scholarly journals Hormonal regulation of two urea-cycle enzymes in cultured foetal hepatocytes

1983 ◽  
Vol 216 (2) ◽  
pp. 281-285 ◽  
Author(s):  
A Husson ◽  
M Bouazza ◽  
C Buquet ◽  
R Vaillant
Keyword(s):  
Enzyme Activities ◽  
Hormonal Regulation ◽  
Cultured Cells ◽  
Urea Cycle ◽  
Rat Hepatocytes ◽  
Isolated Cells ◽  
Primary Monolayer Culture ◽  
Primary Monolayer ◽  
Urea Cycle Enzymes ◽  
Stimulation Of

Foetal-rat hepatocytes were cultured in primary monolayer culture, and activity changes of argininosuccinate synthetase (ASS, EC 6.3.4.5) and argininosuccinase (ASL, EC 4.3.2.1) were followed under defined hormone conditions. In hormone-free medium, cultured cells maintained the enzyme activities at values equal to those of freshly isolated cells for at least 3 days. Continuous addition of dexamethasone produced the development of the two enzyme activities, but only after the first 20h of culture. Under these conditions, urea production by the foetal hepatocytes was concomitantly increased in the culture medium. Pretreatment with dexamethasone for 20h was sufficient to produce the development of ASL activity within the 2 following days. Introduced alone, glucagon induced an increase of ASL activity, but did not affect the ASS activity. The most powerful stimulation of ASS and ASL could be observed in cultured hepatocytes if glucagon and dexamethasone were added simultaneously or sequentially. These results indicated that the development of the receptor complex for the induction of urea-cycle enzymes appears early before birth and established that glucocorticoids amplify the glucagon stimulation of these enzyme activities during foetal life.

scholarly journals Exposure of cultured hepatocytes to cyclic AMP enhances the vasopressin-mediated stimulation of inositol phosphate production

1989 ◽  
Vol 257 (2) ◽  
pp. 455-460 ◽  
Author(s):  
R A Pittner ◽  
J N Fain
Keyword(s):  
Cyclic Amp ◽  
Inositol Phosphate ◽  
Rat Hepatocytes ◽  
Isolated Rat Hepatocytes ◽  
Phosphate Accumulation ◽  
Primary Monolayer Culture ◽  
Inositol Phosphate Production ◽  
Inositol Phosphate Accumulation ◽  
Primary Monolayer ◽  
Stimulation Of

Isolated rat hepatocytes in primary monolayer culture were maintained for 18-24 h in the presence of 10% (v/v) serum and [3H]inositol. Vasopressin (100 nM) stimulated the production of inositol mono-, bis- and tris-phosphates (IP1, IP2, and IP3). Prior exposure of hepatocytes to 8-bromo cyclic AMP (8Br-cAMP; 100 microM), but not 8-bromo cyclic GMP, enhanced the vasopressin-mediated stimulation of inositol phosphate accumulation, but had no significant effect on their formation in the absence of vasopressin. The effect of the cyclic AMP analogue was mimicked by glucagon (10 nM), and was seen whether cyclic AMP or glucagon was added 5 min or 12 h before the addition of vasopressin. An 8 h incubation with dexamethasone (100 nM) enhanced the accumulation of IP3, but not that of IP2 or IP1, in the presence of 8Br-cAMP and vasopressin. Cycloheximide or actinomycin D had little effect on the vasopressin stimulation of inositol phosphate accumulation, after an 8 h incubation in the presence or absence of 8Br-cAMP.

scholarly journals The effect of insulin-dextran complexes on the protein synthesis in the primary monolayer culture of adult rat hepatocytes.

1984 ◽  
Vol 144 (3) ◽  
pp. 245-256
Author(s):  
TSUGUHIKO NAKAI ◽  
YASUNORI KUTSUMI ◽  
YOSHIKAZU SAKAMOTO ◽  
KOJI OIDA ◽  
SUSUMU MIYABO ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Monolayer Culture ◽  
Rat Hepatocytes ◽  
Adult Rat ◽  
Primary Monolayer Culture ◽  
Adult Rat Hepatocytes ◽  
Primary Monolayer

scholarly journals Subzero nonfreezing storage of rat hepatocytes using UW solution and 1,4-butanediol. II- functional testing on rewarming and gene expression of urea cycle enzymes

2009 ◽  
Vol 8 (2) ◽  
pp. 129-133 ◽  
Author(s):  
Edgardo E. Guibert ◽  
Luciana L. Almada ◽  
Maria E. Mamprin ◽  
Cristina Bellarosa ◽  
Maria Dolores Pizarro ◽  
...  
Keyword(s):  
Gene Expression ◽  
Urea Cycle ◽  
Rat Hepatocytes ◽  
Functional Testing ◽  
Uw Solution ◽  
Urea Cycle Enzymes

Effect of growth hormone on polynucleotide phosphorylase activity in a primary monolayer culture of rat hepatocytes

1980 ◽  
Vol 90 (6) ◽  
pp. 1688-1690
Author(s):  
V. P. Fedotov ◽  
G. N. Pluzhnikova ◽  
�. B. Khoroshutina ◽  
�. S. Yanakopulu ◽  
L. E. Andrianova
Keyword(s):  
Growth Hormone ◽  
Monolayer Culture ◽  
Rat Hepatocytes ◽  
Polynucleotide Phosphorylase ◽  
Phosphorylase Activity ◽  
Primary Monolayer Culture ◽  
Primary Monolayer
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