scholarly journals A new method for the measurement of protein turnover

1975 ◽  
Vol 148 (1) ◽  
pp. 119-127 ◽  
Author(s):  
T J Humphrey ◽  
D D Davies
Keyword(s):  
Amino Acids ◽  
Carbon Atom ◽  
Protein Degradation ◽  
Culture Medium ◽  
Lemna Minor ◽  
Total Soluble Protein ◽  
Short Exposure ◽  
Protein Amino Acids ◽  
A New Technique

A new technique for the determination of rate constants of protein degradation is described. By using the method, half-lives of total soluble protein of Lemna minor during growth on full culture medium and distilled water were measured. The method involves incubating Lemna on a growth medium containing 3H2O. After a short exposure (20 min) to 3H-labelled culture medium, 3H was found in soluble amino acids, especially aspartate, glutamate, glutamine and alanine. After transfer to a 3H-free medium for 30 min, 80% of the 3H originally present in soluble amino acids was lost. These results suggest that 3H enters and leaves amino acids at the α-carbon atom, a conclusion supported by the observed labelling of glutamates. The exchange of H and 3H on the α-carbon atom is catalysed by transaminases and the speed of this exchange ensures that when the 3H2O is removed, the 3H in free amino acids is rapidly lost, thereby eliminating problems connected with metabolic pools and recycling. After an exposure of 20 min to 3H-labelled medium all protein amino acids, except for arginine, were found to be radioactive. The loss of radioactivity from protein amino acids was used to measure protein degradation.

The Oocytes of the Goby Pomatoschistus minutus. III. Determination of Amino Acid Component

1980 ◽  
Vol 35 (11-12) ◽  
pp. 1094-1095
Author(s):  
Rüdiger Riehl
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Glutamic Acid ◽  
Acid Content ◽  
Amino Acid Content ◽  
Acid Component ◽  
Pomatoschistus Minutus ◽  
Protein Amino Acids ◽  
Amino Acid Component

Abstract The oocytes of the marine goby Pomatoschistus minutus were analyzed for their amino acid content. Most of the amino acids exist as protein, only a little part is free or peptide-bound. Among the protein-bound amino acids, high levels of glutamic acid, proline, alanine, aspartic acid, valine and leucine were detected. These represent more than 60% of the protein amino acids. Among the free acids, glutamic acid, serine and alanine, are dominant. There are no certain proofs of the occurrence of peptide pools in the oocytes of Pomatoschistus minutus.

scholarly journals Arthroderma tuberculatum and Arthroderma multifidum Isolated from Soils in Rook (Corvus frugilegus) Colonies as Producers of Keratinolytic Enzymes and Mineral Forms of N and S

2020 ◽  
Vol 17 (24) ◽  
pp. 9162
Author(s):  
Justyna Bohacz ◽  
Michał Możejko ◽  
Ignacy Kitowski
Keyword(s):  
Amino Acids ◽  
Culture Medium ◽  
Agricultural Practice ◽  
Ammonium Ions ◽  
Mineral N ◽  
Corvus Frugilegus ◽  
Keratinolytic Fungi ◽  
Sulfate Release ◽  
Proteins And Peptides

Keratinolytic fungi representing the genus Arthroderma that were isolated from the soils of a rook (Corvus frugilegus) colony were used as biological agents for the disposal of waste feathers. The aim of this study was to assess the abilities of Arthroderma tuberculatum and Arthroderma multifidum fungi with a varied inflow of keratin matter to biodegrade waste feathers. The evaluation was based on the determination of feather mass loss, the activity of keratinolytic enzymes, and the content of mineral N and S forms. It was found that the activity of protease released by the fungi contributed to an increase in the level of soluble proteins and peptides and the concentration of ammonium ions, as well as alkalization of the culture medium. Keratinase activity was significantly correlated with sulfate release, especially in A. tuberculatum cultures. The strains of A. tuberculatum fungi isolated from the soil with the highest supply of organic matter, i.e., strains III, IV, and V, had the lowest enzymatic activity, compared to the A. multifidum strains, but they released mineral nitrogen and sulfur forms that are highly important for fertilization, as well as nutritionally important peptides and amino acids. A. tuberculatum strains can be used for the management of waste feathers that can be applied in agricultural practice.

scholarly journals Determination of the effectiveness of microbiological preparations and bioavailable nutrients while growing a vegetative apple rootstock

2021 ◽  
Vol 34 ◽  
pp. 05007
Author(s):  
Tatiana Prichko ◽  
Vladimir Mager ◽  
Roman Oplachko
Keyword(s):  
Amino Acids ◽  
Survival Rate ◽  
Soil Microorganisms ◽  
Natural Soil ◽  
Apple Rootstock ◽  
Biometric Parameters ◽  
Protein Amino Acids ◽  
Effect Of Treatment ◽  
The Impact

This article presents the results of a study of the effect of treatment of the root system of the M9 stock with the microbiological preparation Agrinos 1, which is a consortium of natural soil microorganisms - more than 80 strains from 10 families in combination with the preparation Agrinos 2, containing a complex of bioavailable nutrients (macro-, microelements, protein, amino acids, chitin, chitosan) in order to activate metabolic processes, reduce the impact of stress on the plant. The effect of biological products on the survival rate of the stock in the nursery, an increase in the yield and standardization of offshoots, and an improvement in their biometric parameters was established.

scholarly journals The measurement of protein turnover by density labelling

1975 ◽  
Vol 152 (2) ◽  
pp. 409-416 ◽  
Author(s):  
Alain Boudet ◽  
Thomas J. Humphrey ◽  
David D. Davies
Keyword(s):  
Amino Acids ◽  
Protein Degradation ◽  
Plant Tissue ◽  
Protein Turnover ◽  
Half Life ◽  
Lemna Minor ◽  
Enzyme Activation ◽  
Considerable Improvement ◽  
Minor Protein ◽  
Isopycnic Centrifugation

A method for measuring the rate of protein degradation in plant tissue is described. The method uses density labelling to avoid difficulties associated with compartmentation and recycling of amino acids. Although the technique cannot be readily adapted to measure the rate of degradation of single proteins, it avoids difficulties of interpretation due to enzyme activation or inactivation. Values for the half-life of Lemna minor protein obtained by this method are compared with values obtained by a number of other methods. To obtain satisfactory results it was necessary to improve the method of isopycnic centrifugation in CsCl gradients. A considerable improvement was achieved by using KBr gradients, and the advantages of using KBr rather than CsCl for the separation of density-labelled protein are discussed.

Determination of the D and L isomers of some protein amino acids present in soils

1977 ◽  
Vol 49 (1) ◽  
pp. 2-7 ◽  
Author(s):  
Glenn E. Pollock ◽  
C. N. Cheng ◽  
S. E. Cronin
Keyword(s):  
Amino Acids ◽  
Protein Amino Acids

Determination of the rate of acidic catalyzed racemization of protein amino acids

1981 ◽  
Vol 1981 (3) ◽  
pp. 354-365 ◽  
Author(s):  
Hartmut Frank ◽  
Wolfgang Woiwode ◽  
Graeme Nicholson ◽  
Ernst Bayer
Keyword(s):  
Amino Acids ◽  
Protein Amino Acids
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