scholarly journals Mode of action of a bactericidal protein induced in the haemolymph of Sarcophaga peregrina (flesh-fly) larvae

1984 ◽  
Vol 222 (1) ◽  
pp. 119-124 ◽  
Author(s):  
M Okada ◽  
S Natori
Keyword(s):  
Escherichia Coli ◽  
Membrane Potential ◽  
Mode Of Action ◽  
Flesh Fly ◽  
Fly Larvae

The mode of action of a bactericidal protein (sarcotoxin I) purified from the haemolymph of Sarcophaga peregrina (flesh-fly) larvae was studied, focusing attention on its effect on the function of the membrane of Escherichia coli. Sarcotoxin I almost completely blocked the uptakes of tetraphenylphosphonium ion and proline, which are known to be driven by a membrane potential, indicating that this protein disrupts the membrane potential of bacteria.

scholarly journals The use of valinomycin, nigericin and trichlorocarbanilide in control of the protonmotive force in Escherichia coli cells

1983 ◽  
Vol 212 (1) ◽  
pp. 105-112 ◽  
Author(s):  
S Ahmed ◽  
I R Booth
Keyword(s):  
Escherichia Coli ◽  
Membrane Potential ◽  
Mode Of Action ◽  
Ph Gradient ◽  
Protonmotive Force ◽  
Modes Of Action ◽  
Escherichia Coli Cells ◽  
Low Concentrations ◽  
High K ◽  
Specific Control

Valinomycin, nigericin and trichlorocarbanilide were assessed for their ability to control the protonmotive force in Escherichia coli cells. Valinomycin, at high K+ concentrations, was found to decrease the membrane potential delta phi and indirectly to decrease the pH gradient delta pH. Nigericin was found to have two modes of action. At low concentrations (0.05-2 microM) it carried out K+/H+ exchange and decreased delta pH. At higher concentrations (50 microM) it carried out a K+-dependent transfer of H+, decreasing both delta phi and delta pH. In EDTA-treated cells only the latter mode of action was evident, whereas in a mutant sensitive to deoxycholate both types of effect were observed. Trichlorocarbanilide is proposed as an alternative to nigericin for the specific control of delta pH, and it can be used in cells not treated with EDTA.

scholarly journals Nukacin ISK-1, a Bacteriostatic Lantibiotic

2009 ◽  
Vol 53 (8) ◽  
pp. 3595-3598 ◽  
Author(s):  
Sikder M. Asaduzzaman ◽  
Jun-ichi Nagao ◽  
Hiroshi Iida ◽  
Takeshi Zendo ◽  
Jiro Nakayama ◽  
...  
Keyword(s):  
Cell Wall ◽  
Membrane Potential ◽  
Bacterial Strain ◽  
Mode Of Action ◽  
Membrane Depolarization ◽  
Wall Width ◽  
Cytoplasmic Content ◽  
Remarkable Reduction

ABSTRACT We determined the mode of action of nukacin ISK-1. It did not cause membrane potential dissipation or the efflux of ATP or K+ ions from the cells of a sensitive bacterial strain; however, it blocked the membrane depolarization activity of nisin. Nukacin ISK-1-treated cells had single arrangements of cells without the formation of a complete septum. A remarkable reduction in cell wall width was also observed, but cytoplasmic content was unaffected. We concluded that nukacin ISK-1 is bacteriostatic.

scholarly journals Electron Microscopic Studies in <i>Escherichia Coli</i> on Mode of Action of Sodium Benzoate and Potassium Sorbate

2019 ◽  
Vol 4 (4) ◽  
pp. 82
Author(s):  
Norma Angélica Santiesteban-López ◽  
Teresa Gladys Cerón-Carrillo ◽  
José Luis Carmona-Silva ◽  
Javier Castro-Rosas
Keyword(s):  
Escherichia Coli ◽  
Mode Of Action ◽  
Sodium Benzoate ◽  
Potassium Sorbate ◽  
Electron Microscopic ◽  
Microscopic Studies

Mode of action of gramicidin S on Escherichia coli membrane

1986 ◽  
Vol 860 (3) ◽  
pp. 608-619 ◽  
Author(s):  
Takashi Katsu ◽  
Hideki Kobayashi ◽  
Yuzaburo Fujita
Keyword(s):  
Escherichia Coli ◽  
Mode Of Action ◽  
Gramicidin S
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