scholarly journals The extraction and purification of a cysteine transfer ribonucleic acid from baker's yeast

1976 ◽  
Vol 153 (2) ◽  
pp. 429-435 ◽  
Author(s):  
N J Holness ◽  
G Atfield
Keyword(s):  
Particle Size ◽  
Ribonucleic Acid ◽  
Distribution Curve ◽  
Narrow Range ◽  
Baker’S Yeast ◽  
The Other ◽  
Baker's Yeast ◽  
Yeast Trna ◽  
Extraction And Purification ◽  
Support Medium

1. A modification of the RPC 1 system of A.D. Kelmers, G.D. Novelli & M.P. Stulberg (1965) (J. Biol. Chem. 240, 3979-3983) is described in which the support medium is a Celite of narrow range particle size treated with dichlorodimethylsilane. 2. By using this system an apparently pure preparation of tRNA Cys was isolated from baker's yeast tRNA. 3. This preparation accepted at least 60% of the theoretical quantity of [3-14C]cysteine in a conventional assay and failed to accept isoleucine, phenylalanine, proline, serine or tyrosine. 4. A theoretical countercurrent-distribution curve calculated by assuming a distribution coefficient K of 2.03 was in excellent agreement with the profiles of E260 and cysteine-acceptor ability after 537 transfers in the 1.85 M-phosphate/formamide/propan-2-ol system of C.M. Connelly & B.P. Doctor (1965) (J. Biol. Chem. 241, 715-719). 5. Chromatography of tRNA Cys on Bio-Gel P100 polyacrylamide beads afforded two components one of which was far less efficient than the other in accepting cysteine. The base compositions of the two were similar.

scholarly journals The nucleotide sequence of cysteine transfer ribonucleic acid from baker's yeast. Products of complete digestion with pancreatic ribonuclease and ribonuclease T1

1976 ◽  
Vol 153 (2) ◽  
pp. 437-446 ◽  
Author(s):  
N J Holness ◽  
G Atfield
Keyword(s):  
Nucleotide Sequence ◽  
Ribonucleic Acid ◽  
Baker’S Yeast ◽  
Baker's Yeast ◽  
Brief Treatment ◽  
Ribonuclease T1 ◽  
Pancreatic Ribonuclease ◽  
Adenylic Acid ◽  
Complete Digestion ◽  
Conventional Methods

1. The nucleotide chain of tRNA Cys from baker's yeast was readily split at the anticolon into two large fragments by brief treatment with ribonuclease T1.2. The whole molecule and the two derived large fragments were completely digested with (a) pancreatic ribonuclease and (b) ribonuclease T1. The fragments present in each of the digests were separated and sequenced by conventional methods. 3. The groups of fragments derived from the two methods of digestion were entirely compatible with each other. 4. The molecule is 75 nucleotides long, but, as isolated, lacks the terminal adenosine and the neighboring cytidylic acid residue. The minor nucleotides 1-methyladenylic acid, 7-methylguanylic acid, 5-methylcytidylic acid and N6 (γγ-dimethylallyl)adenylic acid (isopentenyladenylic acid) were identified.

Isolation of Seryl and Phenylalanyl Ribonucleic Acid Synthetases from Baker's Yeast*

Biochemistry ◽  
1965 ◽  
Vol 4 (7) ◽  
pp. 1434-1442 ◽  
Author(s):  
Maynard H. Makman ◽  
Giulio L. Cantoni
Keyword(s):  
Ribonucleic Acid ◽  
Baker’S Yeast ◽  
Baker's Yeast

scholarly journals The nucleotide sequence of cysteine transfer ribonucleic acid from baker's yeast. Identification of the products from partial degradation of the molecule and derivation of the complete sequence

1976 ◽  
Vol 153 (2) ◽  
pp. 447-454 ◽  
Author(s):  
N J Holness ◽  
G Atfield
Keyword(s):  
Nucleotide Sequence ◽  
Ribonucleic Acid ◽  
Complete Sequence ◽  
Baker’S Yeast ◽  
Baker's Yeast ◽  
Brief Treatment ◽  
Yeast Identification ◽  
Pancreatic Ribonuclease ◽  
Correct Sequence ◽  
Partial Degradation

1. A series of large oligonucleotide fragments derived from tRNA Cys, were separated chromatographically and the sequence of each was deduced by examination of the products of digestion with pancreatic and T1 ribonucleases. 2. The location of the specific cleavage points in the nucleotide chain was similar to that produced by brief treatment with pancreatic ribonuclease. 3. The fragments could be arranged into two alternative sequences. The correct sequence was deduced by the sequential removal and identification of the first nine nucleotides from the 3′-end of the terminal half of the molecules.

High-Yield Extraction and Purification of Glutathione Reductase from Baker's Yeast

1991 ◽  
Vol 21 (2-3) ◽  
pp. 175-185 ◽  
Author(s):  
Ying-Chieh Tsai ◽  
Tzu-Yu Yang ◽  
Shiow-Wen Cheng ◽  
Shui-Nin Li ◽  
Yng-Jiin Wang
Keyword(s):  
Glutathione Reductase ◽  
Baker’S Yeast ◽  
High Yield ◽  
Baker's Yeast ◽  
Extraction And Purification
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