Protein synthesis in the cotyledons of Pisum sativum L. Protein factors involved in the binding of phenylalanyl-transfer ribonucleic acid to ribosomes

1975 ◽  
Vol 145 (3) ◽  
pp. 417.b1-417.b1
Author(s):  
G. N. Wells ◽  
L. Beevers
Keyword(s):  
Protein Synthesis ◽  
Pisum Sativum ◽  
Ribonucleic Acid ◽  
L Protein ◽  
Pisum Sativum L

scholarly journals Protein synthesis in the cotyledons of Pisum sativum L. Protein factors involved in the binding of phenylalanyl-transfer ribonucleic acid to ribosomes*

1974 ◽  
Vol 139 (1) ◽  
pp. 61-69 ◽  
Author(s):  
Gary N. Wells ◽  
Leonard Beevers
Keyword(s):  
Protein Synthesis ◽  
Complex Formation ◽  
Pisum Sativum ◽  
Ribonucleic Acid ◽  
Ternary Complex ◽  
Supernatant Fraction ◽  
Soluble Factors ◽  
Pisum Sativum L ◽  
Sensitive Site ◽  
Millipore Filters

1. Proteinaceous factors contained in a 0.5m-KCl extract of ribosomes from pea cotyledons form a ternary complex at 0°C with [14C]phenylalanyl-tRNA and poly(U). The complex is measured by its quantitative retention on Millipore filters. 2. Complex-assembly is optimal at 5mm-Mg2+ and is independent of GTP and ribosomes. 3. The addition of ribosomes is required to stabilize the complex at 34°C. The complex binds to a puromycin-sensitive site on the ribosome. 4. Soluble factors from the 250000g supernatant of pea cotyledon form a Millipore-retainable complex dependent on GTP and ribosomes. 5. Complex-formation by soluble factors has a Mg2+ optimum of 10–12mm and forms a puromycin-insensitive complex with ribosomes. 6. The function of the ribosomal protein factors and the supernatant fraction in initiation of protein synthesis is discussed.

Long-lived Messenger RNA and its Relationship to Protein Synthesis During Germination of Pea (Pisum sativum L.) Seeds

1986 ◽  
Vol 57 (6) ◽  
pp. 771-781 ◽  
Author(s):  
S. C. Greenway ◽  
G. M. Strangeway ◽  
D. Grierson ◽  
J. A. Bryant
Keyword(s):  
Protein Synthesis ◽  
Pisum Sativum ◽  
Messenger Rna ◽  
Pisum Sativum L

scholarly journals EFFECTS OF 60COBALT IONIZING IRRADIATION ON GROWTH AND POLYSOME CONTENT IN PISUM SATIVUM L. `ALASKA'

HortScience ◽  
1992 ◽  
Vol 27 (12) ◽  
pp. 1261d-1261
Author(s):  
Jody A. Goodrich
Keyword(s):  
Protein Synthesis ◽  
Pisum Sativum ◽  
Plant Cells ◽  
Root Tip ◽  
Nuclear Accidents ◽  
Ionizing Irradiation ◽  
Pisum Sativum L ◽  
Living Organisms ◽  
Injurious Effects

This research tested the hypothesis that 60Co ionizing irradiation degrades polysomes to monosomes, a process that reduces growth of Pisum sativum seedlings. Dry and imbibed seeds and 5-day-old seedlings were exposed to 1.8, 3.6, 7.2, 14.4, or 28.8 krad of 60Co irradiation. Immediately after irradiation treatments, dry and imbibed seeds were planted, and later seedlings were harvested and analyzed. Five 1-cm root-tip samples from 5-day-old seedlings were crushed and layered onto 15% to 60% sucrose gradients and centrifuged for 55 min. The samples were processed through an ISCO ultraviolet chart maker. The monosome and polysome weights were read and analyzed. The monosome content was greater in the irradiated 5-day-old seedlings than in seedlings from dry and imbibed seeds. The growth of 5-day-old irradiated seedlings and seedlings from imbibed seeds was less than that of seedlings from dry seeds. The reduced growth of the irradiated seedlings suggests damage to the polysomes. When protein synthesis in plant cells is altered, perhaps through RNA decoding mechanisms, growth may be partially or completely arrested. Using sensitive plants to establish the injurious effects of ionizing irradiation on living organisms can educate and alert society to the detrimental effects of overexposure to irradiation such as that caused by nuclear accidents.

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