scholarly journals Effect of phenobarbitone on the nucleocytoplasmic transport of ribonucleic acid in vitro

1976 ◽  
Vol 156 (3) ◽  
pp. 665-670 ◽  
Author(s):  
N Hazan ◽  
R McCauley
Keyword(s):  
Nucleic Acids ◽  
Ribonucleic Acid ◽  
Nucleocytoplasmic Transport ◽  
Assay System ◽  
Cellular Processes ◽  
Potential Site ◽  
Isolated Rat ◽  
Stimulation Of

The transport of nucleic acids from the nucleus to the cytoplasm is a potential site for modification of normal cellular processes by drugs and hormones. In this study the effect of phenobarbitone on nucleocytoplasmic transport of ribosomes was measured in an assay system in vitro. The transport of radioactive ribosomes from isolated rat hepatic nuclei to unlabelled post-microsomal supernatant was measured in rats treated with 80 mg of phenobarbitone/kg body wt. or saline 3h before death. With either treatment, transport was linear with time, and dependent on temperature and the presence of ATP. However, phenobarbitone treatment increased transport of ribonucleoproteins over saline-treated animals nearly twofold. The effect of phenobarbitone was mediated through the cytosol, but was not the result of altered stability of the RNA transported to the cytosol. Cycloheximide (5 mg/kg body wt.) given 3.5 h before death inhibited the stimulation of transport by phenobarbitone. The data indicate that phenobarbitone increased the transport of RNA by stimulating the synthesis of cytosol factors that regulate transport of RNA from the nucleus.

Insulin and incorporation of radioactivity into nucleic acid fraction of isolated diaphragm

1960 ◽  
Vol 199 (4) ◽  
pp. 719-721 ◽  
Author(s):  
Ira G. Wool
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Glucose Concentration ◽  
Orotic Acid ◽  
Acid Fraction ◽  
Rat Diaphragm ◽  
Isolated Rat

Insulin in vitro stimulated the incorporation into the nucleic acid fraction of isolated rat diaphragm of radioactivity from d-glucose-U-C14, adenine-8-C14 and orotic acid-6-C14; insulin had no effect on the incorporation of thymine-2-C14 into muscle nucleic acids. Insulin enhanced the incorporation into nucleic acids of C14 from adenine and orotic acid in the absence of added glucose, and incorporation of adenine-8-C14 was not influenced by glucose concentration over the range 0–600 mg %.

Localization of central sites of action of angiotensin II on ADH release in vitro

1978 ◽  
Vol 234 (2) ◽  
pp. F135-F140
Author(s):  
C. M. Gregg ◽  
R. L. Malvin
Keyword(s):  
Angiotensin Ii ◽  
Direct Action ◽  
Central Effect ◽  
The Central Nervous System ◽  
Sites Of Action ◽  
Isolated Rat ◽  
Supraoptic Nuclei ◽  
Stimulation Of

It is now thought that angiotensin II can stimulate antidiuretic hormone (ADH) release in vivo by a direct action in the central nervous system but it is not known whether the locus of stimulation is the hypothalamus or the neurohypophysis or both. Isolated rat neural lobes incubated for 10 min in buffer containing angiotensin II (200 ng/ml or 2 microgram/ml) did not increase ADH release compared to control values, but addition of KCl (60 mM) to the bath markedly stimulated ADH release. However, intact hypothalamoneurohypophysial systems (containing the supraoptic nuclei) incubated with angiotensin II (200 ng/ml or 2 microgram/ml) did show a pronounced stimulation of ADH release. The data support the hypothesis that angiotensin II, at least in vitro, has a central effect on ADH release which is at the level of the hypothalamus.

scholarly journals Effects of α-amanitin on the stimulation of prostatic ribonucleic acid polymerase by prostatic steroid–protein receptor complexes

1974 ◽  
Vol 140 (3) ◽  
pp. 565-567 ◽  
Author(s):  
P. Davies ◽  
K. Griffiths
Keyword(s):  
Ionic Strength ◽  
Rna Polymerase ◽  
Ribonucleic Acid ◽  
Selective Inhibitor ◽  
High Ionic Strength ◽  
Receptor Complexes ◽  
Protein Receptor ◽  
Stimulation Of

Stimulation of prostatic RNA polymerase in vitro by prostatic 17β-hydroxy-5α-androstan-3-one (5α-dihydrotestosterone)–receptor complexes has been previously reported. By use of the selective inhibitor, α-amanitin, we have shown that both nucleolar and extranucleolar RNA polymerase activities may be stimulated, but stimulation is abolished at high ionic strength.

THE EFFECT OF GROWTH HORMONE AND OF CORTISOL ON THE RESPONSE OF ISOLATED RAT DIAPHRAGM TO THE STIMULATING EFFECT OF INSULIN ON GLUCOSE UPTAKE AND ON INCORPORATION OF AMINO ACIDS INTO PROTEIN

1959 ◽  
Vol 18 (4) ◽  
pp. 395-408 ◽  
Author(s):  
K. L. MANCHESTER ◽  
P. J. RANDLE ◽  
F. G. YOUNG
Keyword(s):  
Amino Acids ◽  
Growth Hormone ◽  
Protein Synthesis ◽  
Glucose Uptake ◽  
Carbohydrate Metabolism ◽  
Rat Diaphragm ◽  
Pituitary Growth Hormone ◽  
Isolated Rat ◽  
Stimulation Of

SUMMARY 1. The effect of hypophysectomy, or of adrenalectomy, and injection of pituitary growth hormone (GH) or of cortisol, on the uptake of glucose and the incorporation of glycine into protein by isolated rat diaphragm, and the effect of the addition of insulin in vitro on these processes, has been studied. 2. Both hypophysectomy and adrenalectomy raised the uptake of glucose by isolated diaphragm, while treatment of the intact or of the hypophysectomized rat with GH, or of the intact or of the adrenalectomized rat with cortisol, depressed it. Although hypophysectomy and adrenalectomy did not influence the additional glucose uptake induced by 200 mu./ml. of insulin in vitro, both these operations enhanced the effect of 0·1–1·0 mu./ml. of insulin on glucose uptake by diaphragm in vitro. Treatment of the rat with GH or cortisol diminished the rise in glucose uptake of diaphragm induced by 0·1–1·0 mu./ml. insulin. 3. Hypophysectomy depressed, and administration of GH to the intact or hypophysectomized rat raised, the incorporation of glycine into protein of the isolated diaphragm, but neither of these operations altered the magnitude of the stimulation of incorporation induced by 1·0 mu./ml. insulin. 4. Adrenalectomy raised, and administration of cortisol to the intact or adrenalectomized rat depressed, the incorporation of glycine into protein of the isolated diaphragm; adrenalectomy enhanced, the injection of cortisol diminished, the effect of 1·0 mu./ml. insulin on these processes. 5. The possibility that GH directs insulin towards the stimulation of protein synthesis, in part by restraining the action of insulin on carbohydrate metabolism, is discussed.

scholarly journals 2P183 Asymmetric nucleocytoplasmic transport using a novel in vitro assay system

2004 ◽  
Vol 44 (supplement) ◽  
pp. S155
Author(s):  
A. okonogi ◽  
M. Hiroshima ◽  
S. Shiina ◽  
S. Kose ◽  
N. Imamoto ◽  
...  
Keyword(s):  
Nucleocytoplasmic Transport ◽  
In Vitro Assay ◽  
Assay System ◽  
Vitro Assay

scholarly journals Inhibition by α-amanitin of ribonucleic acid polymerase solubilized from rat liver nuclei

1970 ◽  
Vol 116 (2) ◽  
pp. 177-180 ◽  
Author(s):  
F. Novello ◽  
L. Fiume ◽  
F. Stirpe
Keyword(s):  
Rna Polymerase ◽  
Ammonium Sulphate ◽  
Rat Liver ◽  
Ribonucleic Acid ◽  
Isolated Rat Liver ◽  
Rat Liver Nuclei ◽  
Liver Nuclei ◽  
Isolated Rat

1. α-Amanitin inhibits in vitro the RNA polymerase solubilized from isolated rat liver nuclei. 2. In contrast with previous observations with whole nuclei, the inhibition occurs approximately to the same extent in the presence and in the absence of ammonium sulphate. 3. Evidence is presented that the toxin acts by interacting with the enzyme itself and not with DNA or other components.

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