scholarly journals A solid-phase radioimmunoassay for plasma progesterone

1974 ◽  
Vol 143 (1) ◽  
pp. 219-231 ◽  
Author(s):  
Kailas K. Dighe ◽  
William M. Hunter
Keyword(s):  
Menstrual Cycle ◽  
Solid Phase ◽  
Plasma Progesterone ◽  
Fourfold Increase ◽  
Double Antibody ◽  
Antibody Method ◽  
Normal Menstrual Cycle ◽  
Covalently Linked ◽  
Excretion Rates ◽  
Routine Assay

A detailed procedure is presented for the assay of plasma progesterone. The routine assay is based on the use of antiserum which is covalently linked to microcrystalline cellulose, the double-antibody method being used as a reference separation system. This procedure gives high precision accompanied by small and acceptable losses of antiserum titre but without loss of sensitivity when compared with the double-antibody method. Ethanol is first added to the plasma (10vol. of plasma+1vol. of ethanol) after which a single extraction with light petroleum yields a constant recovery [92.4±1.2 (s.d.)% of added [3H]progesterone] and obviates the need for tracer recoveries on each sample being assayed. Distortions of the response curve owing to solvent residues have been almost eliminated. The assay can measure progesterone at all stages of the menstrual cycle when volumes of 200μl of plasma are used and this permits the detection of the periovulatory rise at its inception. Detailed specificity studies are presented for the assay end point itself and these are related to the responses to be expected in extracts of plasma. Progesterone-like activity was found in urine and a fourfold increase in excretion rates was observed between the follicular and luteal phase of the normal menstrual cycle.

Changes in plasma LRH during the normal menstrual cycle in women

1980 ◽  
Vol 93 (3) ◽  
pp. 257-263 ◽  
Author(s):  
A. Miyake ◽  
Y. Kawamura ◽  
T. Aono ◽  
K. Kurachi
Keyword(s):  
Menstrual Cycle ◽  
Luteal Phase ◽  
Plasma Concentrations ◽  
Mean Value ◽  
Lh Surge ◽  
Double Antibody ◽  
Normal Menstrual Cycle ◽  
The Mean ◽  
Plasma Oestradiol

Abstract. The plasma concentrations of immunoreactive LRH, LH, FSH, oestradiol and progesterone were measured daily by a sensitive double antibody radioimmunoassay during 12 cycles of 8 normal cyclic women. The mean (± se) immunoreactive LRH levels in the follicular and luteal phase except the immunoreactive LRH peaks during normal cycles were 4.18 ± 0.38 pg/ml and 4.50 ± 0.45 pg/ml, respectively. The immunoreactive LRH peaks were observed in 11 of 12 cycles, appearing on day −4 to −1 from the LH surge in 9 cycles and on day +1 and +2 in 2 cycles. The mean value of immunoreactice LRH peaks was 42.0 ± 11.4 pg/ml with range of 12 to 154 pg/ml. The immunoreactive LRH peak lasted for one day in 10 cycles and for 4 days in one cycle. The immunoreactive LRH peaks in different cycles of the same women did not occur on the same day relative to the LH peak. The plasma immunoreactive LRH levels measured every 10 min for 40 min periods every day in normal cyclic women during the ovulatory phase showed slight, but not significant fluctuations. Plasma oestradiol levels began to increase on day −6, reaching a peak on day − 1, and were followed by peaks of LH and FSH. These data indicate that increase in serum oestradiol was followed by release of LRH from the hypothalamus and pre-ovulatory discharge of gonadotrophins from the pituitary.

PLASMA RENIN ACTIVITY, PLASMA RENIN SUBSTRATE AND URINARY ALDOSTERONE EXCRETION IN THE MENSTRUAL CYCLE IN RELATION TO THE CONCENTRATION OF PROGESTERONE AND OESTROGENS IN THE PLASMA

1972 ◽  
Vol 71 (3) ◽  
pp. 519-529 ◽  
Author(s):  
Johan A. Sundsfjord ◽  
A. Aakvaag
Keyword(s):  
Menstrual Cycle ◽  
Plasma Renin Activity ◽  
Luteal Phase ◽  
Plasma Renin ◽  
Renin Activity ◽  
Renin Substrate ◽  
Plasma Progesterone ◽  
Failure Group ◽  
Normal Menstrual Cycle ◽  
Normal Increase

ABSTRACT The relative importance of endogenous progesterone and oestrogens on the rise in plasma renin activity and urinary aldosterone excretion in the luteal phase of the menstrual cycle has been investigated in two groups of young women. One group (luteal group) had plasma progesterone levels of 7–19.5 ng/ml (mean 12.3; sem 1.3) in the second half of the cycle. The other group (luteal failure group) had plasma progesterone levels of 1.5–3.0 ng/ml, (mean 2.1; sem 0.16), on the corresponding days in the second half of the cycle. The plasma oestrogen levels and the oestriol excretion did not differ between the groups at any stage of the cycle. In the luteal group the normal increase in plasma progesterone and plasma oestrogens and in oestriol excretion was accompanied by a doubling in plasma renin activity and urinary aldosterone excretion, whereas the plasma renin substrate levels did not change significantly. In the luteal failure group, no significant increase was found in either plasma renin activity or in urinary aldosterone excretion, in spite of the unchanged plasma oestrogen levels and oestriol excretion. In this group too the plasma renin substrate levels showed no change from the first to the second half of the cycle. It is concluded that the rise in plasma renin activity, plasma angiotensin II concentration and urinary aldosterone excretion in the luteal phase of the normal menstrual cycle is caused by increased progesterone production.

STUDIES ON THE PATTERN OF CIRCULATING STEROIDS IN THE NORMAL MENSTRUAL CYCLE

1976 ◽  
Vol 81 (1) ◽  
pp. 133-149 ◽  
Author(s):  
R. Guerrero ◽  
T. Aso ◽  
P. F. Brenner ◽  
Z. Cekan ◽  
B.-M. Landgren ◽  
...  
Keyword(s):  
Menstrual Cycle ◽  
Luteinizing Hormone ◽  
Plasma Levels ◽  
The Other ◽  
Plasma Progesterone ◽  
Proliferative Phase ◽  
Normal Menstrual Cycle ◽  
Menstruating Women ◽  
The Individual ◽  
The Relationship

ABSTRACT In an attempt to analyze the multiple changes and interactions in circulating steroid levels in the peri-ovulatory and peri-menstrual periods, the plasma levels of immunoreactive luteinizing hormone (LH), progesterone and unconjugated pregnenolone, dehydroepiandrosterone, testosterone, oestradiol and oestrone were assayed daily during a complete cycle in 17 normally menstruating women. In 14 of the 17 subjects studied androstenedione and unconjugated dihydrotestosterone were also estimated. The day of the LH-peak and the first day of menstruation, respectively, were used to synchronize the peri-ovulatory and peri-menstrual plasma levels of the various steroids. With the exception of dehydroepiandrosterone and dihydrotestosterone, the plasma levels of all steroids exhibited significant, but different changes during the cycle. Testosterone levels showed a slight but significant increase around the LH-peak, whereas the levels of pregnenolone and androstenedione were higher in the post-ovulatory than in the pre-ovulatory periods. The levels of oestradiol and oestrone, as well as the ratios of oestradiol to oestrone gradually increased from the low values observed in the early proliferative phase to pre-ovulatory peak values. The relationship between peaks of oestradiol and oestrone and that of LH exhibited great individual variation. The same was true for the individual oestradiol to oestrone ratios. The combination of several steroidal signals did not improve the predictive value of the analyses. However, an increase of individual progesterone values by at least 0.35 ng/ml from the day preceding the LH-peak to the day of the LH-peak was observed in 13 of the 17 subjects. It is suggested that for the early detection of the LH surge and prediction of the subsequent ovulation daily assays of plasma progesterone are of more value than the assay of the other steroids investigated.

scholarly journals A Sensitive, Specific, Solid-Phase Enzymeimmunoassay for Plasma Progesterone

1981 ◽  
Vol 18 (1) ◽  
pp. 42-47 ◽  
Author(s):  
Brian G Joyce ◽  
Ann H Othick ◽  
Graham F Read ◽  
Diana Riad-Fahmy
Keyword(s):  
Solid Phase ◽  
World Health ◽  
Plasma Samples ◽  
Plasma Progesterone ◽  
Department Of Health ◽  
The United Kingdom ◽  
Freeze Dried ◽  
Enzyme Label ◽  
Covalently Linked ◽  
Health Organization

A homologous enzymeimmunoassay (EIA) for plasma progesterone, using a horseradish peroxidase conjugate as enzyme label and an antiserum raised against a progesterone-11α-hemisuccinyl/BSA conjugate, is described. The antiserum was covalently linked to microcrystalline cellulose to facilitate separation of bound and free steroid; this solid-phase antiserum was stable for at least nine months when stored at 4°C. The freeze-dried enzyme label is also stable, having retained both enzymic and immunological activity for about four years. The EIA developed was specific and had the sensitivity (4·8 pg/tube) required for determining progesterone concentrations in plasma samples collected at any time during the menstrual cycle. EIA of plasma samples provided results which were in good agreement with a well validated radioimmunoassay (RIA). The specificity and inter- and intra-assay coefficients of variation in the EIA were strictly comparable with those of the RIA. The method described has been in use for two years and has been assessed in external quality assurance programmes established by the World Health Organization and the United Kingdom Department of Health and Social Security.

THE RELATION BETWEEN PLASMA PROGESTERONE AND TOTAL URINARY OESTROGENS FOLLOWING INDUCTION OF OVULATION IN WOMEN

1969 ◽  
Vol 62 (1) ◽  
pp. 89-97 ◽  
Author(s):  
Elof D. B. Johansson ◽  
Carl Gemzell
Keyword(s):  
Menstrual Cycle ◽  
Luteal Phase ◽  
Single Injection ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Human Pituitary ◽  
Plasma Progesterone ◽  
Before And After ◽  
Normal Menstrual Cycle ◽  
Different Levels

ABSTRACT Plasma progesterone levels have been measured before and after a single injection of 9000 IU of human chorionic gonadotrophin (HCG)* at different levels of total oestrogen excretion (TE) in 67 treatments with human pituitary gonadotrophin (HPG) in amenorrhoic women. With a TE excretion of less than 70 μg/24 h the rise in plasma progesterone was similar to that found in a normal menstrual cycle indicating the formation of only one corpus luteum. With more than 70 μg/24 h of TE several corpus lutea was formed as judged by the rise in plasma progesterone levels. No further increase was seen above 300 μg/24 h. The luteal phase was shorter than normal in the group with 75–300 μg/24 h of TE.

PROGESTERONE IN THE HUMAN PERIPHERAL BLOOD IN THE PREOVULATORY PERIOD OF THE MENSTRUAL CYCLE

1967 ◽  
Vol 55 (1) ◽  
pp. 91-96 ◽  
Author(s):  
Benno Runnebaum ◽  
Josef Zander
Keyword(s):  
Menstrual Cycle ◽  
Corpus Luteum ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Progesterone Concentration ◽  
Plasma Progesterone ◽  
Progesterone Secretion ◽  
Graafian Follicle ◽  
The Mean ◽  
Mean Concentration

ABSTRACT Progesterone was determined and identified in human peripheral blood during the preovulatory period of the menstrual cycle, by combined isotope derivative and recrystallization analysis. The mean concentration of progesterone in 1.095 ml of plasma obtained 9 days before ovulation was 0.084 μg/100 ml. However, the mean concentration of progesterone in 1.122 ml of plasma obtained 4 days before ovulation was 0.279 μg/100 ml. These data demonstrate a source of progesterone secretion other than the corpus luteum. The higher plasma-progesterone concentration 4 days before ovulation may indicate progesterone secretion of the ripening Graafian follicle of the ovary.

SOME OBSERVATIONS ON PREGNANEDIOL EXCRETION DURING THE NORMAL MENSTRUAL CYCLE

1957 ◽  
Vol 24 (3_Suppl) ◽  
pp. S207
Author(s):  
A. Klopper
Keyword(s):  
Menstrual Cycle ◽  
Corpus Luteum ◽  
New Method ◽  
Menstrual Bleeding ◽  
Normal Menstrual Cycle ◽  
The Relationship ◽  
New View

Abstract The changes in view on the significance and amount of urinary pregnanediol in the menstrual cycle are reviewed; in particular the effects of the discovery that the adrenals in both sexes normally contribute to the urinary pregnanediol. Pregnanediol excretion during the menstrual cycle was studied by means of a new method of assay (Klopper et al., 1955) and the results applied to present day concepts of the growth and duration of the corpus luteum. The relationship between pregnanediol excretion and ovulation or the onset of menstrual bleeding was studied. A new view is put forward on the influence of age and parity on the production of progesterone by the corpus luteum.

Hormone levels in the normal menstrual cycle

Reproduction ◽  
1971 ◽  
Vol 27 (3) ◽  
pp. 481-484 ◽  
Author(s):  
J Newton ◽  
D Joyce ◽  
B Pearce ◽  
C Revell ◽  
J Tyler
Keyword(s):  
Menstrual Cycle ◽  
Hormone Levels ◽  
Normal Menstrual Cycle
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