scholarly journals Collagen cross-linking compounds in human urine

1981 ◽  
Vol 197 (3) ◽  
pp. 759-762 ◽  
Author(s):  
Zeenat Gunja-Smith ◽  
Robert J. Boucek
Keyword(s):  
Human Urine ◽  
Chemical Characterization ◽  
Cross Linking ◽  
Collagen Cross Linking

We report the isolation and chemical characterization of collagen cross-linking compounds, 3-hydroxypyridinium and dihydroxylysinonorleucine, from human urine.

Chemical characterization of human urine albumin in proteinuria

1979 ◽  
Vol 35 (3) ◽  
pp. 316-318
Author(s):  
B. A. Lang ◽  
L. Morávek ◽  
B. Meloun
Keyword(s):  
Human Urine ◽  
Chemical Characterization ◽  
Urine Albumin

scholarly journals Isolation and chemical characterization of collagen in bovine pulmonary tissues

1975 ◽  
Vol 145 (2) ◽  
pp. 287-297 ◽  
Author(s):  
G Francis ◽  
J Thomas
Keyword(s):  
Soluble Fraction ◽  
Proteolytic Enzymes ◽  
Chemical Characterization ◽  
Insoluble Fraction ◽  
Cross Linking ◽  
Pancreatic Elastase ◽  
Insoluble Collagen ◽  
Interstitial Collagens ◽  
Carbohydrate Contents

1. The contents of the fibrous proteins collagen and elastin in the pleural and parenchymal regions of bovine lungs were determined. The collagen content was approx. 70% (g/100g of salt-extracted defatted powder) in each tissue, and the elastin content was 28% in pleura and 13.5% in parenchyma. 2. Purification of the insoluble collagen from the pleura and parenchyma of bovine lungs by various methods was attempted. The collagen fractions isolated after incubation of the pulmonary tissues with the proteolytic enzymes collagenase (“collagenase-soluble” fraction) or pancreatic elastase (“elastase-insoluble” fraction) each contained approx. 87% of the total collagen initially present. 3. Both collagen fractions were chemically analysed for their amino acid and carbohydrate contents and were found to be similar to those of the intact interstitial collagens isolated from skin, bone and tendon. 4. The contents of the two aldimine cross-linking compounds, dehydrohydroxylysinonorleucine and dehydrodihydroxylysinonorleucine, were determined in the bovine pulmonary collagen fractions, and were found to decrease with increasing age of the animals, and were similar to the values found in intact collagens from bone and tendon.

scholarly journals Physico-chemical Characterization of Solutions from Cooked Beans and Their Comparison with Human Urine: Fertilizer and Food?

2020 ◽  
pp. 9-27
Author(s):  
J. C. Fopoussi Tuebue ◽  
I. N. Tchinda ◽  
P. D. Djiotsa
Keyword(s):  
Electric Conductivity ◽  
Human Urine ◽  
Chemical Characterization ◽  
Mineral Salts ◽  
Two Fluids ◽  
Macro Elements ◽  
Cooking Process ◽  
Physico Chemical ◽  
Characterization Of Solutions

The present paper aims to highlight the chemical characteristics of solutions from cooked beans and to compare them with human urine. Solutions of cooked beans were produced by cooking variety of Phaseolus vulgaris L. known as “Meringue” without salts. After this stage, samples of those solutions and samples of the water used for the cooking process were collected for laboratory analysis. A solution from cooked beans is rich in mineral salts, particularly major macro elements (N and K) and minor macro elements (Ca, S, Mg). Concerning the third major macro element, notably the phosphorous, it is present in low amounts. The advantage of this fluid consists in its low amounts of sodium and chlorides, coupled to its low electric conductivity. This fluid has a pH of 6.31. It is made of about 90% of water. A deep parallelism can be established between the human urine and solutions from cooked beans. In fact, these two fluids are rich in nitrogen and potassium, and mainly made of water. But, in the detail, some particularities are present. Human urine has high amounts of sodium and chlorides, this coupled with a high electric conductivity. Concerning solutions from cooked beans, it has high amounts of calcium and magnesium, and a quite nil electric conductivity. The solutions from cooked beans do not require a dilution, but a ridging directly after its application in other to avoid the loose of sulfur and nitrogen through gas emanation. Moreover, the numerous nutrients contained in solutions from cooked beans can be gainfully recycled as soup after flavoring.

Olfactory and Chemical Characterization of Indoor Air-Towards a Psychophysical Model for Air Quality

1981 ◽  
Author(s):  
Birgitta Berglund ◽  
Ulf Berglund ◽  
Thomas Lindvall ◽  
Helene Nicander-Bredberg
Keyword(s):  
Air Quality ◽  
Indoor Air ◽  
Chemical Characterization

Rapid Isolation of High Molecular Weight Urokinase from Native Human Urine

1982 ◽  
Vol 47 (03) ◽  
pp. 197-202 ◽  
Author(s):  
Kurt Huber ◽  
Johannes Kirchheimer ◽  
Bernd R Binder
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Human Urine ◽  
Gel Filtration ◽  
Chain Structure ◽  
The Other ◽  
Single Chain ◽  
Apparent Homogeneity ◽  
Sequential Adsorption

SummaryUrokinase (UK) could be purified to apparent homogeneity starting from crude urine by sequential adsorption and elution of the enzyme to gelatine-Sepharose and agmatine-Sepharose followed by gel filtration on Sephadex G-150. The purified product exhibited characteristics of the high molecular weight urokinase (HMW-UK) but did contain two distinct entities, one of which exhibited a two chain structure as reported for the HMW-UK while the other one exhibited an apparent single chain structure. The purification described is rapid and simple and results in an enzyme with probably no major alterations. Yields are high enough to obtain purified enzymes for characterization of UK from individual donors.

PURIFICATION AND PARTIAL CHEMICAL CHARACTERIZATION OF SHEEP NEUROPHYSIN

1973 ◽  
Vol 74 (2) ◽  
pp. 226-236 ◽  
Author(s):  
Michel Chrétien ◽  
Claude Gilardeau
Keyword(s):  
Amino Acid ◽  
Polyacrylamide Gel Electrophoresis ◽  
Chemical Characterization ◽  
Terminal Amino Acid ◽  
Amino Acid Determination ◽  
Pituitary Glands ◽  
Terminal Amino ◽  
Partial Chemical ◽  
Acid Determination

ABSTRACT A protein isolated from ovine pituitary glands has been purified, and its homogeneity assessed by NH2- and COOH-terminal amino acid determination, ultracentrifugation studies, and polyacrylamide gel electrophoresis after carboxymethylation. Its chemical and immunochemical properties are closely similar to those of beef and pork neurophysins, less similar to those of human neurophysins. It contains no tryptophan (like other neurophysins) or histidine (like all except bovine neurophysin-I and human neurophysins). It has alanine at the NH2-terminus and valine at the COOH-terminus. Its amino acid composition is similar to, but not identical with those of porcine and bovine neurophysins.

Sorbinil prevents diabetes-induced increases in vascular permeability but does not alter collagen cross-linking

Diabetes ◽  
1985 ◽  
Vol 34 (7) ◽  
pp. 703-705 ◽  
Author(s):  
J. R. Williamson ◽  
K. Chang ◽  
E. Rowold ◽  
J. Marvel ◽  
M. Tomlinson ◽  
...  
Keyword(s):  
Vascular Permeability ◽  
Cross Linking ◽  
Collagen Cross Linking
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