scholarly journals Conversion of isopenicillin N into penicillin N in cell-free extracts of Cephalosporium acremonium

1981 ◽  
Vol 194 (2) ◽  
pp. 645-647 ◽  
Author(s):  
G S Jayatilake ◽  
J A Huddleston ◽  
E P Abraham
Keyword(s):  
Cephalosporium Acremonium ◽  
Free System ◽  
Cell Free System ◽  
A Cell ◽  
Osmotic Lysis ◽  
Isopenicillin N

In a cell-free system prepared by osmotic lysis of protoplasts of Cephalosporium acremonium, isopenicillin N is converted into penicillin N. The epimerase activity of the system is labile.

scholarly journals Cell-free conversion of isopenicillin N into deacetoxycephalosporin C by Cephalosporium acremonium mutant M-0198

1981 ◽  
Vol 194 (2) ◽  
pp. 649-651 ◽  
Author(s):  
J E Baldwin ◽  
J W Keeping ◽  
P D Singh ◽  
C A Vallejo
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Cephalosporium Acremonium ◽  
Resistant Material ◽  
Free System ◽  
Cell Free System ◽  
Chromatography Analysis ◽  
A Cell ◽  
Isopenicillin N

In a cell-free system prepared by lysis of protoplasts of Cephalosporium acremonium mutant M-0198, isopenicillin N was converted into a penicillinase-resistant material that behaved like deacetoxycephalosporin C on high-pressure liquid chromatography analysis. This activity was found to be unstable to storage at -80 degrees C; 70-80% of the activity was lost after 1 day.

scholarly journals Incorporation of 3H from δ-(l-α-amino (4,5-3H)adipyl)-l-cysteinyl-d-(4,4-3H)valine into isopenicillin N

1979 ◽  
Vol 184 (2) ◽  
pp. 421-426 ◽  
Author(s):  
J O'Sullivan ◽  
R C Bleaney ◽  
J A Huddleston ◽  
E P Abraham
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Adipic Acid ◽  
Experimental Error ◽  
Acid Oxidase ◽  
Cephalosporium Acremonium ◽  
Amino Acid Oxidase ◽  
Free System ◽  
A Cell ◽  
Isopenicillin N

1. delta-(L-alpha-Amino[4,5-3H]adipyl)-L-cysteinyl-D-[4,4-3H]valine has been synthesized from its constituent amino acids, the L-alpha-amino[4,5-3H]adipic acid being obtained by reduction with 3H2 of methyl 5-acetamido-5,5-diethoxycarbonylpent-2-enoate and subsequent decarboxylation and hydrolysis. 2. In a cell-free system prepared by lysis of protoplasts of Cephalosporium acremonium 3H was incorporated from the doubly labelled tripeptide into a compound that behaved like penicillin N or isopenicillin N. The relative specific radioactivities of the alpha-aminoadipyl and penicillamine moieties of the penicillin were the same (within experimental error) as those of the alpha-aminoadipic acid and valine residues respectively of the tripeptide. 3. The behaviour of the labelled alpha-aminoadipic acid from the penicillin to the L-amino acid oxidase of Crotalus adamanteus venom showed that it was mainly L-alpha-aminoadipic acid. 4. The results are consistent with the hypothesis that the carbon skeleton of the LLD-tripeptide is incorporated intact into the penicillin molecule and that the first product is isopenicillin N.

scholarly journals Synthesis of δ-(α-aminoadipyl)cysteinylvaline and its role in penicillin biosynthesis

1976 ◽  
Vol 157 (3) ◽  
pp. 651-660 ◽  
Author(s):  
P A Fawcett ◽  
J J Usher ◽  
J A Huddleston ◽  
R C Bleaney ◽  
J J Nisbet ◽  
...  
Keyword(s):  
Penicillium Chrysogenum ◽  
Methyl Groups ◽  
Penicillin Biosynthesis ◽  
Cephalosporium Acremonium ◽  
Free System ◽  
Cell Free System ◽  
A Cell

1. The stereoisomers of delta-(alpha-aminoadipyl)-L-cysteinylvaline (LLD, LLL and DLD) were synthesized from valine labelled with 3H in its methyl groups or in the alpha position. L-Cysteinyl-D-[4,4′-3H]valine was also synthesized. 2. 3H was incorporated into a compound that behaved like penicillin N when the LLD tripeptide containing either a methyl- or an alpha-labelled valine residue was incubated with a cell-free system prepared by lysis of protoplasts of Cephalosporium acremonium. 3. Incorporation was not observed under these conditions from the labelled all-L- or DLD-tripeptide, from L-cysteinyl-D-[4,4′-3H]valine, or of Penicillium chrysogenum appeared to be the LLD isomer, like that from C. acremonium. 5. These findings are discussed in relation to penicillin biosynthesis.

scholarly journals Stoichiometry of oxygen consumption in the biosynthesis of isopenicillin from a tripeptide

1982 ◽  
Vol 203 (3) ◽  
pp. 791-793 ◽  
Author(s):  
R L White ◽  
E M M John ◽  
J E Baldwin ◽  
E P Abraham
Keyword(s):  
Oxygen Consumption ◽  
Oxygen Electrode ◽  
Stoichiometric Ratio ◽  
O2 Consumption ◽  
Free System ◽  
Cell Free System ◽  
A Cell ◽  
Isopenicillin N

The biosynthesis of isopenicillin N from delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine in a cell-free system has been correlated wih O2 consumption by two methods, involving the use of an oxygen-electrode and an n.m.r. spectrometer respectively. The results are consistent with a 1 : 1 stoichiometric ratio for the dioxygen consumed to the isopenicillin N formed.

scholarly journals Effect of detergents on sterol synthesis in a cell-free system of yeast

1982 ◽  
Vol 23 (6) ◽  
pp. 803-810
Author(s):  
S Hata ◽  
T Nishino ◽  
N Ariga ◽  
H Katsuki
Keyword(s):  
Sterol Synthesis ◽  
Free System ◽  
Cell Free System ◽  
A Cell

scholarly journals Reconstitution of Endosomal Proteolysis in a Cell-free System

1989 ◽  
Vol 264 (10) ◽  
pp. 5392-5399
Author(s):  
L S Mayorga ◽  
R Diaz ◽  
P D Stahl
Keyword(s):  
Free System ◽  
Cell Free System ◽  
A Cell
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