scholarly journals A species difference in nucleoside phosphorylase activity and inosine-stimulated insulin secretion in isolated islets of Langerhans

1977 ◽  
Vol 168 (3) ◽  
pp. 591-593 ◽  
Author(s):  
I L Campbell ◽  
K W Taylor
Keyword(s):  
Insulin Secretion ◽  
Insulin Release ◽  
Islets Of Langerhans ◽  
Species Differences ◽  
Species Difference ◽  
Isolated Islets ◽  
Phosphorylase Activity ◽  
Nucleoside Phosphorylase ◽  
Isolated Islets Of Langerhans

Inosine is a potent simulant of insulin release from rat but not from rabbit islets of Langerhans. Further investigation showed that nucleoside phosphorylase activity is exceptionally low in rabbit islets. The ability of inosine to promote insulin release seems to be related to islet nucleoside phosphorylase activity, which can display marked species differences.

Insulin release from isolated islets of Langerhans of the rat in organ culture

1973 ◽  
Vol 29 (7) ◽  
pp. 881-882 ◽  
Author(s):  
Brigitte Ziegler ◽  
R. Butter ◽  
H. -J. Hahn ◽  
R. Mehling ◽  
H. Fiedler
Keyword(s):  
Organ Culture ◽  
Insulin Release ◽  
Islets Of Langerhans ◽  
Isolated Islets ◽  
Isolated Islets Of Langerhans

scholarly journals Chromium-induced inhibition of insulin secretion from isolated Islets of Langerhans

Diabetologia ◽  
1980 ◽  
Vol 18 (3) ◽  
pp. 229-232 ◽  
Author(s):  
T. Ghafghazi ◽  
M. L. McDaniel ◽  
P. E. Lacy
Keyword(s):  
Insulin Secretion ◽  
Islets Of Langerhans ◽  
Isolated Islets ◽  
Isolated Islets Of Langerhans

scholarly journals Regulation of insulin secretion from islets of Langerhans rendered permeable by electric discharge

1982 ◽  
Vol 206 (1) ◽  
pp. 81-87 ◽  
Author(s):  
M. Adel Yaseen ◽  
Kevin C. Pedley ◽  
Simon L. Howell
Keyword(s):  
Insulin Secretion ◽  
B Cells ◽  
Insulin Release ◽  
Islets Of Langerhans ◽  
Electric Discharge ◽  
Significant Inhibition ◽  
Marker Enzyme ◽  
Ultrastructural Studies ◽  
Isolated Islets Of Langerhans ◽  
Vinblastine Sulphate

1. High-voltage electric discharge has been used to increase the permeability of B-cells of isolated islets of Langerhans to facilitate studies of the effects of normally impermeable substances on insulin secretion. 2. The application of an intense electric field increased the [14C]sucrose space of the islets from 37.8±3.1% to 86.2±5.2% of their total volume as assessed by 3H2O content. The cells remained permeable for at least 40min. 3. Ultrastructural studies showed no deleterious changes in the structure of the B-cells after discharge. 4. Insulin secretion from normal islets was unaffected by increasing the medium [Ca2+] from 10nm to 10μm. In the islets that had been rendered permeable by discharge, insulin secretion was significantly increased under these conditions, without any alteration in the release of lactate dehydrogenase, a cytoplasmic marker enzyme. 5. Studies of the dynamics of insulin release during perifusion showed that the response to increased (10μm) Ca2+ concentration was rapid and sustained over a period of at least 13min. 6. Secretion responses to Ca2+ in perifusion established that maximum release in permeabilized islets occurs at approx. 1μm-Ca2+ and half-maximum release occurs at approx. 0.6μm-Ca2+. 7. The study of the effect of agents that interfere with the microtubular microfilamentous system in B-cells using a perifusion system revealed that cytochalasin B caused a considerable increase, whereas vinblastine sulphate caused a significant inhibition, in insulin release in response to 1μm-Ca2+. 8. This technique should facilitate the study of the role of normally impermeable ions and metabolic intermediates in the regulation of insulin secretion.

Effect of Streptozotocin on Glucose-induced Insulin Secretion by Isolated Islets of Langerhans

Diabetes ◽  
1971 ◽  
Vol 20 (8) ◽  
pp. 513-518 ◽  
Author(s):  
P. Golden ◽  
L. Baird ◽  
W. J. Malaisse ◽  
F. Malaisse-Lagae ◽  
M. M. Walker
Keyword(s):  
Insulin Secretion ◽  
Islets Of Langerhans ◽  
Isolated Islets ◽  
Isolated Islets Of Langerhans
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