scholarly journals Biosynthesis of the first component of complement by human fibroblasts

1977 ◽  
Vol 167 (3) ◽  
pp. 647-660 ◽  
Author(s):  
Kenneth B. M. Reid ◽  
Ellen Solomon
Keyword(s):  
Human Serum ◽  
Cell Lines ◽  
De Novo ◽  
Culture Media ◽  
Human Fibroblasts ◽  
Sodium Dodecyl ◽  
Fibroblast Cell ◽  
Haemolytic Activity ◽  
Fibroblast Cell Lines

1. Haemolytic activity corresponding to that of the first component of complement (C1) was synthesized and secreted by all nine human fibroblast cell lines examined. No activity was found in the culture media of a variety of other human cell lines. 2. The component-C1 haemolytic activity secreted by the fibroblast lines behaved in an identical manner, in most respects, with that of the component-C1 haemolytic activity of human serum. The component-C1 haemolytic activity secreted by fibroblasts, however, was less susceptible to inhibition by rabbit fragment F(ab′)2 anti-(human subcomponent C1q) than was the component-C1 haemolytic activity of human serum. 3. Biosynthesis of fibroblast component-C1 haemolytic activity was inhibited by the presence of cycloheximide and regained on its removal. 4. Incorporation of radioactivity into proteins secreted by the fibroblasts and release of component-C1 haemolytic activity by the fibroblasts both increased in a linear manner until several days after the cultures had reached a state of confluent growth. 5. Radioactivity was incorporated into subcomponents C1q, C1r and C1s, as judged by the formation of specific immunoprecipitates and by absorption with immune aggregates. 6. The immunoprecipitates formed by using antisera against subcomponents C1r and C1s were run on polyacrylamide gels in sodium dodecyl sulphate, and this provided convincing physiochemical evidence for the biosynthesis of these subcomponents de novo. 7. The results obtained with immunoprecipitates formed by using anti-(subcomponent C1q) suggest that subcomponent C1q may be synthesized and secreted by fibroblast cell lines in vitro, in a form with a higher molecular weight than that of subcomponent C1q which is isolated by conventional techniques of protein fractionation from fresh serum.

Chromosome 7 suppresses indefinite division of nontumorigenic immortalized human fibroblast cell lines KMST-6 and SUSM-1

1993 ◽  
Vol 13 (10) ◽  
pp. 6036-6043
Author(s):  
T Ogata ◽  
D Ayusawa ◽  
M Namba ◽  
E Takahashi ◽  
M Oshimura ◽  
...  
Keyword(s):  
Cell Lines ◽  
Human Cell ◽  
Human Fibroblasts ◽  
Fibroblast Cell ◽  
Chromosome 7 ◽  
In Vitro Mutagenesis ◽  
Human Cell Lines ◽  
First Case ◽  
Normal Human

Using nontumorigenic immortalized human cell lines KMST-6 (KMST) and SUSM-1 (SUSM), we attempted to identify the chromosome that carries a putative senescence-related gene(s). These cell lines are the only ones that have been established independently from normal human diploid fibroblasts following in vitro mutagenesis. We first examined restriction fragment length polymorphisms on each chromosome of these immortalized cell lines and their parental cell lines and found specific chromosomal alterations common to these cell lines (a loss of heterozygosity in KMST and a deletion in SUSM) on the long arm of chromosome 7. In addition to these, we also found that introduction of chromosome 7 into these cell lines by means of microcell fusion resulted in the cessation of cell division, giving rise to cells resembling cells in senescence. Introduction of other chromosomes, such as chromosomes 1 and 11, on which losses of heterozygosity were also detected in one of the cell lines (KMST), to either KMST or SUSM cells or of chromosome 7 to several tumor-derived cell lines had no effect on their division potential. These results strongly suggest that a gene(s) affecting limited-division potential or senescence of normal human fibroblasts is located on chromosome 7, probably at the long arm of the chromosome, representing the first case in which a specific chromosome reverses the immortal phenotype of otherwise normal human cell lines.

In-vitro examination of the biocompatibility of fibroblast cell lines on alloplastic meshes and sterilized polyester mosquito mesh

Hernia ◽  
2016 ◽  
Vol 21 (3) ◽  
pp. 407-416 ◽  
Author(s):  
R. Wiessner ◽  
T. Kleber ◽  
N. Ekwelle ◽  
K. Ludwig ◽  
D.-U. Richter
Keyword(s):  
Cell Lines ◽  
Fibroblast Cell ◽  
In Vitro Examination ◽  
Fibroblast Cell Lines

8-Isocyanoamphilecta-11(20),15-diene, a new antimalarial isonitrile diterpene from the sponge Ciocalapata sp.

2009 ◽  
Vol 87 (5) ◽  
pp. 612-618 ◽  
Author(s):  
Chatchai Wattanapiromsakul ◽  
Naphatson Chanthathamrongsiri ◽  
Somchai Bussarawit ◽  
Supreeya Yuenyongsawad ◽  
Anuchit Plubrukarn ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Cell Lines ◽  
Nmr Spectra ◽  
Human Fibroblasts ◽  
Fibroblast Cell ◽  
The Other ◽  
Ergosterol Peroxide ◽  
Other Hand ◽  
Mcf 7 ◽  
Fibroblast Cell Lines

A new isonitrile diterpene of the amphilectane family, 8-isocyanoamphilecta-11(20),15-diene (4), was isolated from the sponge Ciocalapata sp., along with three known isonitriles, 8,15-diisocyano-11(20)-amphilectene (1), 7-isocyanoamphilecta-11(20),15-diene (2), and 8-isocyanoamphilecta-11(20),14-diene (3), and two steroidal peroxides, ergosterol peroxide (5) and 5α,9α-epidioxy-8α,14α-epoxy-(22E)-ergosta-6,22-dien-3β-ol (6). The structure of the new isonitrile was elucidated spectroscopically. In addition, anomalous multiplicities in the NMR spectra of some isolated isonitriles were observed and are reported here. The four isonitriles were strongly active against Plasmodium falciparum K1 with IC50 in a range of 0.09–1.07 μmol/L. Except for 1, which was cytotoxic against both MCF-7 and fibroblast cell lines, the other three diterpenes showed no significant cytotoxicity against either targeted cell lines. On the other hand, the steroidal peroxides 5 and 6, which were less active in the antimalarial bioassay (IC50 values of 6.28 and 7.13 µmol/L, respectively), were strongly cytotoxic against MCF-7 (IC50 values of 0.025 and 0.003 µmol/L, respectively), with very little toxicity against human fibroblasts.

scholarly journals Establishment and characterization of equine fibroblast cell lines transformed in vivo and in vitro by BPV-1: Model systems for equine sarcoids

Virology ◽  
2008 ◽  
Vol 373 (2) ◽  
pp. 352-361 ◽  
Author(s):  
Z.Q. Yuan ◽  
E.A. Gault ◽  
P. Gobeil ◽  
C. Nixon ◽  
M.S. Campo ◽  
...  
Keyword(s):  
Cell Lines ◽  
Fibroblast Cell ◽  
Model Systems ◽  
Fibroblast Cell Lines

Human gingival fibroblast cell lines in vitro.

1980 ◽  
Vol 15 (1) ◽  
pp. 53-70 ◽  
Author(s):  
George G. Rose ◽  
Toshihiko Yajima ◽  
Charles J. Mahan
Keyword(s):  
Cell Lines ◽  
Fibroblast Cell ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Cell Lines

Human gingival fibroblast cell lines in vitro.

1980 ◽  
Vol 15 (3) ◽  
pp. 267-287 ◽  
Author(s):  
Toshihiko Yajima ◽  
George G. Rose ◽  
Charles J. Mahan
Keyword(s):  
Cell Lines ◽  
Fibroblast Cell ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Cell Lines

Microscopic Assay for the Phagocytotic-Collagenolytic Performance (PCP Index) of Human Gingival Fibroblasts in vitro

1978 ◽  
Vol 57 (11-12) ◽  
pp. 1003-1015 ◽  
Author(s):  
George G. Rose ◽  
Toshihiko Yajima ◽  
Charles J. Mahan
Keyword(s):  
Tissue Culture ◽  
Thin Layer ◽  
Cell Lines ◽  
Fibroblast Cell ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Gingival Fibroblasts ◽  
Cover Slip ◽  
Fibroblast Cell Lines

Using 16 human gingival fibroblast cell lines from patients with periodontitis, Dilantin hyperplasia, and nonpathological gingiva, a microscopic assay was developed to quantitate the cells' ability to lyse collagen substrates. The method employs tissue culture chambers with one cover slip partially coated with a thin layer of undenatured fibrillar bovine codlagen. The assay measures the relative numbers and sizes of holes in the collagen within defined regions of the cover slips effected by the phagocytotic and collagenolytic performance (PCP) of the population of fibroblasts growing on the cover slip for 5 days. The effect on the PCP index by serum, heparin, prostaglandins, and endotoxin was evaluated.

scholarly journals Chromosome 7 suppresses indefinite division of nontumorigenic immortalized human fibroblast cell lines KMST-6 and SUSM-1.

1993 ◽  
Vol 13 (10) ◽  
pp. 6036-6043 ◽  
Author(s):  
T Ogata ◽  
D Ayusawa ◽  
M Namba ◽  
E Takahashi ◽  
M Oshimura ◽  
...  
Keyword(s):  
Cell Lines ◽  
Human Cell ◽  
Human Fibroblasts ◽  
Fibroblast Cell ◽  
Chromosome 7 ◽  
In Vitro Mutagenesis ◽  
Human Cell Lines ◽  
First Case ◽  
Normal Human

Using nontumorigenic immortalized human cell lines KMST-6 (KMST) and SUSM-1 (SUSM), we attempted to identify the chromosome that carries a putative senescence-related gene(s). These cell lines are the only ones that have been established independently from normal human diploid fibroblasts following in vitro mutagenesis. We first examined restriction fragment length polymorphisms on each chromosome of these immortalized cell lines and their parental cell lines and found specific chromosomal alterations common to these cell lines (a loss of heterozygosity in KMST and a deletion in SUSM) on the long arm of chromosome 7. In addition to these, we also found that introduction of chromosome 7 into these cell lines by means of microcell fusion resulted in the cessation of cell division, giving rise to cells resembling cells in senescence. Introduction of other chromosomes, such as chromosomes 1 and 11, on which losses of heterozygosity were also detected in one of the cell lines (KMST), to either KMST or SUSM cells or of chromosome 7 to several tumor-derived cell lines had no effect on their division potential. These results strongly suggest that a gene(s) affecting limited-division potential or senescence of normal human fibroblasts is located on chromosome 7, probably at the long arm of the chromosome, representing the first case in which a specific chromosome reverses the immortal phenotype of otherwise normal human cell lines.

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