated fatty acids) and 13.51 ± 0.10–17.00 ± 0.09 g/100 g total fatty acids (polyunsaturated fatty acids), and SFP breads fulfil the ω-6/ω-3 score recommended by food authority. There was a significant effect of SFP on bread-specific volume, crumb color, and textural properties. The in vitro starch digestibility results illustrate that the incorporation of SFP into wheat bread decreased the potential glycemic response of bread and increased the antioxidant capacity of bread. In conclusion, this nutrient-rich SFP bread has the potential to be a technological alternative for the food industry.

Lipid composition of the trophosome in the beard worm, Oligobrachia mashikoi (Pogonophora)

2006 ◽  
Vol 86 (2) ◽  
pp. 283-286 ◽  
Author(s):  
Masatoshi Mita ◽  
Mariko Deguchi ◽  
Yuichi Sasayama
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Acid Composition ◽  
Lipid Composition ◽  
Cholesterol Ester ◽  
Palmitoleic Acid ◽  
Total Fatty Acids

A large quantity of triglyceride was detected histochemically in nutrient-deposit cells of the trophosome in the beard worm (Oligobrachia mashikoi). The lipid extracted from the trophosome was composed of triglyceride, several kinds of phospholipid, free fatty acid, cholesterol, and cholesterol ester. The fatty acid of the triglyceride was comprised mainly of a monoenoic type, such as palmitoleic acid (16:1) and oleic acid (18:1), which accounted for 31% and 37% of the total fatty acids, respectively. In addition, in blood triglyceride, the fatty acid composition was almost the same.

Fatty acid composition of sow's colostrum, milk and body fat as determined by gas-liquid chromatography

1963 ◽  
Vol 30 (3) ◽  
pp. 339-343 ◽  
Author(s):  
J. M. deMan ◽  
J. P. Bowland
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Liquid Chromatography ◽  
Fatty Acid ◽  
Acid Composition ◽  
Milk Fat ◽  
Gas Liquid Chromatography ◽  
Nutrient Requirements ◽  
Total Fatty Acids ◽  
The Mean

SummaryAs determined by gas-liquid chromatography, the mean fatty acid composition (weight percentages of total fatty acids) of milk fat from sows fed a diet to meet U.S. N.R.C. nutrient requirements was: oleic, 35·3; palmitic, 30·3; linoleic, 13·0; palmitoleic, 9·9; stearic, 4·0; myristic, 3·3; linolenic, 2·5; unidentified 0·7 and 0·5, presumably n-odd chain and branched fatty acids; lauric, 0·3; and capric, 0·2. The corresponding fatty acid composition of colostrum fat was: oleic, 41·7; palmitic, 22·5; linoleic, 20·9; palmitoleic, 5·0; stearic, 5·7; myristic, 1·4; linolenic, 2·4; and unidentified acids, 0·3 and 0·1. Dietary fat increased fat levels in the milk and influenced fatty acid composition of the milk fat. Backfat resembled colostrum fat more than milk fat.

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