Biosynthesis of measles virus hemagglutinin in persistently infected cells

1983 ◽  
Vol 75 (1-2) ◽  
pp. 87-101 ◽  
Author(s):  
W. J. Bellini ◽  
G. D. Silver ◽  
D. E. McFarlin
Keyword(s):  
Measles Virus ◽  
Persistently Infected ◽  
Infected Cells

Infective substructures of measles virus from acutely and persistently infected cells.

1979 ◽  
Vol 32 (1) ◽  
pp. 329-333 ◽  
Author(s):  
S Rozenblatt ◽  
T Koch ◽  
O Pinhasi ◽  
S Bratosin
Keyword(s):  
Measles Virus ◽  
Persistently Infected ◽  
Infected Cells

scholarly journals Clearance of Measles Virus from Persistently Infected Cells by Short Hairpin RNA

2009 ◽  
Vol 83 (18) ◽  
pp. 9423-9431 ◽  
Author(s):  
Michael Zinke ◽  
Sabine Kendl ◽  
Katrin Singethan ◽  
Markus Fehrholz ◽  
Dajana Reuter ◽  
...  
Keyword(s):  
Measles Virus ◽  
Fluorescent Protein ◽  
Lentiviral Vector ◽  
Test System ◽  
Short Hairpin Rna ◽  
Hairpin Rna ◽  
Persistently Infected ◽  
Infected Cells ◽  
Short Hairpin ◽  
Nt2 Cells

ABSTRACT Subacute sclerosing panencephalitis (SSPE) is a demyelinating central nervous system disease caused by a persistent measles virus (MV) infection of neurons and glial cells. There is still no specific therapy available, and in spite of an intact innate and adaptive immune response, SSPE leads inevitably to death. In order to select effective antiviral short interfering RNAs (siRNAs), we established a plasmid-based test system expressing the mRNA of DsRed2 fused with mRNA sequences of single viral genes, to which certain siRNAs were directed. siRNA sequences were expressed as short hairpin RNA (shRNA) from a lentiviral vector additionally expressing enhanced green fluorescent protein (EGFP) as an indicator. Evaluation by flow cytometry of the dual-color system (DsRed and EGFP) allowed us to find optimal shRNA sequences. Using the most active shRNA constructs, we transduced persistently infected human NT2 cells expressing virus-encoded HcRed (piNT2-HcRed) as an indicator of infection. shRNA against N, P, and L mRNAs of MV led to a reduction of the infection below detectable levels in a high percentage of transduced piNT2-HcRed cells within 1 week. The fraction of virus-negative cells in these cultures was constant over at least 3 weeks posttransduction in the presence of a fusion-inhibiting peptide (Z-Phe-Phe-Gly), preventing the cell fusion of potentially cured cells with persistently infected cells. Transduced piNT2 cells that lost HcRed did not fuse with underlying Vero/hSLAM cells, indicating that these cells do not express viral proteins any more and are “cured.” This demonstrates in tissue culture that NT2 cells persistently infected with MV can be cured by the transduction of lentiviral vectors mediating the long-lasting expression of anti-MV shRNA.

scholarly journals Measles-virus-persistent infection in BGM cells. Modification of the incorporation of [3H]arachidonic acid and [14C]stearic acid into lipids

1983 ◽  
Vol 214 (3) ◽  
pp. 665-670 ◽  
Author(s):  
P Anderton ◽  
T F Wild ◽  
G Zwingelstein
Keyword(s):  
Fatty Acids ◽  
Arachidonic Acid ◽  
Stearic Acid ◽  
Measles Virus ◽  
Total Phospholipid ◽  
Persistently Infected ◽  
Esterified Fatty Acids ◽  
Acid Incorporation ◽  
Significant Difference ◽  
Infected Cells

In BGM cells chronically infected with measles virus, although the composition of the phospholipids is unaltered, the fatty acid composition is modified. Uninfected, lytic and persistently infected cells were labelled with [3H]arachidonic acid and [14C]stearic acid and their metabolic fate analysed. No difference in the total incorporation was observed in the different systems. However, the [14C]stearic acid and [3H]arachidonic acid were incorporated up to 2-fold and 13-fold respectively greater into the neutral lipid of persistently infected compared with that of uninfected cells. Both radioactive fatty acids were specifically accumulated in the triacylglycerol and non-esterified fatty acids fractions. Lytically infected cells were similar to uninfected cells. Although there was no significant difference in the incorporation of radioactivity into the total phospholipid in either system, there was a large decrease in [3H]arachidonic acid incorporated into phosphatidylethanolamine and to a lesser extent phosphatidylcholine and phosphatidylinositol in persistently infected cells. [14C]Stearic acid incorporation was also reduced in phosphatidylcholine and phosphatidylethanolamine fractions of persistently infected cells.

scholarly journals Characterization of Measles Virus-Specific Proteins Synthesized In Vivo and In Vitro from Acutely and Persistently Infected Cells

1979 ◽  
Vol 29 (3) ◽  
pp. 1099-1106 ◽  
Author(s):  
Shmuel Rozenblatt ◽  
Marian Gorecki ◽  
Helen Shure ◽  
Carol L. Prives
Keyword(s):  
Measles Virus ◽  
Persistently Infected ◽  
Specific Proteins ◽  
Infected Cells
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