Effect of mutation, electric membrane potential, and metabolic inhibitors on the accessibility of nucleic acids to ethidium bromide in Escherichia coli cells

Biochemistry ◽  
1984 ◽  
Vol 23 (1) ◽  
pp. 166-176 ◽  
Author(s):  
Bernard Lambert ◽  
Jean Bernard Le Pecq
Keyword(s):  
Escherichia Coli ◽  
Membrane Potential ◽  
Nucleic Acids ◽  
Ethidium Bromide ◽  
Metabolic Inhibitors ◽  
Escherichia Coli Cells

scholarly journals The use of valinomycin, nigericin and trichlorocarbanilide in control of the protonmotive force in Escherichia coli cells

1983 ◽  
Vol 212 (1) ◽  
pp. 105-112 ◽  
Author(s):  
S Ahmed ◽  
I R Booth
Keyword(s):  
Escherichia Coli ◽  
Membrane Potential ◽  
Mode Of Action ◽  
Ph Gradient ◽  
Protonmotive Force ◽  
Modes Of Action ◽  
Escherichia Coli Cells ◽  
Low Concentrations ◽  
High K ◽  
Specific Control

Valinomycin, nigericin and trichlorocarbanilide were assessed for their ability to control the protonmotive force in Escherichia coli cells. Valinomycin, at high K+ concentrations, was found to decrease the membrane potential delta phi and indirectly to decrease the pH gradient delta pH. Nigericin was found to have two modes of action. At low concentrations (0.05-2 microM) it carried out K+/H+ exchange and decreased delta pH. At higher concentrations (50 microM) it carried out a K+-dependent transfer of H+, decreasing both delta phi and delta pH. In EDTA-treated cells only the latter mode of action was evident, whereas in a mutant sensitive to deoxycholate both types of effect were observed. Trichlorocarbanilide is proposed as an alternative to nigericin for the specific control of delta pH, and it can be used in cells not treated with EDTA.

Probes of membrane potential in Escherichia coli cells

FEBS Letters ◽  
1981 ◽  
Vol 125 (2) ◽  
pp. 197-200 ◽  
Author(s):  
Alexandre Ghazi ◽  
Emanuel Schechter ◽  
Lucienne Letellier ◽  
Bernard Labedan
Keyword(s):  
Escherichia Coli ◽  
Membrane Potential ◽  
Escherichia Coli Cells

DETECTION OF GAMMA RADIATION EFFECT INDUCED BY COBELT-60 ON ESCHERICHIA COLI CELLS

2010 ◽  
Vol 13 (3) ◽  
pp. 129-133
Author(s):  
Ghusoon Ali Abdul Hasan Al-Sudany ◽  
◽  
Wasan Zuheir Majeed ◽  
Hind Jabbar Abdul Rhman Akram Al-Aubeidi ◽  
◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gamma Radiation ◽  
Radiation Effect ◽  
Escherichia Coli Cells
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