Effect of growth hormone on polynucleotide phosphorylase activity in a primary monolayer culture of rat hepatocytes

1980 ◽  
Vol 90 (6) ◽  
pp. 1688-1690
Author(s):  
V. P. Fedotov ◽  
G. N. Pluzhnikova ◽  
�. B. Khoroshutina ◽  
�. S. Yanakopulu ◽  
L. E. Andrianova
Keyword(s):  
Growth Hormone ◽  
Monolayer Culture ◽  
Rat Hepatocytes ◽  
Polynucleotide Phosphorylase ◽  
Phosphorylase Activity ◽  
Primary Monolayer Culture ◽  
Primary Monolayer

scholarly journals The effect of insulin-dextran complexes on the protein synthesis in the primary monolayer culture of adult rat hepatocytes.

1984 ◽  
Vol 144 (3) ◽  
pp. 245-256
Author(s):  
TSUGUHIKO NAKAI ◽  
YASUNORI KUTSUMI ◽  
YOSHIKAZU SAKAMOTO ◽  
KOJI OIDA ◽  
SUSUMU MIYABO ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Monolayer Culture ◽  
Rat Hepatocytes ◽  
Adult Rat ◽  
Primary Monolayer Culture ◽  
Adult Rat Hepatocytes ◽  
Primary Monolayer

scholarly journals Regulation of Hepatic Triglyceride Lipase by Insulin in Rat Hepatocytes in Primary Monolayer Culture

1980 ◽  
Vol 7 (4) ◽  
pp. 817-824
Author(s):  
Yasunori KUTSUMI ◽  
Tsuguhiko NAKAI ◽  
Shiro YAMADA ◽  
Toshitaka TAMAI ◽  
Takeshi KOBAYASHI ◽  
...  
Keyword(s):  
Monolayer Culture ◽  
Rat Hepatocytes ◽  
Hepatic Triglyceride ◽  
Triglyceride Lipase ◽  
Primary Monolayer Culture ◽  
Primary Monolayer ◽  
Hepatic Triglyceride Lipase

Transmembrane potential of rat hepatocytes in primary monolayer culture

1981 ◽  
Vol 241 (5) ◽  
pp. C209-C214 ◽  
Author(s):  
R. Wondergem
Keyword(s):  
Transmembrane Potential ◽  
Monolayer Culture ◽  
Input Resistance ◽  
Rat Hepatocytes ◽  
Collagen Gels ◽  
Shunt Resistance ◽  
Transmembrane Potentials ◽  
Primary Monolayer Culture ◽  
Primary Monolayer ◽  
The Mean

Transmembrane potentials of rat hepatocytes in primary monolayer culture on collagen gels were measured with glass microelectrodes. Potentials for cells in culture for 23-30 h comprised two populations. The mean +/- SD for a population of stable low potentials was -9.7 +/- 2.0 mV (n = 93). This was compared with -23.6 +/- 9.4 mV (n = 42), the mean value for stable potentials that followed spontaneous increases in the low potentials, 0.5-2.0 min after the impalement. The estimated input resistance increased during these spontaneous hyperpolarizations. In some cells, after 48 h in culture, the transmembrane potential oscillated rhythmically, with an amplitude of 25 mV and a period of 7 min. Suffusing the cells with 120 mM potassium chloride decreased the potential and eliminated the oscillations. The stable high potentials were considered more accurate estimates of the hepatocyte transmembrane potential, based on comparison with values for intact liver. Low potentials may have resulted from current leaking through an electrode shunt resistance, followed by an increase in potential as the membrane "sealed" the shunt pathway. However, these events may also reflect cells capable of two stable transmembrane potentials.

Assay of cholesterol 7α-hydroxylase activity in rat hepatocytes in primary monolayer culture

1988 ◽  
Vol 171 (1) ◽  
pp. 158-165 ◽  
Author(s):  
Hans M.G. Princen ◽  
Piet Meijer ◽  
Jaap Kwekkeboom ◽  
Herman Jan M. Kempen
Keyword(s):  
Monolayer Culture ◽  
Rat Hepatocytes ◽  
Hydroxylase Activity ◽  
Primary Monolayer Culture ◽  
Primary Monolayer

scholarly journals PRIMARY MONOLAYER CULTURE OF ADULT RAT HEPATOCYTES IN A SERUM-FREE SYSTEM: METABOLIC AND MORPHOLOGICAL STUDIES

1981 ◽  
Vol 2 (5) ◽  
pp. 491-500
Author(s):  
SHIRO YAMADA ◽  
TSUGUHIKO NAKAI ◽  
YASUNORI KUTSUMI ◽  
RYOYU TAKEDA ◽  
KYUICHI KURAKANE ◽  
...  
Keyword(s):  
Monolayer Culture ◽  
Rat Hepatocytes ◽  
Free System ◽  
Serum Free ◽  
Adult Rat ◽  
Primary Monolayer Culture ◽  
Adult Rat Hepatocytes ◽  
Primary Monolayer
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