scholarly journals Measles-virus-persistent infection in BGM cells. Modification of the incorporation of [3H]arachidonic acid and [14C]stearic acid into lipids

1983 ◽  
Vol 214 (3) ◽  
pp. 665-670 ◽  
Author(s):  
P Anderton ◽  
T F Wild ◽  
G Zwingelstein
Keyword(s):  
Fatty Acids ◽  
Arachidonic Acid ◽  
Stearic Acid ◽  
Measles Virus ◽  
Total Phospholipid ◽  
Persistently Infected ◽  
Esterified Fatty Acids ◽  
Acid Incorporation ◽  
Significant Difference ◽  
Infected Cells

In BGM cells chronically infected with measles virus, although the composition of the phospholipids is unaltered, the fatty acid composition is modified. Uninfected, lytic and persistently infected cells were labelled with [3H]arachidonic acid and [14C]stearic acid and their metabolic fate analysed. No difference in the total incorporation was observed in the different systems. However, the [14C]stearic acid and [3H]arachidonic acid were incorporated up to 2-fold and 13-fold respectively greater into the neutral lipid of persistently infected compared with that of uninfected cells. Both radioactive fatty acids were specifically accumulated in the triacylglycerol and non-esterified fatty acids fractions. Lytically infected cells were similar to uninfected cells. Although there was no significant difference in the incorporation of radioactivity into the total phospholipid in either system, there was a large decrease in [3H]arachidonic acid incorporated into phosphatidylethanolamine and to a lesser extent phosphatidylcholine and phosphatidylinositol in persistently infected cells. [14C]Stearic acid incorporation was also reduced in phosphatidylcholine and phosphatidylethanolamine fractions of persistently infected cells.

Infective substructures of measles virus from acutely and persistently infected cells.

1979 ◽  
Vol 32 (1) ◽  
pp. 329-333 ◽  
Author(s):  
S Rozenblatt ◽  
T Koch ◽  
O Pinhasi ◽  
S Bratosin
Keyword(s):  
Measles Virus ◽  
Persistently Infected ◽  
Infected Cells

scholarly journals Clearance of Measles Virus from Persistently Infected Cells by Short Hairpin RNA

2009 ◽  
Vol 83 (18) ◽  
pp. 9423-9431 ◽  
Author(s):  
Michael Zinke ◽  
Sabine Kendl ◽  
Katrin Singethan ◽  
Markus Fehrholz ◽  
Dajana Reuter ◽  
...  
Keyword(s):  
Measles Virus ◽  
Fluorescent Protein ◽  
Lentiviral Vector ◽  
Test System ◽  
Short Hairpin Rna ◽  
Hairpin Rna ◽  
Persistently Infected ◽  
Infected Cells ◽  
Short Hairpin ◽  
Nt2 Cells

ABSTRACT Subacute sclerosing panencephalitis (SSPE) is a demyelinating central nervous system disease caused by a persistent measles virus (MV) infection of neurons and glial cells. There is still no specific therapy available, and in spite of an intact innate and adaptive immune response, SSPE leads inevitably to death. In order to select effective antiviral short interfering RNAs (siRNAs), we established a plasmid-based test system expressing the mRNA of DsRed2 fused with mRNA sequences of single viral genes, to which certain siRNAs were directed. siRNA sequences were expressed as short hairpin RNA (shRNA) from a lentiviral vector additionally expressing enhanced green fluorescent protein (EGFP) as an indicator. Evaluation by flow cytometry of the dual-color system (DsRed and EGFP) allowed us to find optimal shRNA sequences. Using the most active shRNA constructs, we transduced persistently infected human NT2 cells expressing virus-encoded HcRed (piNT2-HcRed) as an indicator of infection. shRNA against N, P, and L mRNAs of MV led to a reduction of the infection below detectable levels in a high percentage of transduced piNT2-HcRed cells within 1 week. The fraction of virus-negative cells in these cultures was constant over at least 3 weeks posttransduction in the presence of a fusion-inhibiting peptide (Z-Phe-Phe-Gly), preventing the cell fusion of potentially cured cells with persistently infected cells. Transduced piNT2 cells that lost HcRed did not fuse with underlying Vero/hSLAM cells, indicating that these cells do not express viral proteins any more and are “cured.” This demonstrates in tissue culture that NT2 cells persistently infected with MV can be cured by the transduction of lentiviral vectors mediating the long-lasting expression of anti-MV shRNA.

scholarly journals Volume and surface changes in vero cell and its nucleus after infection with measles virus: a stereological study

2011 ◽  
Vol 2 (1) ◽  
pp. 18
Author(s):  
Ali Noorafshan ◽  
Mohammad Motamedifar ◽  
Saied Karbalay-Doust
Keyword(s):  
Vero Cell ◽  
Post Infection ◽  
Measles Virus ◽  
Plaque Assay ◽  
Vero Cells ◽  
Uninfected Control ◽  
Mean Cell Volume ◽  
Significant Difference ◽  
Infected Cells ◽  
The Mean

Measles virus has no or indistinctive cytopathic effects (CPE) in cell couture system. Employment of some detecting methods like plaque assay or stereologic experiments, as a method of detecting of viral infection in the cells would be applicable. The aim of this study was investigating the early changes in quantitative parameters of measles virus infected Vero cells. Stereological methods using invariator, were applied for the first time to estimate cell and nucleus volume and cell surface of the infected Vero cell line with the measles virus.This method can be applied on other cultured cells.Vero cells grown in tissue culture plates for 48 hours at 36˚C were infected with 100TCID50 of AiK strain of measles virus. Volume and surface of the infected Vero cells were studied at 4, 9 and 25 hours post infection along with uninfected control cells. The mean cell volume and surface of the cells infected with measles virus, increased ~87% and ~50%, respectively, 4 hours post-infection, as compared with the uninfected control. The nuclei did not show any differences. The mean parameters of infected cells in other time intervals showed no significant difference comparing with the control cells. Although there are other specific methods, stereology may be used as an integrated protocol to detect cytophatic changes of the measles virus infected cells early in the permissive cell culture system.

Influence of Kynurenine, Neopterin, Noradrenaline and Pyridoxal-5-Phosphate on Cholesterol and Phospholipid Content and Phospholipid Biosynthesis in vitro

Pteridines ◽  
1993 ◽  
Vol 4 (3) ◽  
pp. 126-130 ◽  
Author(s):  
Vera Rudzite ◽  
Edite Jurika ◽  
Gilbert Reibnegger ◽  
Günter Weiss ◽  
Helmut Wachter ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Arachidonic Acid ◽  
Fatty Acid ◽  
Incubation Medium ◽  
Tissue Homogenate ◽  
Phospholipid Content ◽  
Phospholipid Biosynthesis ◽  
Acid Incorporation ◽  
Wet Weight ◽  
Fatty Acid Incorporation

Summary Incorporation of fatty acids into phospholipids has been investigated using samples of rat liver tissue homogenate, Krebs-Ringer-phosphate buffer (pH = 7.4) containing 0.3% albumin, fatty acid mixture and glyceroL The addition of L-kynurenine (4 nmol/g wet weight), D-eryhro-neopterin (5 and 30 pmol/g wet weight) and noradrenaline (4 nmol/g wet weight) to incubation medium induced an increase of saturated (palmitic acid) and decrease of poly-unsaturated (linoleic and arachidonic acid) fatty acids incorporation into phospholipids. The increase of saturated fatty acids incorporation into phospholipids was more pronounced after addition of neopterin and noradrenaline to the incubation medium while the decrease of linoleic and arachidonic acid synthesis was stimulated most with kynurenine. Moreover, kynurenine stimulated whereas neopterin depressed the oleic acid incorporation into phospholipids. These changes of fatty acid incorporation into phospholipids were followed by increase of cholesterol content in samples containing kynurenine, neopterin or noradrenalin. In contrast, phospholipid content decreased in samples containing kynurenine or noradrenalin, hut was not altered by supplementation of neopterin. Since the addition of kynurenine and neopterin to incubation medium for isolated fog heart resulted in an increased noradrenaline and decreased pyridoxal-5-phosphate content in the tissue, we also added pyridoxal-5-phosphate (4 nmol/g wet weight) to incubation medium for phospholipid biosynthesis. No change of the fatty acid incorporation into phospholipids as welI as the content of phospholipids and cholesterol in samples was observed.

scholarly journals Characterization of plasma unsaturated lysophosphatidylcholines in human and rat

1999 ◽  
Vol 345 (1) ◽  
pp. 61-67 ◽  
Author(s):  
Martine CROSET ◽  
Nicole BROSSARD ◽  
Anne POLETTE ◽  
Michel LAGARDE
Keyword(s):  
Fatty Acids ◽  
Arachidonic Acid ◽  
Rat Plasma ◽  
Arachidonic Acid Content ◽  
Tissue Uptake ◽  
Membrane Phospholipids ◽  
Esterified Fatty Acids ◽  
The Brain ◽  
Group Shift

Unsaturated lysophosphatidylcholines (lysoPtdCho) bound to albumin circulate in blood plasma and seem to be a novel transport system for carrying polyunsaturated fatty acids (PUFA) to tissues that are rich in these fatty acids, such as the brain. The potential of these lysoPtdCho as a significant source of PUFA for cells has been assessed by comparing their plasma concentration with that of unsaturated non-esterified fatty acids (NEFA) bound to albumin. In humans, the PUFA concentration was 25.9±3.1 nmol/ml for these lysoPtdCho, compared with 33.4±9.6 nmol/ml for NEFA; in rats the equivalent values are 14.2±0.6 and 13.1±1.1 nmol/ml respectively (means±S.E.M.). The lysoPtdCho arachidonic acid content was 2-fold (human) and 5-fold (rat) higher than that of NEFA. In human and rat plasma, unsaturated lysoPtdCho were associated mainly with albumin rather than lipoproteins. The rate and extent of the acyl group shift from the sn-2 to sn-1 position of these lysoPtdCho were studied by the incubation of 1-lyso,2-[14C]C18:2n-6-glycerophosphocholine (GPC) with plasma. The rapid isomerization of this lipid occurred at pH 7 (20% isomerization within 2 min) and was not prevented by its association with albumin. The position of the acyl group in the lysoPtdCho circulating in plasma was studied by collecting blood directly in organic solvents containing 1-lyso,2-[14C]C18:2n-6-GPC as a marker of isomerization that occurred during sampling and analysis. Approx. 50% of the PUFA was located at the sn-2 position, demonstrating that substantial concentrations of 2-acyl-lysoPtdCho are present in plasma and are available for tissue uptake, where they can be reacylated at the sn-1 position to form membrane phospholipids.

Biosynthesis of measles virus hemagglutinin in persistently infected cells

1983 ◽  
Vol 75 (1-2) ◽  
pp. 87-101 ◽  
Author(s):  
W. J. Bellini ◽  
G. D. Silver ◽  
D. E. McFarlin
Keyword(s):  
Measles Virus ◽  
Persistently Infected ◽  
Infected Cells
Sign in / Sign up

Export Citation Format

Share Document