Bioenergetic differences between MCF-7 and T47D breast cancer cells and their regulation by oestradiol and tamoxifen

2014 ◽  
Vol 465 (1) ◽  
pp. 49-61 ◽  
Author(s):  
Brandie N. Radde ◽  
Margarita M. Ivanova ◽  
Huy Xuan Mai ◽  
Joshua K. Salabei ◽  
Bradford G. Hill ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Real Time ◽  
Oestrogen Receptor ◽  
Breast Cancer Cells ◽  
Flux Analysis ◽  
Intact Cells ◽  
Extracellular Flux ◽  
Oestrogen Receptor Α ◽  
Mcf 7

Oestrogen receptor α-expressing breast cancer cells show differences in basal bioenergetics profiles and bioenergetics responses to serum depletion, oestradiol and tamoxifen as measured in real time by extracellular flux analysis in intact cells.

Xenoestrogens and the induction of proliferative effects in breast cancer cells via direct activation of oestrogen receptor α

2004 ◽  
Vol 21 (2) ◽  
pp. 134-144 ◽  
Author(s):  
A. G. Recchia ◽  
A. Vivacqua ◽  
S. Gabriele ◽  
A. Carpino ◽  
G. Fasanella ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Oestrogen Receptor ◽  
Breast Cancer Cells ◽  
Direct Activation ◽  
Oestrogen Receptor Α

scholarly journals The role of vulpinic acid as a natural compound in the regulation of breast cancer-associated miRNAs

2021 ◽  
Vol 54 (1) ◽  
Author(s):  
Demet Cansaran-Duman ◽  
Sevcan Yangın ◽  
Betül Çolak
Keyword(s):  
Breast Cancer ◽  
Cell Proliferation ◽  
Cancer Treatment ◽  
Cancer Cells ◽  
Real Time ◽  
Microarray Analysis ◽  
Mirna Expression ◽  
Breast Cancer Cells ◽  
Mcf 7

Abstract Background Breast cancer is the most frequently diagnosed cancer, and no effective treatment solution has yet been found. The number of studies based on the research of novel natural compounds in the treatment of breast cancer has been increasing in recent years. The anticancer properties of natural compounds are related to the regulation of microRNA (miRNA) expression. Therefore, changing the profile of miRNAs with the use of natural products is very important in cancer treatment. However, the role of vulpinic acid and related miRNAs in breast cancer progression remains unknown. Vulpinic acid, methyl (as2E)-2-(3-hydroxy-5-oxo-4-phenylfuran-2-ylidene)-2 phenylacetate, is a natural product extracted from the lichen species and shows an anticancer effect on different cancer cells. Methods This study examines the effects of vulpinic acid on the miRNA levels of breast cancer (MCF-7) cells and its relationship with cell proliferation and apoptosis levels. The antiproliferative effect of vulpinic acid was screened against MCF-7 breast cancer cells and MCF-12A breast epithelial cells using the xCELLigence real-time cell analysis system. We analyzed the altered miRNA expression profile in MCF-7 breast cancer cells versus MCF-12A cells following their response to vulpinic acid through microarray analysis. The microarray analysis results were confirmed through quantitative real-time PCR and bioinformatics analysis. Results The results of the miRNA array and bioinformatic analyses demonstrated that 12 miRNAs were specifically responsive to vulpinic acid in MCF-7 breast cancer cells. This is the first study to reveal that vulpinic acid inhibits the expression of 12 miRNAs and suppresses breast cancer cell proliferation. The study also revealed that vulpinic acid may downregulate the expression of 12 miRNAs by repressing the FOXO-3 gene. The miRNA targets were mainly found to play a role in the apoptosis, cell cycle and MAPK pathways. Moreover, Bcl-2, Bax, procaspase-3 and procaspase-9 protein levels were assessed by western blot analysis for validation of apoptosis at the protein level. Conclusion This study revealed the molecular mechanisms of vulpinic acid on breast cancer and showed that vulpinic acid regulates apoptosis signaling pathways by decreasing the expression of miRNAs. The miRNA expression patterns illuminate the underlying effect of vulpinic acid in breast cancer treatment. Graphical Abstract

scholarly journals Oestrogen deprivation induces chemokine production and immune cell recruitment in in vitro and in vivo models of oestrogen receptor-positive breast cancer

2021 ◽  
Vol 23 (1) ◽  
Author(s):  
Jody Hazlett ◽  
Virginia Niemi ◽  
Aziz Aiderus ◽  
Katelyn Powell ◽  
Lyn Wise ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Oestrogen Receptor ◽  
Breast Cancer Cells ◽  
Immune Cell ◽  
Human Peripheral Blood ◽  
Peripheral Blood Mononuclear ◽  
Oestrogen Deprivation ◽  
Mcf 7

Abstract Background Oestrogen receptor-positive (ER+) breast cancer is commonly treated using endocrine therapies such as aromatase inhibitors which block synthesis of oestradiol, but the influence of this therapy on the immune composition of breast tumours has not been fully explored. Previous findings suggest that tumour infiltrating lymphocytes and immune-related gene expression may be altered by treatment with aromatase inhibitors. However, whether these changes are a direct result of impacts on the host immune system or mediated through tumour cells is not known. We aimed to investigate the effect of oestrogen deprivation on the expression of chemokines and immune infiltration in vitro and in an ER+ immunocompetent mouse model. Methods RT-qPCR and a bead-based Bioplex system were used to investigate the expression of chemokines in MCF-7 breast cancer cells deprived of oestrogen. A migration assay and flow cytometry were used to measure the migration of human peripheral blood mononuclear cells (PBMCs) to MCF-7 cells grown without the main biologically active oestrogen, oestradiol. Using flow cytometry and immunohistochemistry, we examined the immune cell infiltrate into tumours created by injecting SSM3 ER+ breast cancer cells into wild-type, immunocompetent 129/SvEv mice. Results This study demonstrates that oestrogen deprivation increases breast cancer secretion of TNF, CCL5, IL-6, IL-8, and CCL22 and alters total human peripheral blood mononuclear cell migration in an in vitro assay. Oestrogen deprivation of breast cancer cells increases migration of CD4+ T cells and decreases migration of CD11c+ and CD14+ PBMC towards cancer cells. PBMC migration towards breast cancer cells can be reduced by treatment with the non-steroidal anti-inflammatory drugs, aspirin and celecoxib. Treatment with endocrine therapy using the aromatase inhibitor letrozole increases CD4+ T cell infiltration into ER+ breast cancer tumours in immune competent mice. Conclusions These results suggest that anti-oestrogen treatment of ER+ breast cancer cells can alter cytokine production and immune cells in the area surrounding the cancer cells. These findings may have implications for the combination and timing of anti-oestrogen therapies with other therapies.

Exploration of mechanisms of α-linolenic acid in reducing the growth of oestrogen receptor positive breast cancer cells (MCF-7)

2016 ◽  
Vol 24 ◽  
pp. 513-519 ◽  
Author(s):  
Julie K. Mason-Ennis ◽  
Lauren P. LeMay-Nedjelski ◽  
Ashleigh K.A. Wiggins ◽  
Lilian U. Thompson
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Linolenic Acid ◽  
Oestrogen Receptor ◽  
Breast Cancer Cells ◽  
Mcf 7 ◽  
Positive Breast Cancer

scholarly journals Induction of cell cycle arrest and apoptosis by copper complex Cu(SBCM)2 towards oestrogen-receptor positive MCF-7 breast cancer cells

RSC Advances ◽  
2019 ◽  
Vol 9 (32) ◽  
pp. 18359-18370 ◽  
Author(s):  
Jhi Biau Foo ◽  
Li Shan Ng ◽  
Ji Hui Lim ◽  
Pau Xien Tan ◽  
Yan Zhi Lor ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Cancer Cells ◽  
Topoisomerase I ◽  
Oestrogen Receptor ◽  
Breast Cancer Cells ◽  
Dna Topoisomerase ◽  
Cycle Arrest ◽  
Mcf 7

Cu(SBCM)2 binds to DNA topoisomerase I, which, in turn, induces cell cycle arrest and apoptosis in MCF-7 breast cancer cells, possibly via p53 signalling pathway.

scholarly journals Promoting E2F1-mediated apoptosis in oestrogen receptor-α-negative breast cancer cells

BMC Cancer ◽  
2014 ◽  
Vol 14 (1) ◽  
Author(s):  
María F Montenegro ◽  
María del Mar Collado-González ◽  
María Piedad Fernández-Pérez ◽  
Manel B Hammouda ◽  
Lana Tolordava ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Oestrogen Receptor ◽  
Breast Cancer Cells ◽  
Oestrogen Receptor Α

scholarly journals Co-regulated gene expression by oestrogen receptor α and liver receptor homolog-1 is a feature of the oestrogen response in breast cancer cells

2013 ◽  
Vol 41 (22) ◽  
pp. 10228-10240 ◽  
Author(s):  
Chun-Fui Lai ◽  
Koen D. Flach ◽  
Xanthippi Alexi ◽  
Stephen P. Fox ◽  
Silvia Ottaviani ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Cancer Cells ◽  
Oestrogen Receptor ◽  
Breast Cancer Cells ◽  
Oestrogen Receptor Α ◽  
Regulated Gene Expression
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