Comparison of in vitro growth and tissue behavior of two different fibroblast cell lines embedded on an artificial scaffold

2001 ◽  
Vol 109 (5) ◽  
pp. 2416-2416
Author(s):  
Sanyukta Jaiswal ◽  
Ingo R. Titze ◽  
James A. Martin ◽  
Steven Gray
Keyword(s):  
Cell Lines ◽  
Fibroblast Cell ◽  
In Vitro Growth ◽  
Fibroblast Cell Lines

In-vitro examination of the biocompatibility of fibroblast cell lines on alloplastic meshes and sterilized polyester mosquito mesh

Hernia ◽  
2016 ◽  
Vol 21 (3) ◽  
pp. 407-416 ◽  
Author(s):  
R. Wiessner ◽  
T. Kleber ◽  
N. Ekwelle ◽  
K. Ludwig ◽  
D.-U. Richter
Keyword(s):  
Cell Lines ◽  
Fibroblast Cell ◽  
In Vitro Examination ◽  
Fibroblast Cell Lines

scholarly journals Establishment and characterization of equine fibroblast cell lines transformed in vivo and in vitro by BPV-1: Model systems for equine sarcoids

Virology ◽  
2008 ◽  
Vol 373 (2) ◽  
pp. 352-361 ◽  
Author(s):  
Z.Q. Yuan ◽  
E.A. Gault ◽  
P. Gobeil ◽  
C. Nixon ◽  
M.S. Campo ◽  
...  
Keyword(s):  
Cell Lines ◽  
Fibroblast Cell ◽  
Model Systems ◽  
Fibroblast Cell Lines

Human gingival fibroblast cell lines in vitro.

1980 ◽  
Vol 15 (1) ◽  
pp. 53-70 ◽  
Author(s):  
George G. Rose ◽  
Toshihiko Yajima ◽  
Charles J. Mahan
Keyword(s):  
Cell Lines ◽  
Fibroblast Cell ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Cell Lines

Human gingival fibroblast cell lines in vitro.

1980 ◽  
Vol 15 (3) ◽  
pp. 267-287 ◽  
Author(s):  
Toshihiko Yajima ◽  
George G. Rose ◽  
Charles J. Mahan
Keyword(s):  
Cell Lines ◽  
Fibroblast Cell ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Cell Lines

Microscopic Assay for the Phagocytotic-Collagenolytic Performance (PCP Index) of Human Gingival Fibroblasts in vitro

1978 ◽  
Vol 57 (11-12) ◽  
pp. 1003-1015 ◽  
Author(s):  
George G. Rose ◽  
Toshihiko Yajima ◽  
Charles J. Mahan
Keyword(s):  
Tissue Culture ◽  
Thin Layer ◽  
Cell Lines ◽  
Fibroblast Cell ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Gingival Fibroblasts ◽  
Cover Slip ◽  
Fibroblast Cell Lines

Using 16 human gingival fibroblast cell lines from patients with periodontitis, Dilantin hyperplasia, and nonpathological gingiva, a microscopic assay was developed to quantitate the cells' ability to lyse collagen substrates. The method employs tissue culture chambers with one cover slip partially coated with a thin layer of undenatured fibrillar bovine codlagen. The assay measures the relative numbers and sizes of holes in the collagen within defined regions of the cover slips effected by the phagocytotic and collagenolytic performance (PCP) of the population of fibroblasts growing on the cover slip for 5 days. The effect on the PCP index by serum, heparin, prostaglandins, and endotoxin was evaluated.

Fibroblast cell lines from young adult mice of long-lived mutant strains are resistant to multiple forms of stress

2005 ◽  
Vol 289 (1) ◽  
pp. E23-E29 ◽  
Author(s):  
Adam B. Salmon ◽  
Shin Murakami ◽  
Andrzej Bartke ◽  
John Kopchick ◽  
Kyoko Yasumura ◽  
...  
Keyword(s):  
Young Adult ◽  
Cell Lines ◽  
Uv Light ◽  
Dermal Fibroblast ◽  
Fibroblast Cell ◽  
Antioxidant Defenses ◽  
Adult Mice ◽  
Snell Dwarf ◽  
Fibroblast Cell Lines

Previous studies have shown that dermal fibroblast cell lines derived from young adult mice of the long-lived Snell dwarf mutant stock are resistant, in vitro, to the cytotoxic effects of H2O2, cadmium, UV light, paraquat, and heat. We show here that similar resistance profiles are seen in fibroblast cells derived from a related mutant, the Ames dwarf mouse, and that cells from growth hormone receptor-null mice are resistant to H2O2, paraquat, and UV but not to cadmium. Resistance to UV light, cadmium, and H2O2are similar in cells derived from 1-wk-old Snell dwarf or normal mice, and thus the resistance of cell lines derived from young adult donors reflects developmental processes, presumably hormone dependent, that take place in the first few months of life. The resistance of cells from Snell dwarf mice to these stresses does not reflect merely antioxidant defenses: dwarf-derived cells are also resistant to the DNA-alkylating agent methyl methanesulfonate. Furthermore, inhibitor studies show that fibroblast resistance to UV light is unaffected by the antioxidants ascorbic acid and N-acetyl-l-cysteine. These data suggest that postnatal exposure to altered levels of pituitary hormones leads to development of cellular resistance to oxidative and nonoxidative stressors, which are stable through many rounds of in vitro cell division and could contribute to the remarkable disease resistance of long-lived mutant mice.

scholarly journals Increased Antibacterial Properties of Indoline-Derived Phenolic Mannich Bases

2021 ◽  
Author(s):  
Tatu Rimpilainen ◽  
Alexandra Nunes ◽  
Rita Calado ◽  
Ana Fernandes ◽  
Joana Andrade ◽  
...  
Keyword(s):  
Cell Lines ◽  
Antibacterial Properties ◽  
Fibroblast Cell ◽  
Mannich Bases ◽  
Artemia Salina ◽  
Resistant Bacteria ◽  
Human Embryonic Lung ◽  
Fibroblast Cell Lines

<p>The search for antibacterial agents for the combat of nosocomial infections is a timely problem, as antibiotic-resistant bacteria continue to thrive. The effect of indoline substituents on the antibacterial properties of aminoalkylphenols was studied, leading to the development of a library of compounds with minimum inhibitory concentrations (MICs) as low as 1.18 µM. Two novel aminoalkylphenols were identified as particularly promising, after MIC and minimum bactericidal concentrations (MBC) determination against a panel of reference strain Gram-positive bacteria, and further confirmed against 40 clinical isolates (<i>Staphylococcus aureus</i>, <i>S. epidermidis</i>, <i>Enterococcus</i> <i>faecalis</i>,<i> E. faecium</i>, and<i> Listeria monocytogenes</i>). The same two aminoalkylphenols displayed low toxicity against two <i>in vivo</i> models (<i>Artemia salina</i> brine shrimp and <i>Saccharomyces cerevisiae</i>)<i>. </i>The <i>in vitro</i> cytotoxicity evaluation (on human keratinocytes and human embryonic lung fibroblast cell lines) of the same compounds was also carried out. They demonstrated a particularly toxic effect on the fibroblast cell lines, with IC<sub>50</sub> in the 1.7-5.1 mM range, thus narrowing their clinical use. The desired increase in the antibacterial properties of the alkylaminophenols, particularly indoline-derived phenolic Mannich bases, was reached by introducing an additional nitro group in the indolinyl substituent or by the replacement of a methyl by a bioisosteric trifluoromethyl substituent. Notably, the introduction of an additional nitro moiety did not confer added toxicity to the alkylaminophenols.</p>

49 EFFECTS OF CULTURE CONDITIONS AND GENE TRANSFECTION ON THE DEVELOPMENT OF BOVINE SOMATIC CELL NUCLEAR TRANSFER EMBRYOS

2013 ◽  
Vol 25 (1) ◽  
pp. 172
Author(s):  
P. Pärn ◽  
M. Plaas ◽  
M. Nõmm ◽  
Ü. Jaakma ◽  
S. Kõks
Keyword(s):  
Cell Lines ◽  
Gene Transfection ◽  
Fibroblast Cell ◽  
Culture Conditions ◽  
Fetal Fibroblast ◽  
Reconstructed Embryos ◽  
A Cell ◽  
Fetal Fibroblast Cell ◽  
Fibroblast Cell Lines

Somatic cell nucleus transfer (SCNT) and in vitro culture of reconstructed embryos are the pivotal steps for successful cloning and generation of transgenic cattle. The aim of the study was to determine the influence of different cell fusion parameters, maturation, and culture conditions and the type of a cell line (bovine fetal fibroblast cell lines with or without gene transfection) on SCNT blastocyst development. Slaughterhouse-derived oocytes were matured for 17 h in TCM-199 (Sigma, St. Louis, MO, USA) supplemented with 0.05 µg mL–1 of epidermal growth factor (EGF) and 15 IU mL–1 of hCG/eCG (Intervet, PG600) or 10 µg mL–1 of FSH and 12.5 mU mL–1 of LH (Sioux Biochemical Inc., Sioux Center, IA, USA). Four fetal fibroblast cell lines (4 to 5 passages) and identical cell lines transfected with plasmid containing either human erythropoietin, FSH, growth hormone, or insulin-coding cDNA under β-casein promoter (7 to 9 passages) were used for SCNT. Cell fusion was induced by 2 direct-current pulses in 0.5 or 0.2 micro fusion chambers (Eppendorf Multiporator) using one of the following treatments: 100V for 15 µs (F1), 65V for 25 µs (F2), 65V for 20 µs (F3; all in a 0.5-mm chamber), or 36V for 25 µs (F4; 0.2-mm chamber). Fused complexes were activated with 4 µg mL–1 of Ca-ionophore for 4 min and then incubated for 5 h in 2 mM DMAP. The embryos were cultured in SOFaaci medium (Holm et al. 1999) or in commercial SOF medium (Minitüb GmbH, Tiefenbach, Germany) for 7 days. Data were analysed by ANOVA and the chi-square test. The results of the study showed that the cleavage rate of the reconstructed embryos was influenced by the fusion regimen (P < 0.05) but not by the donor cell type (P < 0.05). Treatments F2 and F3 resulted in cleavage rates higher (P < 0.05) than F1 and F4 (77.2, 82.0, 62.8, and 63.1%, respectively). Blastocyst yield was not significantly influenced by the different in vitro maturation (IVM) media – altogether, addition of FSH/LH resulted in 14.6% (158/1079) and EGF + hCG/eCG in 13.2% (73/554) of blastocysts (P < 0.05). The combination of TCM-199 + FSH/LH and SOFaaci resulted in 19.6% (79/403) blastocysts compared with 12.4% (74/596) when the same IVM medium and commercial SOF were used (P < 0.05). The use of transgenic cell lines for cloning led to a lower overall blastocyst rate (10.9%, 38/348) than use of non-transfected cell lines (17.7%, 115/651; P < 0.05), whereas the differences were 5.6 and 4.1 percentage points for SOF and SOFaaci, respectively. There were no significant differences between the individual cell lines within a cell line type. In conclusion, the optimization of the fusion parameters and in vitro culture (IVC) conditions led to improved blastocyst yields. In vivo development potential of the generated embryos still has to be evaluated in further studies. This study was supported by Project EU29023 of Enterprise Estonia, CCRMB, targeted grant SF1080045s07, and grant P8001 from the Estonian University of Life Sciences.

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