scholarly journals Isodityrosine, a new cross-linking amino acid from plant cell-wall glycoprotein

1982 ◽  
Vol 204 (2) ◽  
pp. 449-455 ◽  
Author(s):  
S C Fry
Keyword(s):  
Cell Wall ◽  
Amino Acid ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Higher Plants ◽  
Diphenyl Ether ◽  
Covalent Binding ◽  
Sodium Dodecyl ◽  
Ether Bridge

1. Cell-wall hydrolysates from calli of all higher plants tested contained a new phenolic amino acid for which the trivial name isodityrosine is proposed. Isodityrosine was shown to be an oxidatively coupled dimer of tyrosine with the two tyrosine units linked by a diphenyl ether bridge. 2. The amount of isodityrosine in sodium dodecyl sulphate-insoluble cell-wall preparations was proportional to the amount of hydroxyproline. 3. Acidified chlorite split the diphenyl ether bridge of isodityrosine, and concomitantly solubilized the cell-wall glycoprotein. 4. Dithiothreitol inhibited isodityrosine synthesis in vivo, and suppressed in parallel the covalent binding of newly synthesized protein in the cell wall. 5. It is suggested that isodityrosine is an inter-polypeptide cross-link responsible for the insolubility of plant cell-wall glycoprotein.

scholarly journals Di-isodityrosine, a novel tetrametric derivative of tyrosine in plant cell wall proteins: a new potential cross-link

1996 ◽  
Vol 315 (1) ◽  
pp. 323-327 ◽  
Author(s):  
Jeffrey D. BRADY ◽  
Ian H. SADLER ◽  
Stephen C. FRY
Keyword(s):  
Cell Culture ◽  
Cell Wall ◽  
Amino Acid ◽  
Plant Cell ◽  
Cell Walls ◽  
Plant Cell Wall ◽  
Cell Wall Proteins ◽  
Cross Link ◽  
Nmr Spectrometry

A novel amino acid, di-isodityrosine, has been isolated from hydrolysates of cell walls of tomato cell culture. Analysis by UV spectrometry, partial derivatization with 2,4-dinitrofluorobenzene and mass and NMR spectrometry show that the compound is composed to two molecules of isodityrosine, joined by a biphenyl linkage. The possible reactions involved in the formation of this molecule in vivo are discussed, as is the possibility that it could form an interpolypeptide linkage between cell wall proteins such as extensin, and hence aid in the insolubilization of the protein in the wall.

In vitro and in vivo polysaccharides assembly: ultrastructural and cytochemical study of growing plant cell wall components.

1978 ◽  
Vol 36 (2) ◽  
pp. 434-435
Author(s):  
D. Reis ◽  
B. Vian ◽  
J. C. Roland
Keyword(s):  
Cell Wall ◽  
Self Assembly ◽  
Plant Cell Wall ◽  
Higher Plants ◽  
Three Dimensional ◽  
Cytochemical Study ◽  
Wall Components

Wall morphogenesis in higher plants is a problem still open to controversy. Until now the possibility of a transmembrane control and the involvement of microtubules were mostly envisaged. Self-assembly processes have been observed in the case of walls of Chlamydomonas and bacteria. Spontaneous gelling interactions between xanthan and galactomannan from Ceratonia have been analyzed very recently. The present work provides indications that some processes of spontaneous aggregation could occur in higher plants during the formation and expansion of cell wall.Observations were performed on hypocotyl of mung bean (Phaseolus aureus) for which growth characteristics and wall composition have been previously defined.In situ, the walls of actively growing cells (primary walls) show an ordered three-dimensional organization (fig. 1). The wall is typically polylamellate with multifibrillar layers alternately transverse and longitudinal. Between these layers intermediate strata exist in which the orientation of microfibrils progressively rotates. Thus a progressive change in the morphogenetic activity occurs.

scholarly journals Oxidative scission of plant cell wall polysaccharides by ascorbate-induced hydroxyl radicals

1998 ◽  
Vol 332 (2) ◽  
pp. 507-515 ◽  
Author(s):  
Stephen C. FRY
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Hydroxyl Radicals ◽  
Plant Cell Wall ◽  
Oh Radicals ◽  
Radical Scavengers ◽  
Cell Wall Polysaccharides ◽  
Ph Optimum ◽  
Living Plant

Scission of plant cell wall polysaccharides in vivo has generally been assumed to be enzymic. However, in the presence of l-ascorbate, such polysaccharides are shown to undergo non-enzymic scission under physiologically relevant conditions. Scission of xyloglucan by 1 mM ascorbate had a pH optimum of 4.5, and the maximum scission rate was reached after a 10–25-min delay. Catalase prevented the scission, whereas added H2O2 (0.1–10 mM) increased the scission rate and shortened the delay. Ascorbate caused detectable xyloglucan scission above approx. 5 µM. Dehydroascorbate was much less effective. Added Cu2+ (> 0.3 µM) also increased the rate of ascorbate-induced scission; EDTA was inhibitory. The rate of scission in the absence of added metals appeared to be attributable to the traces of Cu (2.8 mg·kg-1) present in the xyloglucan. Ascorbate-induced scission of xyloglucan was inhibited by radical scavengers; their effectiveness was proportional to their rate constants for reaction with hydroxyl radicals (•OH). It is proposed that ascorbate non-enzymically reduces O2 to H2O2, and Cu2+ to Cu+, and that H2O2 and Cu+ react to form •OH, which causes oxidative scission of polysaccharide chains. Evidence is reviewed to suggest that, in the wall of a living plant cell, Cu+ and H2O2 are formed by reactions involving ascorbate and its products, dehydroascorbate and oxalate. Systems may thus be in place to produce apoplastic •OH radicals in vivo. Although •OH radicals are often regarded as detrimental, they are so short-lived that they could act as site-specific oxidants targeted to play a useful role in loosening the cell wall, e.g. during cell expansion, fruit ripening and organ abscission.

One, Two, Three: A Bioorthogonal Triple Labelling Strategy for Studying the Dynamics of Plant Cell Wall Formation In Vivo

2018 ◽  
Vol 130 (51) ◽  
pp. 16907-16913 ◽  
Author(s):  
Clemence Simon ◽  
Cedric Lion ◽  
Corentin Spriet ◽  
Fabien Baldacci‐Cresp ◽  
Simon Hawkins ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Cell Wall Formation ◽  
Wall Formation

A click chemistry strategy for visualization of plant cell wall lignification

2014 ◽  
Vol 50 (82) ◽  
pp. 12262-12265 ◽  
Author(s):  
Yuki Tobimatsu ◽  
Dorien Van de Wouwer ◽  
Eric Allen ◽  
Robert Kumpf ◽  
Bartel Vanholme ◽  
...  
Keyword(s):  
Cell Wall ◽  
Click Chemistry ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Chemical Reporter

Monolignol mimics bearing chemical reporter tags and bioorthogonal click chemistry were commissioned to visualize plant cell wall lignins in vivo.

scholarly journals Enzyme-assisted in vivo polymerisation of conjugated oligomer based conductors

2020 ◽  
Vol 8 (19) ◽  
pp. 4221-4227 ◽  
Author(s):  
Gwennaël Dufil ◽  
Daniela Parker ◽  
Jennifer Y. Gerasimov ◽  
Thuc-Quyen Nguyen ◽  
Magnus Berggren ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Plant Structure

The conjugated oligomer ETE-S is enzymatically polymerized in vitro, in the presence of peroxidase and H2O2. This polymerization route occurs also in the plant cell wall where ETE-S polymerizes and forms conductors along the plant structure.

One, Two, Three: A Bioorthogonal Triple Labelling Strategy for Studying the Dynamics of Plant Cell Wall Formation In Vivo

2018 ◽  
Vol 57 (51) ◽  
pp. 16665-16671 ◽  
Author(s):  
Clemence Simon ◽  
Cedric Lion ◽  
Corentin Spriet ◽  
Fabien Baldacci‐Cresp ◽  
Simon Hawkins ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Cell Wall Formation ◽  
Wall Formation
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