scholarly journals Cell-free conversion of isopenicillin N into deacetoxycephalosporin C by Cephalosporium acremonium mutant M-0198

1981 ◽  
Vol 194 (2) ◽  
pp. 649-651 ◽  
Author(s):  
J E Baldwin ◽  
J W Keeping ◽  
P D Singh ◽  
C A Vallejo
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Cephalosporium Acremonium ◽  
Resistant Material ◽  
Free System ◽  
Cell Free System ◽  
Chromatography Analysis ◽  
A Cell ◽  
Isopenicillin N

In a cell-free system prepared by lysis of protoplasts of Cephalosporium acremonium mutant M-0198, isopenicillin N was converted into a penicillinase-resistant material that behaved like deacetoxycephalosporin C on high-pressure liquid chromatography analysis. This activity was found to be unstable to storage at -80 degrees C; 70-80% of the activity was lost after 1 day.

scholarly journals Conversion of isopenicillin N into penicillin N in cell-free extracts of Cephalosporium acremonium

1981 ◽  
Vol 194 (2) ◽  
pp. 645-647 ◽  
Author(s):  
G S Jayatilake ◽  
J A Huddleston ◽  
E P Abraham
Keyword(s):  
Cephalosporium Acremonium ◽  
Free System ◽  
Cell Free System ◽  
A Cell ◽  
Osmotic Lysis ◽  
Isopenicillin N

In a cell-free system prepared by osmotic lysis of protoplasts of Cephalosporium acremonium, isopenicillin N is converted into penicillin N. The epimerase activity of the system is labile.

scholarly journals Incorporation of 3H from δ-(l-α-amino (4,5-3H)adipyl)-l-cysteinyl-d-(4,4-3H)valine into isopenicillin N

1979 ◽  
Vol 184 (2) ◽  
pp. 421-426 ◽  
Author(s):  
J O'Sullivan ◽  
R C Bleaney ◽  
J A Huddleston ◽  
E P Abraham
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Adipic Acid ◽  
Experimental Error ◽  
Acid Oxidase ◽  
Cephalosporium Acremonium ◽  
Amino Acid Oxidase ◽  
Free System ◽  
A Cell ◽  
Isopenicillin N

1. delta-(L-alpha-Amino[4,5-3H]adipyl)-L-cysteinyl-D-[4,4-3H]valine has been synthesized from its constituent amino acids, the L-alpha-amino[4,5-3H]adipic acid being obtained by reduction with 3H2 of methyl 5-acetamido-5,5-diethoxycarbonylpent-2-enoate and subsequent decarboxylation and hydrolysis. 2. In a cell-free system prepared by lysis of protoplasts of Cephalosporium acremonium 3H was incorporated from the doubly labelled tripeptide into a compound that behaved like penicillin N or isopenicillin N. The relative specific radioactivities of the alpha-aminoadipyl and penicillamine moieties of the penicillin were the same (within experimental error) as those of the alpha-aminoadipic acid and valine residues respectively of the tripeptide. 3. The behaviour of the labelled alpha-aminoadipic acid from the penicillin to the L-amino acid oxidase of Crotalus adamanteus venom showed that it was mainly L-alpha-aminoadipic acid. 4. The results are consistent with the hypothesis that the carbon skeleton of the LLD-tripeptide is incorporated intact into the penicillin molecule and that the first product is isopenicillin N.

Incidence of Patulin in Apple, Pear, and Mixed Fruit Products Marketed in New South Wales

1992 ◽  
Vol 55 (10) ◽  
pp. 796-802 ◽  
Author(s):  
KATARINA BURDA
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
New South ◽  
New South Wales ◽  
Detection Limits ◽  
Chromatography Analysis ◽  
South Wales ◽  
Juice Concentrate

Three hundred twenty-eight (328) apple, pear, and mixed fruit products including juices, sauces, purees, jellies, diced apples, and apple pulps from 38 Australian producers were analyzed for patulin using the technique of high pressure liquid chromatography analysis. Patulin was detected in 75 of 258 juice and juice concentrate samples ranging from 5 to 50 μg/L and in 73 samples ranging from 51 to 1130 μg/L. Of 70 samples other than juices, patulin was detected in 18 samples at levels below 50 μg/kg of patulin. The detection limits for patulin in juice and other products were 5 μg/L and 5 μg/kg, respectively.

scholarly journals Polyamine concentrations in bone marrow aspirates of children with leukemia and other malignancies

Blood ◽  
1976 ◽  
Vol 47 (4) ◽  
pp. 695-701
Author(s):  
O Rennert ◽  
T Miale ◽  
J Shukla ◽  
D Lawson ◽  
J Frias
Keyword(s):  
Bone Marrow ◽  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Marked Elevation ◽  
Chromatography Analysis ◽  
Polyamine Analysis

High-pressure liquid chromatography analysis of polyamines in bone narrow from leukemic and nonleukemic subjects demonstrated increased concentrations of putrescine, spermidine, and spermine associated with increased cellularity. The most striking abnormality was the marked elevation of putrescine. Bone marrow polyamine analysis may be an adjunct for evaluation of leukemia patients.

scholarly journals Polyamine concentrations in bone marrow aspirates of children with leukemia and other malignancies

Blood ◽  
1976 ◽  
Vol 47 (4) ◽  
pp. 695-701 ◽  
Author(s):  
O Rennert ◽  
T Miale ◽  
J Shukla ◽  
D Lawson ◽  
J Frias
Keyword(s):  
Bone Marrow ◽  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Marked Elevation ◽  
Chromatography Analysis ◽  
Polyamine Analysis

Abstract High-pressure liquid chromatography analysis of polyamines in bone narrow from leukemic and nonleukemic subjects demonstrated increased concentrations of putrescine, spermidine, and spermine associated with increased cellularity. The most striking abnormality was the marked elevation of putrescine. Bone marrow polyamine analysis may be an adjunct for evaluation of leukemia patients.

Sign in / Sign up

Export Citation Format

Share Document